Dopamine-induced calcium signaling in olfactory bulb astrocytes

Fischer, Timo; Scheffler, Paula; Lohr, Christian

doi:10.1038/s41598-020-57462-4

Cited by 27 publications

(29 citation statements)

References 60 publications

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“…In the absence of extracellular calcium, on the contrary, the norepinephrine-induced calcium transients were only slightly reduced in amplitude, but to a larger extend in area and duration, indicating an involvement of extracellular calcium at least in the late plateau-like phase of the calcium response. The most common mechanism to liberate calcium from the ER is the phospholipase C (PLC)/IP3 signaling pathway, which has previously been shown for olfactory bulb astrocytes to be activated by P2Y1, A2A, mGluR5, and dopamine receptors and has been confirmed in the present study (Doengi et al, 2008 ; Otsu et al, 2015 ; Fischer et al, 2020 ). Calcium withdrawal suppressed only the late plateau phase of the calcium response with little effect on the initial calcium peak, whereas suppression of internal calcium release by CPA entirely blocked norepinephrine-mediated calcium responses.…”

Section: Discussionsupporting

confidence: 86%

“…We used application of ADP to test the viability of the cells and to identify astrocytes in the olfactory bulb. Olfactory bulb astrocytes express P2Y1-receptors and respond to ADP with calcium transients, whereas earlier studies could show that Fluo-8-loaded olfactory bulb neurons do not respond to bath application of ADP (Doengi et al, 2008 , 2009 ; Fischer et al, 2020 ). The aim of our study was to find out whether norepinephrine induces calcium transients in olfactory bulb astrocytes.…”

Section: Resultsmentioning

confidence: 93%

“…To analyze changes of the calcium level in astrocytes, all cell somata were manually marked as regions of interests (ROIs), each ROI outlining the silhouette of the soma. Cells located in the GL and EPL that showed a calcium response to bath-applied ADP and to low extracellular K + were identified as astrocytes (Hartel et al, 2007 ; Doengi et al, 2008 ; Fischer et al, 2020 ). The astrocytic calcium elevations have been observed in both GL and EPL without a significant difference in amplitude or duration, therefore the results in the present study are pooled data of both layers.…”

Section: Methodsmentioning

confidence: 99%

“…However, norepinephrine is not the only neuromodulator that evokes calcium signaling in olfactory bulb astrocytes, yet it is the first "top down" neuromodulator released from centrifugal fibers described so far to act on astrocytes in the olfactory bulb. Dopamine is a neuromodulator that is released by local interneurons in the olfactory bulb and induced calcium responses in astrocytes similar to those evoked by norepinephrine (Fischer et al, 2020). In addition, ATP is released from nerve terminals of olfactory sensory neurons in the GL and is degraded by ectoenzymes to adenosine, another neuromodulator inducing calcium signaling in olfactory bulb astrocytes (Doengi et al, 2008;Thyssen et al, 2010;Thyssen et al, 2010).…”

Section: Astrocytes As Neuromodulatory Elements In the Olfactory Bulbmentioning

confidence: 99%

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Norepinephrine-Induced Calcium Signaling and Store-Operated Calcium Entry in Olfactory Bulb Astrocytes

Fischer

Prey

Eschholz

et al. 2021

Front. Cell. Neurosci.

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It is well-established that astrocytes respond to norepinephrine with cytosolic calcium rises in various brain areas, such as hippocampus or neocortex. However, less is known about the effect of norepinephrine on olfactory bulb astrocytes. In the present study, we used confocal calcium imaging and immunohistochemistry in mouse brain slices of the olfactory bulb, a brain region with a dense innervation of noradrenergic fibers, to investigate the calcium signaling evoked by norepinephrine in astrocytes. Our results show that application of norepinephrine leads to a cytosolic calcium rise in astrocytes which is independent of neuronal activity and mainly mediated by PLC/IP3-dependent internal calcium release. In addition, store-operated calcium entry (SOCE) contributes to the late phase of the response. Antagonists of both α1- and α2-adrenergic receptors, but not β-receptors, largely reduce the adrenergic calcium response, indicating that both α-receptor subtypes mediate norepinephrine-induced calcium transients in olfactory bulb astrocytes, whereas β-receptors do not contribute to the calcium transients.

