Microsatellite analysis as a tool for discriminating an interfamily hybrid between olive flounder and starry flounder

Abstract

ABSTRACT. An interspecific artificial hybrid was produced between two economically important aquaculture flatfish: olive flounder (Paralichthys olivaceus) and starry flounder (P. stellatus). This hybrid displays the rapid growth characteristic of the former and tolerance to low temperatures and low salinity of the latter, but the genetics of inheritance in this hybrid have not been elucidated. Polymorphic microsatellite markers developed for P. olivaceus and P. stellatus were tested to determine if these markers can be used for analysis of parentage and genetic inheritance. Multiplex PCR using two primer sets that were specific to each species produced PCR products of different sizes; these could be used for the identification of interspecific hybrids. Among the 192 primers derived from olive flounder, 25.5% of the primer sets successfully amplified genomic DNA from starry flounder, and 23% of the 56 primer sets originating from starry flounder amplified DNA from olive flounder. Analysis of genetic inheritance in the hybrid using seven of the 62 microsatellite markers common to both species demonstrated classic Mendelian inheritance of these markers in the hybrid progeny, with the exception of one locus identified as a null allele in the hybrid. These results demonstrate that cross-specific microsatellite markers can be used tools for parentage analysis of hybrid flatfish, for mapping quantitative trait loci, for marker-assisted selective breeding, and for studies of the evolution of fish.

Rapid Development of Microsatellite Markers with 454 Pyrosequencing in a Vulnerable Fish, the Mottled Skate, Raja pulchra

“…To reduce the time and cost of MS marker development, pre-existing markers from related species have been applied to the study of many animal, fungus, plant and fish species [33,34]. Moreover, cross-species markers can be used for the identification of invading species and the identification of parental origin in hybrid fish [35]. Although the cross-species transferability of MS markers is unevenly distributed among taxa, over 40% and 25% of polymorphic marker transfers have been observed in fish of different genera within the same family, and of families within same order, respectively [33].…”

Wild and Hatchery Populations of Korean Starry Flounder (Platichthys stellatus) Compared Using Microsatellite DNA Markers

“…In conclusion, genetic studies on starry flounder with microsatellite DNA markers are very rare with only one recent report on the development of microsatellites as a tool for discriminating a hybrid between olive flounder and starry flounder [14]. No detailed information is available to date on the genetic diversity of wild and cultured stocks of starry flounder.…”

“…Microsatellites have been used to monitor genetic differences between hatchery stocks and wild populations in various fish species [11–13]. However, despite the importance of starry flounder for commercial aquaculture in Korea, there are only a limited number of MS markers available [14]. Furthermore, no study has focused on the genetic variability and population structure of this species.…”

QTL detection for Aeromonas salmonicida resistance related traits in turbot (Scophthalmus maximus)

“…In that study, the commercial strain RT-201 artificially challenged with IPN virus showed a mortality of 4.3%, whereas the highly sensitive controls reached 96.1%. In carps, Schäperclaus [6] found resistance to the dropsy disease, selected lines suffering low mortality (11.5%) well below the unselected ones (57%).…”