Genetic manipulation of non‐solvent‐producing microbial species for effective butanol production

Abstract

Butanol is widely used as an important bulk chemical and is a potential biofuel. The depletion of fossil fuels and advances in synthetic biotechnology have led to renewed interest in the biological production of butanol. Solventogenic Clostridium was commonly used to produce butanol through traditional acetone‐butanol‐ethanol (ABE) fermentation. However, its relatively slow growth rate, low butanol tolerance, and poor production efficiency have hindered the further application of this procedure. Recently, other promising industrial hosts, including Escherichia coli, Saccharomyces cerevisiae, and Clostridium tyrobutyricum, have been studied for potential use in the production of butanol. This review comprehensively summarizes the advantages and challenges of different non‐solvent strains for butanol production to identify better the ideal non‐solvent hosts for butanol production. Strategies to further increase butanol production are also proposed. © 2020 Society of Chemical Industry and John Wiley & Sons, Ltd.

Genomic analysis and plant growth-promoting potential of a Serratia marcescens isolated from food

“…Butanol is a sustainable technology for alternate and renewable energy, being less corrosive and with higher energy content per unit mass than traditional fuels. The butanol potential includes its use as a solvent and a platform chemical in the cosmetic and pharmaceutical industry (Lv et al, 2021).…”

Alone or together? A review on pure and mixed microbial cultures for butanol production

“…E. coli, P. putida) [48,49]. A more detailed review on non-solvent-producing hosts can be found elsewhere [50]. Another challenge Clostridium species face is their glucose requirements and low ability to degrade other sugars and carbohydrates.…”