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Section: Discussionsupporting

confidence: 86%

Section: Resultsmentioning

confidence: 93%

Section: Methodsmentioning

confidence: 99%

Section: Astrocytes As Neuromodulatory Elements In the Olfactory Bulbmentioning

confidence: 99%

See 2 more Smart Citations

Norepinephrine-Induced Calcium Signaling and Store-Operated Calcium Entry in Olfactory Bulb Astrocytes

Fischer

Prey

Eschholz

et al. 2021

Front. Cell. Neurosci.

Self Cite

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“…Lastly, how does dopamine affect glial cells, and how may this influence vision and olfaction? There is already evidence of OB astrocytes increasing their Ca 2+ activity in response to the activation of D 1 and D 2 Rs (Fischer et al, 2020), which is a fascinating note on which to end, because it implies that dopamine in the retina, OB, basal ganglia, and other brain areas would not only affect the neurons, but perhaps every type of brain cell.…”

Section: Resultsmentioning

confidence: 99%

Illuminating and Sniffing Out the Neuromodulatory Roles of Dopamine in the Retina and Olfactory Bulb

Korshunov

Blakemore

Trombley

2020

Front. Cell. Neurosci.

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In the central nervous system, dopamine is well-known as the neuromodulator that is involved with regulating reward, addiction, motivation, and fine motor control. Yet, decades of findings are revealing another crucial function of dopamine: modulating sensory systems. Dopamine is endogenous to subsets of neurons in the retina and olfactory bulb (OB), where it sharpens sensory processing of visual and olfactory information. For example, dopamine modulation allows the neural circuity in the retina to transition from processing dim light to daylight and the neural circuity in the OB to regulate odor discrimination and detection. Dopamine accomplishes these tasks through numerous, complex mechanisms in both neural structures. In this review, we provide an overview of the established and emerging research on these mechanisms and describe similarities and differences in dopamine expression and modulation of synaptic transmission in the retinas and OBs of various vertebrate organisms. This includes discussion of dopamine neurons' morphologies, potential identities, and biophysical properties along with their contributions to circadian rhythms and stimulus-driven synthesis, activation, and release of dopamine. As dysregulation of some of these mechanisms may occur in patients with Parkinson's disease, these symptoms are also discussed. The exploration and comparison of these two separate dopamine populations shows just how remarkably similar the retina and OB are, even though they are functionally distinct. It also shows that the modulatory properties of dopamine neurons are just as important to vision and olfaction as they are to motor coordination and neuropsychiatric/neurodegenerative conditions, thus, we hope this review encourages further research to elucidate these mechanisms.

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Dopamine plays a critical role in the olfactory adaptive learning pathway in Caenorhabditis elegans

Raj

Thekkuveettil

2022

J of Neuroscience Research

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Encoding and consolidating information through learning and memory is vital in adaptation and survival. Dopamine (DA) is a critical neurotransmitter that modulates behavior. However, the role of DA in learning and memory processes is not well defined.Herein, we used the olfactory adaptive learning paradigm in Caenorhabditis elegans to elucidate the role of DA in the memory pathway. Cat-2 mutant worms with low DA synthesis showed a significant reduction in chemotaxis index (CI) compared to the wild type (WT) after short-term conditioning. In dat-1::ICE worms, having degeneration of DA neurons, there was a significant reduction in adaptive learning and memory. When the worms were trained in the presence of exogenous DA (10 mM) instead of food, a substantial increase in CI value was observed. Furthermore, our results suggest that both dop-1 and dop-3 DA receptors are involved in memory retention. The release of DA during conditioning is essential to initiate the learning pathway. We also noted an enhanced cholinergic receptor activity in the absence of dopaminergic neurons. The strains expressing GCaMP6 in DA neurons (pdat-1::GCaMP-6::mCherry) showed a rise in intracellular calcium influx in the presence of the conditional stimulus after training, suggesting DA neurons are activated during memory recall. These results reveal the critical role of DA in adaptive learning and memory, indicating that DA neurons play a crucial role in the effective processing of cognitive function.

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Dopamine-induced calcium signaling in olfactory bulb astrocytes

Cited by 27 publications

References 60 publications

Norepinephrine-Induced Calcium Signaling and Store-Operated Calcium Entry in Olfactory Bulb Astrocytes

Norepinephrine-Induced Calcium Signaling and Store-Operated Calcium Entry in Olfactory Bulb Astrocytes

Illuminating and Sniffing Out the Neuromodulatory Roles of Dopamine in the Retina and Olfactory Bulb

Dopamine plays a critical role in the olfactory adaptive learning pathway in Caenorhabditis elegans

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