Genetic labeling reveals temporal and spatial expression pattern of D2 dopamine receptor in rat forebrain

Yu, Qing; Liu, Yingzi; Zhu, Yanbing; Wang, Yao-Yi; Li, Qian; Yin, Dong-Min

doi:10.1007/s00429-018-01824-2

Cited by 36 publications

(34 citation statements)

References 71 publications

(83 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…D2/3 receptors are also differentially expressed in PFCx and their activation contribute to specific cognitive processes ( Robinson and Sohal, 2017 ; Bailey et al., 2020 ; Papenberg et al., 2020 ). D2 are enriched within subcortically projecting L5 pyramidal neurons thick-tufted pyramidal cells, with projections to thalamus and pons, but not contralateral cortex ( Yu et al., 2019 ). These neurons exhibit a prominent hyperpolarization-activated cationic current.…”

Section: Section 2: Dr Alterations In Schizophreniamentioning

confidence: 99%

Dopamine Receptor Subtypes, Physiology and Pharmacology: New Ligands and Concepts in Schizophrenia

Martel¹,

McArthur²

2020

Front. Pharmacol.

169

125

View full text Add to dashboard Cite

Dopamine receptors are widely distributed within the brain where they play critical modulator roles on motor functions, motivation and drive, as well as cognition. The identification of five genes coding for different dopamine receptor subtypes, pharmacologically grouped as D1- (D1 and D5) or D2-like (D2S, D2L, D3, and D4) has allowed the demonstration of differential receptor function in specific neurocircuits. Recent observation on dopamine receptor signaling point at dopamine—glutamate-NMDA neurobiology as the most relevant in schizophrenia and for the development of new therapies. Progress in the chemistry of D1- and D2-like receptor ligands (agonists, antagonists, and partial agonists) has provided more selective compounds possibly able to target the dopamine receptors homo and heterodimers and address different schizophrenia symptoms. Moreover, an extensive evaluation of the functional effect of these agents on dopamine receptor coupling and intracellular signaling highlights important differences that could also result in highly differentiated clinical pharmacology. The review summarizes the recent advances in the field, addressing the relevance of emerging new targets in schizophrenia in particular in relation to the dopamine – glutamate NMDA systems interactions.

show abstract

Section: Section 2: Dr Alterations In Schizophreniamentioning

confidence: 99%

Dopamine Receptor Subtypes, Physiology and Pharmacology: New Ligands and Concepts in Schizophrenia

Martel¹,

McArthur²

2020

Front. Pharmacol.

169

125

View full text Add to dashboard Cite

show abstract

“…This indicates a functional role of dopamine in the entire OB, including the glomerular layer as well as in external plexiform layer, which is consistent with our results. The distribution of the D 2 receptor has its highest density in the GL and nerve layer, while D 2 receptor expression in the EPL is sparse 40,[43][44][45] . Despite that, the dopamine-induced calcium transients where strongly reduced in the presence of D 2 antagonist sulpiride in both the GL and EPL, indicating expression of D 2 receptors in astrocytes of the EPL.…”

Section: Scientific Reports |mentioning

confidence: 99%

Dopamine-induced calcium signaling in olfactory bulb astrocytes

Fischer

Scheffler

Lohr

2020

Sci Rep

View full text Add to dashboard Cite

ResultsDistribution of olfactory bulb astrocytes and tyrosine hydroxylase-expressing neurons. To analyze the distribution of OB astrocytes and TH-expressing neurons in the GL and EPL by immunohistochemistry, we used TH-Cre × tdTomato fl/fl mice and aimed to visualize astrocytes by anti-GFAP staining. GFAP-positive astrocytes were located throughout the GL, which can be distinguished from the ONL by glomerular structures, and the EPL, in which GFAP-expression was weaker compared to the GL (Fig. 1A). TH expression also exhibits its highest density in the GL, but the EPL also contains some TH-positive neurons and neuronal processes (Fig. 1A).In the glomerular layer, dendrites of tdTomato-positive SA cells are closely intermingled with astrocyte processes, suggesting a possible physiological interaction such as neurotransmitter release by SA cells and subsequently activation of astrocytic dopamine receptors (Fig. 1B,C). Close proximity of dendrites of TH-positive neurons and astrocyte processes could also be observed in the EPL, albeit at a lower density (Fig. 1D). In both layers, astrocyte processes were in direct vicinity of tdTomato-positive varicosities, presumptive dopamine release sites 18 . Scientific RepoRtS |(2020) 10:631 | https://doi.

