Genetic evidence for a chromosomally integrated multiresistance plasmid in Salmonella dublin

Woodward, Martin J.; McLaren, I.; Wray, C.

doi:10.1099/00222615-28-3-205

Cited by 9 publications

(4 citation statements)

References 17 publications

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“…Since resistance to ACGKSSuTm had been retained and there had been no change in phage type, it is probable that the ACGKSSuTm resistance genes and the typing phage restriction genes had become chromosomally integrated. Chromosomal integration of plasmid-mediated multiple drug resistance has been reported in S. dublin isolated in Germany and Britain [24,25]; the integration of plasmid-mediated drug resistance genes into the S. typhimurium chromosome, as reported here, could be another example of the ability of the bacterial cell to conserve plasmid-mediated antimicrobial resistance genes in the absence of selective pressure.…”

Section: Plasmid Characterizationsupporting

confidence: 57%

Instability of multiple drug resistance plasmids inSalmonella typhimuriumisolated from poultry

1991

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SUMMARYPlasmids in five strains of Salmonella typhimurium resistant to ampicillin, chloramphenicol, gentamicin, neomycin/kanamycin, streptomycin, sulphonamides, tetracyclines and trimethoprim (ACGKSSuTTm), CGKSSuTTm, ACSSuT or CSSuT which had been isolated from poultry in the first 3 months of 1989 have been characterized and compared with plasmids in two strains of R-types ACGKSSuTTm and ASSuTTm isolated from two patients later in the year. With the exception of the human isolate of R-type ASSuTTm, all strains carried two non-conjugative plasmids, one coding for SSu and belonging to incompatibility group Q, and a second coding for multiple resistance and belonging to the Flme

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Section: Plasmid Characterizationsupporting

confidence: 57%

Instability of multiple drug resistance plasmids inSalmonella typhimuriumisolated from poultry

1991

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“…Plasmid analysis suggests much antibiotic resistance to be chromosomally determined as strains with the SSP alone possessed resistance markers and have been previously reported [22].…”

Section: Discussionmentioning

confidence: 87%

Diversity and molecular variation among plasmids inSalmonella entericaserotype Dublin based on restriction enzyme fragmentation pattern analysis

1995

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SUMMARYMolecular variation within and between plasmids ofSalmonella enterica serotype Dublin was analysed. Such variation has been demonstrated in the serotypespecific plasmids (SSP's) of Typhimurium and Enteritidis. The two aims of this study were to determine the plasmid diversity in a host-adapted serotype and also the incidence of molecular variation in the SSP among strains of Dublin using restriction endonuclease fragmentation pattern (REFP) analysis with Pstl, Smal and EcoRV. Sixty-five strains were examined from seven countries. Plasmid profile and REFP analysis showed that none of the strains was plasmid-free. Seventy-seven percent of the strains possessed the 72 kb SSP either alone or in combination with another plasmid; 23 % harboured plasmids which were molecular variants of the SSP. Four of the variants were more closely related to each other than to the reference SSP and were harboured by Dublin isolated from both the USA and Europe. A further three were shown to be cointegrate plasmids and were similarly distributed. Thirty-two percent of strains possessed the SSP alone. None of the UK strains was resistant to any of the antimicrobial agents tested whereas 74 % of the remaining strains were resistant to between one and five antimicrobial agents. This study corroborates previous findings concerning the high degree of stability of the SSP and confirmed the clonal nature of Dublin.Co-resident plasmids provided evidence of sub-clones within localized geographical areas.

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“…Similarly, the marked difference in the antimicrobial sensitivity of isolates of S Dublin and S Typhimurium from cattle indicate the problems of ascribing cause and effect. Both serotypes are frequently isolated from cattle and are likely to be exposed to the same antimicrobial selection pressures, and resistance plasmids can readily be introduced in vitro into S Dublin (Woodward and others 1989). It has also been shown that when plasmids are introduced into S Dublin, chromosomal integration occurs (Helmuth and Seiler 1986, Woodward and others 1989), whereas in S Typhimurium, most resistance genes are carried on plasmids (Threlfall and others 1995).…”

Section: Discussionmentioning

confidence: 99%

“…Both serotypes are frequently isolated from cattle and are likely to be exposed to the same antimicrobial selection pressures, and resistance plasmids can readily be introduced in vitro into S Dublin (Woodward and others 1989). It has also been shown that when plasmids are introduced into S Dublin, chromosomal integration occurs (Helmuth and Seiler 1986, Woodward and others 1989), whereas in S Typhimurium, most resistance genes are carried on plasmids (Threlfall and others 1995). However, Threlfall and others (1994) have demonstrated the chromosomal integration of resistance genes in S Typhimurium DT104 isolated during the recent epidemic during the 1990s.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial resistance in Salmonella isolated from animals and their environment in England and Wales from 1988 to 1999

et al. 2002

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Resistance to 16 antimicrobial agents was monitored in 109,125 Salmonella cultures isolated from animals, their environment and feedstuffs between 1988 and 1999. The sensitivity of the 6512 isolates of Salmonella enterica enterica serotype Dublin to all the antimicrobial agents tested varied from 98.2 per cent in 1997 to 99.7 per cent in 1990 and 1996. In contrast, among 28,053 isolates of Salmonella enterica enterica serotype Typhimurium, there was a marked decrease in their sensitivity to all the antimicrobial agents tested, from 57.4 per cent in 1992 to 7.6 per cent in 1995, owing to the widespread occurrence in farm animals of S Typhimurium isolates of the definitive type DT104, resistant to ampicillin, sulphonamides, streptomycin, chloramphenicol and tetracyclines, although the percentage of sensitive isolates increased to 18.4 per cent in 1999, when the incidence of DT104 had decreased. Some isolates of DT104 also showed an increase in resistance to potentiated sulphonamides (2.4 per cent in 1989 to 19.2 per cent in 1999) and nalidixic acid (0 per cent in 1992, 3.8 per cent in 1995 to a peak of 16.9 per cent in 1998). In 1996, 5.1 per cent of 1086 isolates of S Typhimurium from cattle and 35.9 per cent of 192 isolates of S Typhimurium from poultry showed resistance to nalidixic acid. Of the other 74,528 Salmonella isolates, the percentage of strains sensitive to all the antimicrobials tested decreased slightly from 88.2 per cent in 1988 to 70.6 per cent in 1996 and then increased slightly to 73.7 per cent in 1999. The commonest of these other Salmonella serotypes was Salmonella Enteritidis (20,982), which remained predominantly susceptible (ranging from 81.4 to 97.4 per cent) during the study period. Few isolates were resistant to commonly used veterinary antimicrobials, for example, furazolidone, the use of which was banned in 1990, and the aminoglycoside, apramycin.

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Genetic evidence for a chromosomally integrated multiresistance plasmid in Salmonella dublin

Cited by 9 publications

References 17 publications

Instability of multiple drug resistance plasmids inSalmonella typhimuriumisolated from poultry

Instability of multiple drug resistance plasmids inSalmonella typhimuriumisolated from poultry

Diversity and molecular variation among plasmids inSalmonella entericaserotype Dublin based on restriction enzyme fragmentation pattern analysis

Antimicrobial resistance in Salmonella isolated from animals and their environment in England and Wales from 1988 to 1999

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