Genetic location of the Bacillus subtilis sup-3 suppressor mutation

Abstract

The sup-3 suppressor mutation of Bacillus subtilis has been located between the aroI and mtlB loci by PBS-1 phage transduction.

Structure and organization of genes for transfer ribonucleic acid in Bacillus subtilis.

“…References are as follows: Ile, Ala, rrnO, and rrnA (37); rrnB, rrnC, rrnG-I (3); six-tRNA-gene cluster (sequenced published by Wawrousek and Hansen, 57; mapped by K. Bott, personal communication); 21-tRNA-gene cluster (sequence published by Green and Vold [16] and Wawrousek et al [59]; mapped by Stewart et al. [52]); sup-3 (20,31); sup44 (31). two suppressor mutations have been mapped (20, 31), both at position 30, (Fig.…”

Defective specialized SP beta transducing bacteriophages of Bacillus subtilis that carry the sup-3 or sup-44 gene

“…In B. subtilis the mutations sup-3 and sup-44 apparently suppress nonsense mutations (5,8,11). No information is available on the nature of the codons suppressed or the biochemical mechanism of suppression in B. subtilis (11).…”