Alone or together? A review on pure and mixed microbial cultures for butanol production

Pinto, Tiago; Flores-Alsina, Xavier; Gernaey, Krist V.; Junicke, Helena

doi:10.1016/j.rser.2021.111244

Cited by 33 publications

(14 citation statements)

References 116 publications

(123 reference statements)

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“…For example, Li et al (2021) described the potential of using membrane technologies. Additionally, Step 3 was assumed to be pure culture fermentations; however, the review by Pinto et al (2021) has documented that mixed cultures might bring some advantages. The superstructure does not include these alternatives due to the, so far, lack of maturity and data availability.…”

Section: Multi-objective Optimization Resultsmentioning

confidence: 99%

Optimal Conversion of Organic Wastes to Value-Added Products: Toward a Sustainable Integrated Biorefinery in Denmark

et al. 2022

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It is crucial to leave behind the traditional linear economy approach. Shifting the paradigm and adopting a circular (bio)economy seems to be the strategy to decouple economic growth from continuous resource extraction. To this end, producing bio-based products that aim to replace a part, if not all, of the fossil-based chemicals and fuels is a promising step. This can be achieved by using multi-product integrated biorefineries that convert organic wastes into chemicals, fuels, and bioenergy to optimize the use and close the materials and energy loops. To further address the development and implementation of organic waste integrated biorefineries, we proposed the open-source organic waste to value-added products (O2V) model and multi-objective optimization tool. O2V aims to provide a quick and straightforward holistic assessment, leading to identifying optimal or near-optimal design, planning, and operational decisions. This model not only prioritizes economic benefits but also takes on board the other pillars of sustainability. The proposed tool is built on a comprehensive superstructure of processing alternatives that include all stages concerning the conversion of organic waste to value-added products. Furthermore, it has been framed and formulated in a “plug-and-play” format, where, when required, the user only needs to add new process data to the structured information database. This database integrates data on (i) new processes (e.g., different conversion technologies), (ii) feedstocks (e.g., composition), and (iii) products (e.g., prices), among others. Due to Denmark’s high availability of organic waste, implementing a second-generation integrated biorefinery in Denmark has been chosen as a realistic showcase. The application of O2V efficiently led to the identification of trade-offs between the different sustainability angles. Thus, it made it possible to determine early-stage decisions regarding product portfolio, optimal production process, and related planning and operational decisions. Henceforth, it has been demonstrated that applying O2V aids in shifting the fossil to bio-based production, thereby contributing to the switch toward a circular bioeconomy.

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Section: Multi-objective Optimization Resultsmentioning

confidence: 99%

Optimal Conversion of Organic Wastes to Value-Added Products: Toward a Sustainable Integrated Biorefinery in Denmark

et al. 2022

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“…Both of the bacteria has different optimal temperature, but they successfully cultured both bacteria at 30 °C. Other than that, another research of co-culture between Bacillus subtilis and Clostridium butylicum resulted in a 6.5-fold in ABE production compared to single culture (Clostridium butylicum) [74].…”

Section: Mixed Microbe Culturementioning

confidence: 97%

“…However, the co-culture between a cellulolytic bacterium and solventogenic bacteria might develop a new problem to verify the optimal growth of the co-culture. Pinto et al [74] have done an investigation, using Clostridium thermocellum and Clostridium saccharoperbutylacetonicum as cellulolytic and a butanol-producing bacterium respectively. Both of the bacteria has different optimal temperature, but they successfully cultured both bacteria at 30 °C.…”

Section: Mixed Microbe Culturementioning

confidence: 99%

Lignocellulosic Biomass – A Sustainable Feedstock for Acetone-Butanol-Ethanol Fermentation

Mahalingam

Abdulla

Sani

et al. 2022

Period. Polytech. Chem. Eng.

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Biobutanol has been identified as a promising future biofuel. However, generally the extraction and separation of biobutanol from the fermentation mixture is a costly process. Therefore, the idea of using acetone-butanol-ethanol (ABE) mixture directly as biofuel were proposed to eliminate the recovery process. ABE has been identified as a promising future biofuel. The feedstocks play an important role in the feasibility of ABE as a fuel. Lignocellulosic biomass is seen as a promising feedstock for the production of biofuels. Thus, in this review, ABE biofuel is been summarized from three aspects namely (i) selection of feedstocks, (ii) microbial selection and (iii) hydrolysis, fermentation, and purification techniques. Anaerobic fermentation together with commonly employed recovery processes are discussed in the second part of this review. This review concludes with different challenges and future research in ABE fermentation that can pave the way for future commercialization of this promising biofuel.

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“…Compared to ethanol, butanol has a higher energy density and is less hygroscopic, less volatile, and less corrosive, making it more compatible with current infrastructures for gasoline storage and transportation [5]. Furthermore, butanol can be produced from a wide range of organic and industrial wastes; while much research is focused on solid residues, wastewater is also a prime candidate for butanol production [6,7].…”

Section: Introductionmentioning

confidence: 99%

Shaping an Open Microbiome for Butanol Production through Process Control

et al. 2022

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The growing awareness of limited resource availability has driven production systems towards greater efficiencies, and motivated the transition of wastewater treatment plants to water resource recovery facilities. Open microbiome fermentation offers a robust platform for resource recovery, due to its higher metabolic versatility, which is capable of dealing with even dilute residual liquid streams. Organic matter, e.g., fatty acids, lost in these streams can potentially be recovered into higher value chemicals such as alcohols. This study aims to shape an open microbiome towards butanol production from butyrate and hydrogen through pH control and continuous hydrogen supply. Two sets of experiments were conducted in Scott bottles (1 L) and a lab-fermenter (3 L). The open microbiome produced up to 4.4 mM butanol in 1 L bottles. More promising conversions were obtained when up-scaling to a lab-fermenter with pH control and an increased hydrogen partial pressure of 2 bar; results included a butanol concentration of 10.9 mM and an average volumetric productivity of 0.68 mmol L−1 d−1 after 16 days. This corresponds to 2.98- and 4.65-fold increases, respectively, over previously reported values. Thermodynamic calculations revealed that product formation from butyrate was unfeasible, but energetically favorable from bicarbonate present in the inoculum. For the first time, this study provides insights regarding the community structure of an open microbiome producing butanol from butyrate and hydrogen. DNA sequencing combined with 16S rRNA gene amplicon analysis showed high correlation between Mesotoga spp. and butanol formation. Microbial diversity can also explain the formation of by-products from non-butyrate carbon sources.

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Alone or together? A review on pure and mixed microbial cultures for butanol production

Cited by 33 publications

References 116 publications

Optimal Conversion of Organic Wastes to Value-Added Products: Toward a Sustainable Integrated Biorefinery in Denmark

Optimal Conversion of Organic Wastes to Value-Added Products: Toward a Sustainable Integrated Biorefinery in Denmark

Lignocellulosic Biomass – A Sustainable Feedstock for Acetone-Butanol-Ethanol Fermentation

Shaping an Open Microbiome for Butanol Production through Process Control

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