show abstract

“…Surprisingly, despite the wealth of studies on the dopaminergic system, very little is known about D2R expression in the hippocampus proper (Gasbarri et al, 1997; Beaulieu and Gainetdinov, 2011); most studies to date, focused on the basal ganglia and occasionally on the frontal cortex (Levey et al, 1993; Yung et al, 1995; Santana et al, 2009; Lavian et al, 2018). The most extensive examination of D2R expression in rat hippocampus up to now was performed by Yu et al (2019). In their work they showed that D2R is mainly expressed in cells of the Stratum pyramidale (sp) and Stratum radiatum (sr) layers of the CA1–3 regions, in the dentate gyrus (DG), and in axon terminals innervating the Stratum lacunosum-moleculare (slm) of the CA1 of the dorsal hippocampus.…”

Section: Introductionmentioning

confidence: 99%

Gradient of Expression of Dopamine D2 Receptors Along the Dorso-Ventral Axis of the Hippocampus

Dubovyk

Manahan‐Vaughan

2019

Front. Synaptic Neurosci.

View full text Add to dashboard Cite

Dopamine D2-like receptors (D2R) play an important role in the regulation of hippocampal neuronal excitability and contribute to the regulation of synaptic plasticity, the encoding of hippocampus-dependent memories and the regulation of affective state. In line with this, D2R are targeted in the treatment of psychosis and affective disorders. It has been proposed that the dorso-ventral axis of the hippocampus can be functionally delineated into the dorsal pole that predominantly processes spatial information and the ventral pole that mainly addresses hippocampal processing of emotional and affective state. Although dopaminergic control of hippocampal information processing has been the focus of a multitude of studies, very little is known about the precise distribution of D2R both within anatomically defined sublayers of the hippocampus and along its dorsoventral axis, that could in turn yield insights as to the functional significance of this receptor in supporting hippocampal processing of spatial and affective information. Here, we used an immunohistochemical approach to precisely scrutinize the protein expression of D2R both within the cellular and dendritic layers of the hippocampal subfields, and along the dorso-ventral hippocampal axis. In general, we detected significantly higher levels of protein expression of D2R in the ventral, compared to the dorsal poles with regard to the CA1, CA2, CA3 and dentate gyrus (DG) regions. Effects were very consistent: the molecular layer, granule cell layer and polymorphic layer of the DG exhibited higher D2R levels in the ventral compared to dorsal hippocampus. D2R levels were also significantly higher in the ventral Stratum oriens, Stratum radiatum, and Stratum lacunosum-moleculare layers of the CA1 and CA3 regions. The apical dendrites of the ventral CA2 region also exhibited higher D2R expression compared to the dorsal pole. Taken together, our study suggests that the higher D2R expression levels of the ventral hippocampus may contribute to reported gradients in the degree of expression of synaptic plasticity along the dorso-ventral hippocampal axis, and may support behavioral information processing by the ventral hippocampus.

show abstract

Genetic labeling reveals temporal and spatial expression pattern of D2 dopamine receptor in rat forebrain

Cited by 36 publications

References 71 publications

Dopamine Receptor Subtypes, Physiology and Pharmacology: New Ligands and Concepts in Schizophrenia

Dopamine Receptor Subtypes, Physiology and Pharmacology: New Ligands and Concepts in Schizophrenia

Dopamine-induced calcium signaling in olfactory bulb astrocytes

Gradient of Expression of Dopamine D2 Receptors Along the Dorso-Ventral Axis of the Hippocampus

Contact Info

Product

Resources

About