Genetic Engineering of Sunflower (Helianthus Annuus L.)

Everett, Nicholas P.; Robinson, K. E. P.; Mascarenhas, Desmond

doi:10.1038/nbt1187-1201

Cited by 78 publications

(32 citation statements)

References 20 publications

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“…One possible explanation for a low number of transformants in an otherwise highly efficient regeneration system is that the inhibitory action of the antibiotic on the non-transformed cells in the tissue somehow interferes with the process of regeneration of the transformed cells. This has already been claimed in sunflower [26] and may be operative in apple. Ways of obviating this are to lower the contact time, the concentration or both.…”

Section: ) the Selection Of Transformed Cells That Lead To The Regenmentioning

confidence: 74%

See 1 more Smart Citation

Regeneration and Transformation of Apple (Malus pumila Mill.)

James

Dandekar

1991

Plant Tissue Culture Manual

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Section: ) the Selection Of Transformed Cells That Lead To The Regenmentioning

confidence: 74%

“…For this recourse to a quantitative measure of the efficiency of transformation such as the GUS assay [ 17,26] may permit a more rapid assessment of the role of the variables discussed above.…”

Section: ) Cloning and Analyses Of Putative Transformantsmentioning

confidence: 99%

Regeneration and Transformation of Apple (Malus pumila Mill.)

James

Dandekar

1991

Plant Tissue Culture Manual

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“…At such moments transformed cells have the opportunity to act independently from neighbouring cells, otherwise they have to divide in commitment with untransformed cells. In indirect embryogenesis very young embryos are subjected to secondary embryogenesis and this explains the successful application of indirect embryogenesis for plant transformation in Apium graveolens (Catlin et al, 1988), Beta vulgaris (D' Halluin et al, 1992), Citrus sinensis (Hikado et al, 1982), C. reticulata (Hidaka et al, 1990), Daucus carota (Scott & Draper, 1987), Helianthus annuus (Everett et al, 1987), Medicago sativa (Pezzotti et al, 1991), Oryza sativa (Christou et al, 1991) and Zea mays (Gordon-Kamm et al, 1990).…”

Section: Plant Transformationmentioning

confidence: 99%

Secondary somatic embryogenesis and applications in plant breeding

Raemakers¹,

Jacobsen²,

Visser³

1995

Euphytica

156

107

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Secondary somatic embryogenesis is the phenomenon whereby new somatic embryos are initiated from somatic embryos. Such cultures have been described in at least 80 Gymnosperm and Angiosperm species. In the initial step (primary somatic embryogenesis) such cultures have to be started from plant explants. In general, primary somatic embryogenesis from vegetative plant explants is, indirect and mostly driven by auxin (AUX) or auxin and cytokinin (AUX/CYT) supplemented media, whereas, from zygotic embryos it is direct and driven, to a larger extent, by CYT or growth regulator free media. Primary somatic embryogenesis from floral plant explants is between these two extremes. Indirect and direct somatic embryogenesis should be seen as two extremes of one continuum: in indirect somatic embryogenesis the embryos develop up to the (pre)-globular stage and in direct somatic embryogenesis to mature stages before they are subjected to secondary embryogenesis. In general, secondary embryogenesis requires no growth regulators in species with CYT driven primary embryogenesis. Whereas, continuous exposure to growth regulators is needed in species with CYT/AUX or AUX driven primary embryogenesis.In most species somatic embryos can be converted into shoots, although the frequencies are mostly low. In general, somatic embryos induced by growth regulator free or CYT supplemented media meet more difficulties in shoot development than embryos induced by AUX supplemented media. Applications of secondary somatic embryogenesis for plant breeding are discussed.

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“…We arrive at the same conclusion on plant cells with a system where different morphogenetic steps are required. Everett et al [ 11 ] reported the inhibitory role of kanamycin on morphogenesis of embryogenic kanamycin-resistant calli in sunflower; this result, together with ours, confwrns the necessity to study the toxicity of the selective agent used when a new transformation-regeneration system is being developped.…”

Section: Discussionmentioning

confidence: 82%

Phleomycin resistance as a dominant selectable marker for plant cell transformation

et al. 1989

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Tobacco cells are sensitive to bleomycin and phleomycin. The Tn5 and the Streptoalloteichus hindustanus (Sh) bleomycin resistance ('Ble') genes conferring resistance to these antibiotics have each been inserted into two plant expression vectors. They are flanked by the nopaline synthase (nos) or the cauliflower mosaic virus (CaMV) 35S promoters on one side, and by the nos polyadenylation signal on the other. These four chimaeric genes were introduced into the binary transformation vector pGA 492, which were thereafter mobilized into Agrobacterium tumefaciens strain LBA 4404. The resulting strains were used to transform Nicotiana tabacum cv. Xanthi nc using the leaf disc transformation procedure. In all cases, phleomycin- and bleomycin-resistant tobacco plants were regenerated from transformed cells under selective conditions; however, the highest frequency of rooted plants was obtained when transformation was carried out with the 'Sh Ble' gene under the control of the 35S promoter. Phleomycin resistance was stably transmitted to sexual offspring as a dominant nuclear trait as confirmed by Southern blotting.

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Genetic Engineering of Sunflower (Helianthus Annuus L.)

Cited by 78 publications

References 20 publications

Regeneration and Transformation of Apple (Malus pumila Mill.)

Regeneration and Transformation of Apple (Malus pumila Mill.)

Secondary somatic embryogenesis and applications in plant breeding

Phleomycin resistance as a dominant selectable marker for plant cell transformation

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