Regeneration and Transformation of Apple (Malus pumila Mill.)

James, D. J.; Dandekar, Abhaya M.

doi:10.1007/978-94-009-0103-2_19

Cited by 14 publications

(10 citation statements)

References 32 publications

(36 reference statements)

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“…in Vitis at 7 mg/l (Gray and Meredith 1992) and in Rubus at 10 mg/l (Fiola et al 1990). In Malus, regeneration was inhibited with 20 mg/l kanamycin for the cultivar Greensleeves (James and Dandekar 1991).…”

Section: Resultsmentioning

confidence: 99%

Genetic transformation of European chestnut

Seabra¹,

Pais

1998

Plant Cell Reports

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Stable incorporation of the nptII gene into Castanea sativa Mill. has been achieved by Agrobacteriummediated transformation. The transformation assays were performed by infecting wounded hypocotyls with a strain of Agrobacterium tumefaciens, LBA 4404 harbouring the plasmid p35SGUSINT. Although two schemes of selection were tested, many escapes were obtained. The best strategy to avoid this problem is the introduction of higher concentrations of kanamycin in the culture medium, immediately after coculture. PCR analysis showed of the selectable nptII gene integration in the plant genome. β-Glucuronidase histochemical assay revealed the expression of the uidA gene in shoots, regenerated from transformed explants.

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Section: Resultsmentioning

confidence: 99%

Genetic transformation of European chestnut

Seabra¹,

Pais

1998

Plant Cell Reports

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“…Adventitious roots never appeared at any part of the uninfected androgenic embryos. As non-transformed horse chestnut roots did not grow in PGR-free medium, we avoided using antibiotics or herbicides as selective agents, which is a major advantage as these generally allow the forming of chimera and have negative impact on proliferation and differentiation (James and Dandekar 1991). Most explant cells die during negative selection, which may cause a release of toxic substances (such as phenolics) that may affect transformed cells (Bowen 1992).…”

Section: Discussionmentioning

confidence: 99%

Agrobacterium rhizogenes -mediated DNA transfer to Aesculu s hippocastanum L. and the regeneration of transformed plants

et al. 2004

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Hairy roots were induced from androgenic embryos of horse chestnut (Aesculus hippocastanum L.) by infection with Agrobacterium rhizogenes strain A4GUS. Single roots were selected according to their morphology in the absence of antibiotic or herbicide resistance markers. Seventy-one putative transformed hairy root lines from independent transformation events were established. Regeneration was induced in MS liquid medium supplemented with 30 microM 6-benzylaminopurine (BA), and the regenerants were multiplied on MS solid medium containing 10 microM BA. Following elongation on MS medium supplemented with 1 microM BA and 500 mg/l polyvinylpyrrolidone, the shoots were subjected to a root-inducing treatment. Stable integration of TL-DNA within the horse chestnut genome was confirmed by Southern hybridization. The copy number of transgenes was estimated to be from two to four.

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“…Although shoot number was not affected by leaf excision time, shoots were larger when produced on explants excised 15 days after the beginning of subculture compared to those produced on leaves excised on the 30th day (Famiani et al 1994). Leaves collected from rooted shoots were found to be more suitable for regeneration (James et al 1988;James and Dandekar 1991). This might be because of inter-shoot competition for light and nutrients of micropropagated cultures.…”

Section: Shoot Regeneration In Vitro: Organogenesismentioning

confidence: 91%

The role of cytokinins in shoot organogenesis in apple

Magyar-Tábori

Dobránszki

Silva

et al. 2010

Plant Cell Tiss Organ Cult

129

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Effective regeneration in vitro is a necessary precondition for the implementation of different biotechnological approaches in plant breeding. Numerous studies have reported on regeneration from apple somatic tissues, and organogenesis has been proved to be influenced by several factors including mother shoots (genotype, size, type, and age of explant), in vitro conditions (dark period, light intensity, and quality), and others (wounding, orientation of leaf explants). However, one of the most important factors before and during the regeneration process is the type and concentration of cytokinin applied. Thidiazuron and benzyladenine are the most frequently used cytokinins in the regeneration systems, but their efficiency depends on genotype and other factors. Other cytokinins (e.g., zeatin and kinetin) have also been tested in several experiments and they were found in general to be less active. The organogenic ability of explants can also be increased by a properly selected cytokinin pre-treatment. Cytokinins applied in the pre-treatments can influence the leaf structure, which in turn can alter the regeneration capacity of the leaf explant. Interactions between factors of pre-treatments (hormones, light, and culture conditions) and factors of the regeneration phase should be considered. This review brings into focus the role of different cytokinins during in vitro shoot development, discussing their effects on the histology of leaves developed in vitro, and how this affects the subsequent regeneration process.

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Regeneration and Transformation of Apple (Malus pumila Mill.)

Cited by 14 publications

References 32 publications

Genetic transformation of European chestnut

Genetic transformation of European chestnut

Agrobacterium rhizogenes -mediated DNA transfer to Aesculu s hippocastanum L. and the regeneration of transformed plants

The role of cytokinins in shoot organogenesis in apple

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