Defective specialized SP beta transducing bacteriophages of Bacillus subtilis that carry the sup-3 or sup-44 gene

Abstract

We isolated defective specialized transducing phages of SP,B that carry one of the extracistronic suppressors, sup-3 or sup-44. Lysates containing these phages can be used in a simple spot test to determine whether an auxotrophic mutation can be suppressed. The sup-3 and sup-44 mutations are distinct, in that their suppression patterns differ for the markers hisAl, metC3, and thr-5; and they are not alleles. SP/3 is a temperate bacteriophage of Bacillus subtilis (13). The normal prophage attachment site, attSP,8, is located between the ilvA and kauA genes (map position 1900) on the B. subtilis chromosome (7, 10, 15). Like phage X in Escherichia coli, SP,8 is capable of mediating

Study of chromosome rearrangements associated with the trpE26 mutation of Bacillus subtilis

“…Incidentally, the 1.3 kb segment, located at the Ia±Ib junction, probably represents the secondary SPb attach-ment site, previously localized in this region (Lipsky et al, 1981). SPb integration at this site would take place by a Campbell-type recombination event (Campbell, 1962), rather than by site-specific integration which occurs at the SPbatt site (for review, see Zahler, 1993).…”

Structure and organization of genes for transfer ribonucleic acid in Bacillus subtilis.

“…References are as follows: Ile, Ala, rrnO, and rrnA (37); rrnB, rrnC, rrnG-I (3); six-tRNA-gene cluster (sequenced published by Wawrousek and Hansen, 57; mapped by K. Bott, personal communication); 21-tRNA-gene cluster (sequence published by Green and Vold [16] and Wawrousek et al [59]; mapped by Stewart et al. [52]); sup-3 (20,31); sup44 (31). two suppressor mutations have been mapped (20, 31), both at position 30, (Fig.…”