Genetic Identification of the Northeastern Atlantic Spiny Spider Crab as Maja Brachydactyla Balss, 1922

Sotelo, Graciela; Morán, Paloma; Posada, David

doi:10.1651/07-2875r.1

Cited by 31 publications

(27 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sequences amplified using universal primers were truncated to capture the interior portion amplified by taxon-specific primers. For 28S, we again used Primer3 software to construct majoid-specific interior primers using a partial 28S gene sequence from the Atlantic spider crab Maja squinado in GenBank (accession # DQ079799, Porter et al, 2005) (subsequent analysis by Sotelo et al, 2008 indicates this species is likely Maja brachydactyla). These primers (forward: 5 0 -GCAG TCTC TCAC CGCC TAAG TTAT G-3 0 ; reverse: 5 0 -GACT CCTT GGTC CGTG TTTC AAGA C-3 0 ) amplified a $620-bp portion of the 28S gene.…”

Section: Taxon Sampling and Molecular Markersmentioning

confidence: 99%

Molecular phylogeny of the brachyuran crab superfamily Majoidea indicates close congruence with trees based on larval morphology

Hultgren

Stachowicz

2008

Molecular Phylogenetics and Evolution

View full text Add to dashboard Cite

Section: Taxon Sampling and Molecular Markersmentioning

confidence: 99%

Molecular phylogeny of the brachyuran crab superfamily Majoidea indicates close congruence with trees based on larval morphology

Hultgren

Stachowicz

2008

Molecular Phylogenetics and Evolution

View full text Add to dashboard Cite

“…The spiny spider crab Maja brachydactyla Balss, 1922 is a decapod crustacean found in the northeastern Atlantic, from the British Isles to Senegal (Sotelo et al 2008). This crab has a complex life cycle that has been widely studied for the northwestern region of the Iberian Peninsula (Galicia) (Corgos 2004).…”

Section: Introductionmentioning

confidence: 99%

Genetic variation of the spiny spider crab Maja brachydactyla in the northeastern Atlantic

Sotelo

Morán²,

Fernández³

et al. 2008

Mar. Ecol. Prog. Ser.

View full text Add to dashboard Cite

The spiny spider crab Maja brachydactyla is a decapod crustacean that inhabits the northeastern Atlantic, from the British Isles to Senegal, and is the target of intense fishing activity. In the absence of previous genetic studies, we describe the genetic variability and structure of this species along Atlantic European coasts, paying special attention to the northwestern Iberian Peninsula (Galicia), where this species may be overexploited. Analysis of 2 mitochondrial fragments and 9 microsatellites indicates that these populations maintain appreciable levels of genetic diversity, most of which are found within localities. This scenario could be explained by quite large effective population sizes and noticeable levels of gene flow. M. brachydactyla in the northeastern Atlantic do not show any signs of a genetic bottleneck, and effectively conform to a diverse metapopulation with limited structure.

show abstract

“…However, species identification using the key morphological characters for adult Majidae of Neumann (1998) may be difficult for non-specialists to apply. Results of recent genetic analyses support the recognition of M. brachydactyla and M. squinado as different species (Sotelo et al, 2008(Sotelo et al, , 2009. M. brachydactyla has been reported in the eastern Atlantic, while M. squinado is restricted to the Mediterranean Sea (Neumann, 1998;d'Udekem d'Acoz, 1999;Sotelo et al, 2008Sotelo et al, , 2009.…”

Section: Introductionmentioning

confidence: 76%

“…Importantly, mtCOI is sufficiently variable to be useful in identifying and discriminating even the most closely related species (Lefébure et al, 2006;Bucklin et al, 2007Bucklin et al, , 2009Darling and Tepolt, 2008). Sotelo et al (2008) reported that the COI region displays diagnostic differences between species of the genus Maja; divergence between species was much higher than within them. The low level of intraspecific variation observed among the three species indicates that the COI gene fragment amplified utilizing the primers of Folmer et al (1994) is a suitable marker for identifying Majidae species in the NE Atlantic and Mediterranean Sea.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Identification of European species of <i>Maja</i> (Decapoda: Brachyura: Majidae): RFLP analyses of COI mtDNA and morphological considerations

Guerao¹,

Andrée²,

Froglia

et al. 2011

Sci. Mar.

View full text Add to dashboard Cite

SUMMARY: Four species of crabs of the genus Maja have been described along the European coast: M. brachydactyla, M. squinado, M. goltziana and M. crispata. The commercially important species M. brachydactyla and M. squinado achieve the largest body sizes and are the most similar in morphology, and are therefore easily confused. The four species of Maja were identified using a novel morphometric index and a polymerase chain reaction followed by restriction fragment length polymorphism analysis (RFLP). The relationship between carapace length and the distance between the tips of antorbital spines was used to distinguish adults of M. brachydactyla and M. squinado. PCR-RFLP analysis of a partial sequence of the mitochondrial cytochrome oxidase type I (COI) revealed that the four species of the genus Maja can be unambiguously discriminated using the combination of restriction endonucleases enzymes HpyCH4V and Ase I. The molecular identification may be particularly useful in larvae, juvenile and young crabs, when the morphological differences found in adults are not applicable.Keywords: Maja, M. squinado, M. brachydactyla, molecular identification, morfometry, COI, RFLP. RESUMEN: Identificación de las especies europeas del género Maja (Decapoda: Brachyura: Majidae): análisis de PCR-RFLP de una región del mtADN COI y consideraciones morfológicas. -Cuatro especies del género Maja han sido descritas en las costas europeas: M. brachydactyla, M. squinado, M. goltziana y M. crispata. Las especies M. brachydactyla y M. squinado, que tienen importancia comercial, alcanzan los tamaños más grandes y son morfológicamente muy similares, siendo muy fácil confundirlas. La identificación de las cuatro especies se ha realizado utilizando un nuevo índice morfométrico y un análisis de polimorfismos de fragmentos de restricción (RFLP). La relación entre la longitud del cefalotórax y la distancia entre los extremos distales de las espinas antorbitales se ha utilizado para la diferenciación de los adultos de M. brachydactyla y M. squinado. El análisis PCR-RFLP de una secuencia parcial de la citocromo oxidasa tipo I mitocondrial (COI) indica que las cuatro especies del género Maja pueden ser discriminadas usando una combinación de las endonucleasas HpyCH4V y Ase I. La identificación molecular puede ser particularmente útil en las larvas, juveniles y cangrejos jóvenes, cuando las diferencias morfológicas encontradas en los adultos no son aplicables.Palabras clave: Maja, M. squinado, M. brachydactyla, identificación molecular, morfometría, COI, RFLP.

show abstract

Genetic Identification of the Northeastern Atlantic Spiny Spider Crab as Maja Brachydactyla Balss, 1922

Cited by 31 publications

References 27 publications

Molecular phylogeny of the brachyuran crab superfamily Majoidea indicates close congruence with trees based on larval morphology

Molecular phylogeny of the brachyuran crab superfamily Majoidea indicates close congruence with trees based on larval morphology

Genetic variation of the spiny spider crab Maja brachydactyla in the northeastern Atlantic

Identification of European species of <i>Maja</i> (Decapoda: Brachyura: Majidae): RFLP analyses of COI mtDNA and morphological considerations

Contact Info

Product

Resources

About