Genetic Features of Reproductive Traits in Bovine and Buffalo: Lessons From Bovine to Buffalo

Shao, Bin; Sun, Hui; Ahmad, Muhammad Jamil; Ghanem, Nasser; Abdel-Shafy, Hamdy; Du, Chao; Deng, Tingxian; Mansoor, Shahid; Zhou, Yang; Yang, Yifen; Zhang, Shujun; Yang, Liguo; Hua, Guohua

doi:10.3389/fgene.2021.617128

Cited by 27 publications

(15 citation statements)

References 127 publications

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“…Improving fertility is critical for lowering production costs, where the demand for buffalo's meat is increasing recently due to over-growing population. During selection indexes, the reproductive traits including ovulation, mating and calving-related traits were depreciated to increase livestock genetic capacity [16].…”

Section: Resultsmentioning

confidence: 99%

A Study on the Transcriptional Profile of NOS2 and IFN-γ Genes in River Buffalo with Endometritis

Othman

Osman

El-Maaty

et al. 2021

BJI

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Background and Aim: Uterine lumen contamination with bacteria is ubiquitous in buffalo after parturition. Nearly one-third of these infected animals develop endometritis which leads to reduced fertility. The present study aimed to evaluate the expressions of IFN-γ and NOS2 genes in uterine tissue of buffaloes with endometritis and comparing them with those in healthy animals using RT-qPCR Materials and Methods: Uterine samples were collected from 50 apparently healthy and 50 clinically infected buffaloes. RNA was extracted from the collected buffalo's uteri and cDNA was synthesized from extracted RNA. Quantitative Real Time PCR technique was performed using this synthesized cDNA. Results: Apparent up-regulation of both genes mRNA expression was recorded in endometritis-infected animals with 8.3-folds for IFN-γ and 9.99-folds for NOS2 (P<0.001). Conclusion: The upregulation of IFN-γ and NOS2 expression in the uterine tissue of endometritis-infected buffaloes can be used as a scale for measuring the efficiency of drugs used for endometritis treatment.

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Section: Resultsmentioning

confidence: 99%

A Study on the Transcriptional Profile of NOS2 and IFN-γ Genes in River Buffalo with Endometritis

Othman

Osman

El-Maaty

et al. 2021

BJI

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“…MTNR1A gene's polymorphisms can influence the protein through the impact on the amount of melatonin that affects the amount of prolactin (35). So, the MTNR1A gene's polymorphisms can be considered as a reproductive genetic marker (36,37), and therefor this genetic variation can effectively contribute to the reproductive performance of the Iraqi buffalo (38).…”

Section: Discussionmentioning

confidence: 99%

Molecular description of melatonin receptor 1A gene in Iraqi buffalo

Habib¹,

Al-Rishdy²,

AL-Hellou³

2022

IJVS

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The water buffalo has a seasonal reproductive pattern with reduced sexual activity during the longer photoperiod. The goal of this study was to identify the single nucleotide polymorphism (SNP) of melatonin receptor 1A (MTNR1A) gene in Iraqi buffalo cows and 3D structure of its protein and phylogeny with other sequences around the world. The 824 bp fragment of exon II of the MTNR1 A gene was amplified from 190 buffalo cows (4-5 years old) genomic DNA belonging to local breeders in Al-Chibayish Marshes, Southern Iraq. Amplified PCR products underwent custom sequencing at the two ends (5′ and 3′ ends). Five separate polymorphism sites, the 1st included 52 animals with 19 mutations (12 missense), the 2nd included 39 animals with 18 mutations (11 missense), the 3rd included 35 animals with 18 mutations (12 missense), the 4th included 32 animals with 18 mutations (12 missense) and the 5th included 32 animals with 14 mutations (8 missense). These polymorphic sites with accession numbers LC565046, LC565047, LC565709, LC565710 and LC565711 respectively were registered in gene bank. The phylogenetic tree reveals that in some of the Iraqi buffalo, the sequences of MTNR1A gene has identical to the Italian buffalo (GU817415), and the Brazilian buffalo (JN689386). Data revealed a marked difference in the fifth polymorphism sites' 3D protein structure because of the mutations. In conclusion, as a result of mutations, the gene MTNR1A in Iraqi buffalo has polymorphisms; these polymorphisms may be linked to gene function, Therefore, further studies are needed to connect the polymorphisms of this gene with the productive and reproductive traits.

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“…Reproductive features, particularly in monotocous livestock such as cattle and buffalo, are economically significant for long-term food production [ 1 ]. An extended period between two calvings (repeat breeding), might be regarded as low reproductive capacity or infertility.…”

Section: Introductionmentioning

confidence: 99%

Genetic structure of some candidate genes of repeat breeder syndrome in Egyptian buffaloes

Mahrous

Kader

Abdelhafez

et al. 2022

Journal of Genetic Engineering and Biotechnology

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Background This study aimed to explore the association between polymorphisms in three genes: leptin (LEP), leptin receptor (LEPR), and BMP4, and incidence of repeat breeding in Egyptian buffaloes. Methods DNA was extracted from 160 female buffaloes, involving 108 fertile and 52 repeat breeders. Genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Sequence analysis and alignment were performed by employing NCBI/BLAST/blastn suite, to identify SNPs among different patterns and alleles. We utilized PredictSNP software to predict the non-synonymous SNPs influences on protein function. Moreover, the conservation score of the amino acids within the target proteins was computed by ConSurf server. Results The genotyping results showed that LEP and BMP4 genes were monomorphic (CC, GG) in all tested fertile and repeat breeder buffaloes. Leptin gene sequencing showed a non-synonymous C73T SNP, replacing R to C at position 25 within the leptin polypeptide (position 4 in the mature form; R4C) which is a neutral mutation, not affecting function or structure of LEP protein. For LEPR, one synonymous SNP (T102C) and two non-synonymous SNPs (A106G and C146A), triggering V967A and G954C replacements, respectively in LEPR protein. Moreover, they are neutral mutations. Sequencing results of BMP4 showed HinfI restriction site indicate fixed GG genotype (CC genotype in the anti-sense strand) in all sequenced samples. No SNPs were observed within the amplified region. Conclusion Genotyping and sequencing results of the surveyed three genes revealed that there is no association between these genes mutations and the incidence of repeat breeding in Egyptian buffaloes.

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Genetic Features of Reproductive Traits in Bovine and Buffalo: Lessons From Bovine to Buffalo

Cited by 27 publications

References 127 publications

A Study on the Transcriptional Profile of NOS2 and IFN-γ Genes in River Buffalo with Endometritis

A Study on the Transcriptional Profile of NOS2 and IFN-γ Genes in River Buffalo with Endometritis

Molecular description of melatonin receptor 1A gene in Iraqi buffalo

Genetic structure of some candidate genes of repeat breeder syndrome in Egyptian buffaloes

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