Genetic identification of a newly synthetic allopolyploid strain with 206 chromosomes in polyploid gibel carp

Lu, Meng; Wang, Zhongwei; Hu, Chongjiang; Zhou, Li; Gui, Jian‐Fang

doi:10.1111/are.13485

Cited by 12 publications

(14 citation statements)

References 33 publications

(57 reference statements)

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“…On the other hand, although the dominant phyla in BL and BH group were Proteobacteria, Firmicutes and Cyanobacteria, while their relative abundance of the dominant phyla was significant difference, which indicated that the same fish (Beluga sturgeon) fed different diets were able to affect the intestinal microbiota. Lv et al [23] found that the relative abundances of intestinal microbiota were difference between the wild Kaluga ( Huso dauricus ) and cultured Kaluga sturgeon, and their predominant bacteria are Proteobacteria and Fusobacteria, respectively. Besides, there were reported that the microbiota composition of Atlantic Salmon ( Salmo salar ) was influenced by experimental diets when they fed with fishmeal, soybean meal or fermented soybean meal diets [30].…”

Section: Discussionmentioning

confidence: 99%

Comparative study on the effects of different feeding habits and diets on intestinal microbiota in Acipenser baeri Brandt and Huso huso

Wang

et al. 2019

BMC Microbiol

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BackgroundSiberian sturgeon (Acipenser baeri Brandt) and Beluga sturgeon (Huso huso) are two important commercial fish in China, and the feeding habits of them are very different. Diets and feeding habits are two significant factors to affect the gastrointestinal microbiota in fish. The intestinal microbiota has been reported to play a key role in nutrition and immunity. However, it is rarely reported about the relationship between the intestinal microbiota and feeding habits/diets on different Acipenseridae fish. This study is to comparative analysis of gut microbial community in Siberian sturgeon and Beluga sturgeon fed with the same diet/Beluga sturgeon fed with different diets in order to determine the effects of different feeding habits/diets on the fish intestinal microbiota.ResultsAccording to the experimental objectives, BL and BH groups were Beluga sturgeon (Huso huso) fed with low fishmeal diet and high fishmeal diet, respectively. SH group represented Siberian sturgeon (Acipenser baeri Brandt) fed with the same diet as BH group. After 16 weeks feeding trial, the intestinal microbiota was examined by 16S rRNA high-throughput sequencing technology. On the phylum level, Proteobacteria and Bacteroidetes were significantly higher in BL group than BH group, and Cyanobacteria showed the opposite trend. Compared with BH group, Proteobacteria and Firmicutes were significantly increased in SH group, whereas Cyanobacteria were clearly decreased. At the genus level, Pseudomonas and Citrobacter in BL group were significantly higher comparing with BH group, while Bacillus, Luteibacter, Staphylococcus and Oceanobacillus was lower in BH group than SH group.ConclusionsAlpha and beta diversities indicated that the intestinal microflora were significant difference between Siberian sturgeon and Beluga sturgeon when they fed with the same diet. Meanwhile, Beluga sturgeon fed with low fishmeal diet can increase the species diversity of intestinal microbiota than it fed high fishmeal diet. Therefore, feeding habits clearly affected the gastrointestinal microbiota of sturgeons. Moreover, the impact of changes in food on the gut microbiota of sturgeons should be taken into consideration during the process of sturgeon aquaculture.

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Section: Discussionmentioning

confidence: 99%

Comparative study on the effects of different feeding habits and diets on intestinal microbiota in Acipenser baeri Brandt and Huso huso

Wang

et al. 2019

BMC Microbiol

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“…Cell culture, colchicine treatment and chromosome preparation were the same as described (Liu, 2015). Two kinds of DNA probes were used to perform FISH analysis according to standard procedures as described previously (Lu et al, 2018). The fragments specific to sex chromosome B22 of C. auratus amplified by PCR (Tables S6 and S7 in Supporting Information) were labeled by DIG-Nick Translation Mix (Roche, Switzerland), and the green fluorescent signals were obtained with fluorescein isothiocyanate (FITC) conjugated-DIG antibody (Roche, Switzerland).…”

Section: Chromosome Preparation and Fluorescence In Situ Hybridization (Fish)mentioning

confidence: 99%

Regain of sex determination system and sexual reproduction ability in a synthetic octoploid male fish

et al. 2020

Sci. China Life Sci.

