Genetic Linkage Map of Olive Flounder, Paralichthys olivaceus

Abstract

Olive flounder, Paralichthys olivaceus, is an important fish species in Asia, both for fisheries and aquaculture. As the first step for better understanding the genomic structure and functional analysis, we constructed a genetic linkage map for olive flounder based on 180 microsatellites and 31 expressed sequence tag (EST)-derived markers. Twenty-four linkage groups were identified, consistent with the 24 chromosomes of this species. The total map distance was 1,001.3 cM based on Kosambi sex-average mapping, and the average inter-locus distance was 4.7 cM. Linkage between the loci was identified by an LOD score of ≥3. This linkage map may be used to map quantitative trait loci associated with important traits of the species and may assist in breeding programs.

Constructing a genetic linkage map and mapping quantitative trait loci for skeletal traits in Japanese flounder

“…Markers could be continually added to this map in the future studies and the DH line or their DNA can also be sent to other labs for additional analysis. Compared to the former ones, this genetic linkage map was constructed with 574 markers including 446 type II SSRs and 128 EST-SSRs, which makes it denser than the maps published before the year of 2008 (Coimbra et al 2003;Castaño-Sánchez et al 2008;Kang et al 2008), but not presently the densest flatfish linkage map. The second generation genetic linkage map of the Japanese flounder were constructed by 1375 markers (Castaño-Sánchez et al 2010).…”

“…Genetic linkage maps based on molecular markers at a large number of sites in the genome constitute an essential prerequisite to identify individual loci controlling targeted traits. In previous studies, the published Japanese flounder maps (Combria et al 2003;Kang et al 2008;Castaño-Sánchez et al 2010) have provided the framework for identifying a single major controlling the resistance to lymphocystis disease in hybrid family (Fuji et al 2006). A new linkage map of Japanese flounder constructed by Song et al (2012) and QTL associated with four growth traits were mapped.…”

Genomics and Genome Sequencing: Benefits for Finfish Aquaculture

“…In terms of other salmonids, linkage maps have been constructed for rainbow trout Nichols et al, 2003;Rexroad et al, 2005;Sakamoto et al, 2000), brown trout (Gharbi et al, 2006), coho salmon (Oncorhynchus kisutch) (McClelland & Naish, 2008), and Arctic charr . Other aquaculture species of interest for which linkage maps have been developed include catfish Waldbieser et al, 2001), Japanese flounder (Coimbra et al, 2003) European sea bass (Chistiakov et al, 2005), tilapia (Lee et al, 2005), common carp (Sun & Liang, 2004), olive flounder (Kang et al, 2008), Atlantic cod (Hubert et al, 2010;Moen et al, 2009b) and the list is expanding.…”

Microsatellite analysis as a tool for discriminating an interfamily hybrid between olive flounder and starry flounder

“…In total, 192 olive flounder microsatellite markers used in this study were taken from a previous study for the construction of an olive flounder linkage map (Kang et al, 2008). All 56 microsatellite markers for starry flounder were taken from the GenBank/EMBL/DDBJ database with the prefix Ksf.…”

“…They are also short in length, facilitate genotyping by polymerase chain reaction (PCR) and are codominant markers that allow the generation of maximum genetic information. Polymorphic microsatellite markers have been developed in both P. olivaceus and P. stellatus and have been applied to parentage relationships, monitoring populations, and linkage mapping of these fish (Jarne and Lagoda, 1996;Li et al, 2004;Kang et al, 2008).…”