Genetic Identification of POU1F1|PstI gene of Lakor Goat from Lakor Island, Southwest Maluku Regency

Abstract

Lakor goat is a domestic animal from Lakor island in South West Maluku Regency. They successfully survive and reproduce with limited water, grass and high temperature. Growth traits is an economic trait which important for breeding strategy (productivity improvement). Pituitary Specific Transcription Factor 1 (POU1F1) is otherwise known as PIT1 gene plays a role of growth and carcass traits. The objective of this study was to identify the POU1F1 gene in Lakor goat from Lakor island in Southwest Maluku Regency. Sixty-three samples of hair tail were collected from three different locations: Ketti Letpey (18), Werwawan Jamluli (26) and Letoda (19). DNA was extracted by DNA isolation kit. POU1FI gene was amplified using PCR technique while genotyping used PCR-RFLP with PstI restriction enzyme. Result showed that only one genotype (TT) was found and monomorphic. It may be caused remote area and limited of ram that indicated inbreeding has occurred. Inbreeding rate or coefficient study and introgression of ram from out of Lakor island by Artificial Insemination (AI) or natural mating is needed.

Genetic characterization and phylogenetic study of Lakor goat from Southwest Maluku Regency based on mitochondrial COI gene

“…Cox and Hebert, Wares and Cunningham [ 26 , 27 ] demonstrated that the COI gene has a rapid evolution rate to allow discrimination of not only closely associated species but also phylogeographic groups within a single species. In some cases, traditional (conventional) human selection of certain goat breeds slowly results in extreme size differences and unique adaptive mechanisms to manage environmental stresses as illustrated with Lakor goats [ 28 ] and large-sized Shire horses [ 29 ]. This has an impact on the occurrence of inbreeding depression within natural populations.…”

“…According to Volkandari et al . [ 28 ], there is low genetic variation within the Lakor goat population after few generations of selection and domestication. This condition is due to improper breeding mechanisms and broodstock management.…”

Polymorphism of Growth Hormone (GH) Gene in Lakor Goat from Lakor Island of Southwest Maluku Regency

“…Research by Volkandari et al (2019) and Rumanta et al (2020) reported that there is low genetic variation within the Lakor goat population after few generations of selection and domestication. This condition is due to improper breeding mechanisms and broodstock management.…”

“…Goat is one of the livestock of the principal meat producing animals in Indonesia and has immense contribution to the resource poor section of society for their livelihood. Several goat breeds that have adapted to the environment and geography in the territory of Indonesia, they are Marica, Bengal, Etawah, Kosta, Kacang, Gembrong, Muara, Samosir (Batubara et al, 2013), and Lakor goats (Volkandari et al 2019;Rumanta et al 2020). Budisatria et al (2008), stated that goats are known as livestock that produce meat, milk, and fur, but are also used in traditional and religious ceremonies.…”

“…Based on morphological characters, color of Lakor goat is varies from white, black, brown, grey, crème, and combination of those five colors. Volkandari et al (2019), reported that body color of Lakor goat has a combination of solid color with black and white, grey, brown, and spot black white while head color is dominant of black. Growth hormone (GH) plays a vital role in different biological processes, such as growth, metabolism, lactation, and reproduction of livestock species (Katoh et al 2008;Seevagan et al 2015).…”