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Polyploids in vertebrates are generally associated with unisexual reproduction, but the direct consequences of polyploidy on sex determination system and reproduction mode remain unknown. Here, we synthesized a group of artificial octoploids between unisexual gynogenetic hexaploid Carassius gibelio and sexual tetraploid Carassius auratus. The synthetic octoploids were revealed to have more than 200 chromosomes, in which 50 chromosomes including the X/Y sex determination system were identified to transfer from sexual tetraploid C. auratus into the unisexual gynogenetic hexaploid C. gibelio. Significantly, a few synthetic octoploid males were found to be fertile, and one octoploid male was confirmed to regain sexual reproduction ability, which exhibits characteristics that are the same to sexual reproduction tetraploid males, such as 1:1 sex ratio occurrence, meiosis completion and euploid sperm formation in spermatogenesis, as well as normal embryo development and gene expression pattern during embryogenesis. Therefore, the current finding provides a unique case to explore the effect of sex determination system incorporation on reproduction mode transition from unisexual gynogenesis to sexual reproduction along with genome synthesis of recurrent polyploidy in vertebrates.

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“…gibelio × C. carpio ( Gui et al. 1993a ; Lu et al. 2018 ), which might arise by the integration of C a .…”

Section: Discussionmentioning

confidence: 99%

“…1993a , 1993b ; Li et al. 2016 ; Lu et al. 2018 ), and some of them have been applied in aquaculture practice owning to their growth superiority ( Li et al.…”

Section: Introductionmentioning

confidence: 99%

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Whole Genome Incorporation and Epigenetic Stability in a Newly Synthetic Allopolyploid of Gynogenetic Gibel Carp

Shao

Wang

et al. 2018

Genome Biology and Evolution

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Allopolyploidization plays an important role in speciation, and some natural or synthetic allopolyploid fishes have been extensively applied to aquaculture. Although genetic and epigenetic inheritance and variation associated with plant allopolyploids have been well documented, the relative research in allopolyploid animals is scarce. In this study, the genome constitution and DNA methylation inheritance in a newly synthetic allopolyploid of gynogenetic gibel carp were analyzed. The incorporation of a whole genome of paternal common carp sperm in the allopolyploid was confirmed by genomic in situ hybridization, chromosome localization of 45S rDNAs, and sequence comparison. Pooled sample-based methylation sensitive amplified polymorphism (MSAP) revealed that an overwhelming majority (98.82%) of cytosine methylation patterns in the allopolyploid were inherited from its parents of hexaploid gibel carp clone D and common carp. Compared to its parents, 11 DNA fragments in the allopolyploid were proved to be caused by interindividual variation, recombination, deletion, and mutation through individual sample-based MSAP and sequencing. Contrast to the rapid and remarkable epigenetic changes in most of analyzed neopolyploids, no cytosine methylation variation was detected in the gynogenetic allopolyploid. Therefore, the newly synthetic allopolyploid of gynogenetic gibel carp combined genomes from its parents and maintained genetic and epigenetic stability after its formation and subsequently seven successive gynogenetic generations. Our current results provide a paradigm for recurrent polyploidy consequences in the gynogenetic allopolyploid animals.

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Genetic identification of a newly synthetic allopolyploid strain with 206 chromosomes in polyploid gibel carp

Cited by 12 publications

References 33 publications

Comparative study on the effects of different feeding habits and diets on intestinal microbiota in Acipenser baeri Brandt and Huso huso

Comparative study on the effects of different feeding habits and diets on intestinal microbiota in Acipenser baeri Brandt and Huso huso

Regain of sex determination system and sexual reproduction ability in a synthetic octoploid male fish

Whole Genome Incorporation and Epigenetic Stability in a Newly Synthetic Allopolyploid of Gynogenetic Gibel Carp

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