Genetic Identification and Conservation of Local Turkish Grapevine (Vitis vinifera L.) Genotypes on the Edge of Extinction

Sabır, Ali; Ikten, Hatice; Mutlu, Nedim; Sarı, Duygu

doi:10.1007/s10341-017-0335-9

Cited by 8 publications

(3 citation statements)

References 26 publications

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“…These markers have some relevant advantages for research such as their highly informative, co-dominant, multi-allele genetic markers and high levels of polymorphism (Cipriani et al, 2010;Emanuelli et al, 2013). The efficiency of microsatellite markers in determining genetic relationships among various genotypes of grapevine and identifying synonym or homonym genotypes has already been verified previous works (Sabir et al, 2017), and identification of these grapes is of great importance in the conservation and management of genetic resources. The genetic diversity of wild and cultivated grapevine germplasm from the Mediterranean Basin and Central Asia was extensively investigated by a set of 20 nuclear SSR markers (Riaz et al, 2018).…”

Section: Introductionmentioning

confidence: 95%

“…All those statistics were calculated using GenAIEx software version 6.2 (Peakall and Smouse, 2006). To determine the similarity between the studied accessions, using the Numerical Taxonomy and Multiware Analysis System (NTSYS-pc) version 2.2 (Rohlf, 2006), cluster analysis was performed through UPGMA algorithm based on Jaccard's similarity coefficient (Jaccard, 1908). Jaccard's similarity coefficients was calculated using the SIMQUAL program in NTSYS-pc software, which is the common estimator of genetic identity and was calculated as follows: JC = N AB /(N AB + N A + N B ), where N AB is the number of marker bands shared by two samples (A and B), and N A and N B represent the marker bands present only in sample A or B, respectively.…”

Section: Analysis Of Nuclear Microsatellite Datamentioning

confidence: 99%

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Microsatellite Markers Analysis for the Genetic Characterization and Relationships among Some of Iranian Local Grapevine Accessions (Vitis ViniferaL.)

Taheri

Ramandi

2020

International Journal of Fruit Science

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One of the most important requirements for cultivar identification in Vitis species is rapid and consistent genotyping using DNA-based markers. Genetic characterization and diversity of grapevine cultivars using molecular markers have been the subject of many studies. The Iranian grapevine biodiversity is one of the most important in the Middle East. In the present work, fourteen nuclear microsatellite markers were used to assess the genetic diversity and relationships among 25 local grapevine accessions collected from vine-growing areas of Qazvin province (Iran). A total of 81 distinct alleles were produced with amplified product sizes ranging from 91 to 432 bp. The number of observed alleles for each locus ranged from 2 (ISV3) to 9 (VVS3 and VVMD6), with the overall average of 5.79 allele per locus. The observed heterozygosity (Ho) ranged from 0.53 (ISV3) to 0.72 (VVSMD25), while the expected heterozygosity (He) varied between 0.49 (ISV3) and 0.87 (VVSMD25). The polymorphism information content (PIC) ranged between 0.50 for VVS3 and ISV3 to 0.87 for VVSMD25. However, Shannon's information index (I) was found to be highest (2.13) in VVSMD25 locus, while the VVS3 locus had the lowest value with an average of 0.75 among SSR markers. According to Jaccard's similarity coefficient and UPGMA algorithm, cluster analysis assigned the accessions into five groups. Despite similar names, the results revealed that the accessions belonged to different groups. The obtained PCoA scatter plots further strongly supported their UPGMA dendrogram results. Beside VVMD, VVS series of markers were also used in the present study and the results showed that these series of microsatellite markers were as efficient as VVMD series in terms of discrimination and polymorphism for studying grapevine cultivars. Among all the microsatellite loci, VVSMD25 (PID = 0.03, PIC = 0.87) was found to be the most informative among the accessions.

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Section: Introductionmentioning

confidence: 95%

Section: Analysis Of Nuclear Microsatellite Datamentioning

confidence: 99%

Microsatellite Markers Analysis for the Genetic Characterization and Relationships among Some of Iranian Local Grapevine Accessions (Vitis ViniferaL.)

Taheri

Ramandi

2020

International Journal of Fruit Science

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“…Thousands of cultivars classified as Vitis vinifera L. subsp. Sativa are widely cultivated for fresh fruit, raisin (dried grape berries), juice and mainly for wine [1,2]. Domestication of the grapevine appears to have occurred between the seventh and the fourth millennia BC, in an area between the Black Sea and Iran [3,4].…”

Section: Introductionmentioning

confidence: 99%

Sustainable Viticulture Practices on the Face of Climate Change

Sabır

2018

ARTOAJ

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Change in past climate is evidenced to alter regional temperature and precipitation, resulting in non-neglectable influences on agricultural activities and crop production. In viticulture, climate change can cause multiple co-occurring changes in cultural conditions, growing seasons, environmental conditions, winter frost, vine phenology, grape berry composition, biotic disturbance, and abiotic disturbance. In order to alleviate the negative effects of climate change, the use of adapted genotypes is a better tool as it has the advantage of being environmentally friendly and cost effective. Besides, many other cultural practices should be employed simultaneously to obtain better results.

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Changes in Physical and Biochemical Features of Grape (Vitis vinifera L. cv ‘Prima’) Seeds as Influenced by Sustained Deficit Irrigation under Different Rootstock Effect

et al. 2020

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Genetic Identification and Conservation of Local Turkish Grapevine (Vitis vinifera L.) Genotypes on the Edge of Extinction

Cited by 8 publications

References 26 publications

Microsatellite Markers Analysis for the Genetic Characterization and Relationships among Some of Iranian Local Grapevine Accessions (Vitis ViniferaL.)

Microsatellite Markers Analysis for the Genetic Characterization and Relationships among Some of Iranian Local Grapevine Accessions (Vitis ViniferaL.)

Sustainable Viticulture Practices on the Face of Climate Change

Changes in Physical and Biochemical Features of Grape (Vitis vinifera L. cv ‘Prima’) Seeds as Influenced by Sustained Deficit Irrigation under Different Rootstock Effect

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