Comparative study on the effects of different feeding habits and diets on intestinal microbiota in Acipenser baeri Brandt and Huso huso

Abstract

BackgroundSiberian sturgeon (Acipenser baeri Brandt) and Beluga sturgeon (Huso huso) are two important commercial fish in China, and the feeding habits of them are very different. Diets and feeding habits are two significant factors to affect the gastrointestinal microbiota in fish. The intestinal microbiota has been reported to play a key role in nutrition and immunity. However, it is rarely reported about the relationship between the intestinal microbiota and feeding habits/diets on different Acipenseridae fish. This study is to comparative analysis of gut microbial community in Siberian sturgeon and Beluga sturgeon fed with the same diet/Beluga sturgeon fed with different diets in order to determine the effects of different feeding habits/diets on the fish intestinal microbiota.ResultsAccording to the experimental objectives, BL and BH groups were Beluga sturgeon (Huso huso) fed with low fishmeal diet and high fishmeal diet, respectively. SH group represented Siberian sturgeon (Acipenser baeri Brandt) fed with the same diet as BH group. After 16 weeks feeding trial, the intestinal microbiota was examined by 16S rRNA high-throughput sequencing technology. On the phylum level, Proteobacteria and Bacteroidetes were significantly higher in BL group than BH group, and Cyanobacteria showed the opposite trend. Compared with BH group, Proteobacteria and Firmicutes were significantly increased in SH group, whereas Cyanobacteria were clearly decreased. At the genus level, Pseudomonas and Citrobacter in BL group were significantly higher comparing with BH group, while Bacillus, Luteibacter, Staphylococcus and Oceanobacillus was lower in BH group than SH group.ConclusionsAlpha and beta diversities indicated that the intestinal microflora were significant difference between Siberian sturgeon and Beluga sturgeon when they fed with the same diet. Meanwhile, Beluga sturgeon fed with low fishmeal diet can increase the species diversity of intestinal microbiota than it fed high fishmeal diet. Therefore, feeding habits clearly affected the gastrointestinal microbiota of sturgeons. Moreover, the impact of changes in food on the gut microbiota of sturgeons should be taken into consideration during the process of sturgeon aquaculture.

Effects of Dietary Proanthocyanidins Supplementation on Growth Performance, Digestive Enzymes Activities and Microbiota in the Intestine of Juvenile American Eels (Anguilla rostrata) Cultured in Cement Tanks

“…The intestinal microbiota plays an important role in fish health by providing vital nutrients and stimulating the innate immune response, the balance of bacteria communities in the intestine for the natural resistance of fish (Abdelhamed et al, 2019). The alpha diversity is a reflection of richness and diversity indices of the bacterial communities, including indexes of Chao1, ACE, Shannon and Simpson (Xu et al, 2019). In the present study, PACs group showed higher values of Chao1 and ACE index compared with control group, demonstrated that dietary PACs could improve the richness of the intestinal microbiota of American eel.…”

A Tentative Study of the Effects of Heat-Inactivation of the Probiotic Strain Shewanella putrefaciens Pdp11 on Senegalese Sole (Solea senegalensis) Intestinal Microbiota and Immune Response

“…Furthermore, a common phylum in marine fish is Proteobacteria [ 69 ], standing out for participating in various biogeochemical processes (carbon, nitrogen, and sulfur cycling) in aquatic ecosystems [ 70 ]. Here, this phylum is present in the intestinal microbiota of fish receiving all diets and is mostly represented by genera including Pseudomonas , Stenotrophomonas , Luteibacter , and Vibrio .…”

Effects of Short Chain Fatty Acids (SCFAs) Modulation on Potentially Diarrhea Causing Pathogens in Yaks Through Metagenomics Sequencing

“…The well-known complex intestinal tract is colonized by a large and diverse type of microbial microbiota [10]. This community produces extensive amount of metabolic products in intestine, which interact intimately with host cells to maintain physiological processes and functions i.e.…”

“…This community produces extensive amount of metabolic products in intestine, which interact intimately with host cells to maintain physiological processes and functions i.e. nutrition absorption, host metabolism, and immunity [10][11][12]. Mainly, microbiota bene ts the host through intestinal epithelium and by producing bene cial metabolites, that helps in food digestion and also against the pathogenic invasion.…”