Genetic Engineering of Miscanthus

Engler, Dean E.; Jakob, Katrin

doi:10.1007/978-1-4419-5947-8_12

Cited by 6 publications

(8 citation statements)

References 313 publications

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“…For the selection of transformed calli and transgenic plants, we initially used hygromycin antibiotics for the selection of transgenic plants following the transformation with pCAMBIA1301 vector. Miscanthus species was known to be susceptible to many of antibiotics and herbicides including hygromycin and BASTA, but not kanamycin (Engler and Jakob 2013). The results of molecular analyses showed that all the putative transgenic plants obtained from the hygromycin resistance selection were transgenic plants with transgenes (Fig.…”

Section: Discussionmentioning

confidence: 97%

“…The important steps for Agrobacterium-mediated transformation of crops include the induction of regenerable embryogenic calli, DNA delivery into the embryogenic calli by the inoculation and co-cultivation of Agrobacterium cells harboring a vector with target gene(s), and the selection of transformed calli and regeneration of transgenic plants (Kim et al 2007;Engler and Jakob 2013). Thus, the first critical step for the success of the genetic transformation might be the step to obtain embryogenic calli which has high regeneration potentials.…”

Section: Discussionmentioning

confidence: 99%

“…However, this type of production appears unsustainable because of the potential competition with food and feed production. Therefore, there is extensive interest in lignocellulosic ethanol, because lignocellulosic biomass is an abundant, domestic, renewable source that can be converted to liquid transportation fuels (Yuan et al 2008;Carroll and Somerville 2009;Balat 2011;Engler and Jakob 2013). Lignocellulosic feedstock for biofuel production is mostly C4 grasses that are characterized by high productivity and resource use efficiency, which includes Miscanthus species (van der Weijde et al 2013).…”

Section: Introductionmentioning

confidence: 99%

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Agrobacterium-mediated genetic transformation of Miscanthus sinensis

Hwang

Cho

Han

et al. 2013

Plant Cell Tiss Organ Cult

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Miscanthus species are tall perennial rhizomatous grasses with C4 photosynthesis originating from East Asia, and they are considered as important bioenergy crops for biomass production. In this study, Agrobacterium-mediated transformation system for M. sinensis was developed using embryogenic calli derived from mature seeds. In order to establish a stable system, optimum conditions to obtain highly regenerable and transformation-competent embryogenic calli were investigated, and embryogenic calli were efficiently induced with callus induction medium containing 3 mg L -1 2,4-dichlorophenoxyacetic acid and 25 mM L-proline, at pH 5.7 with an induction temperature of 28°C. In addition, the embryogenic callus induction and regeneration potentials were compared between seven M. sinensis germplasms collected from several sites in Korea, which revealed that the germplasm SNU-M-045 had superior embryogenic callus induction and regeneration potentials. With this germplasm, the genetic transformation of M. sinensis was performed using Agrobacterium tumefaciens EHA105 carrying pCAM-BIA1300 with a green fluorescence protein gene as a reporter. After putative transgenic plants were obtained, the genomic integration of transgenes was confirmed by genomic PCR, transgene expression was validated by Northern blot analysis, and the number of transgene integration was confirmed by DNA gel blot analysis. Furthermore, the Agrobacteriummediated transformation of M. sinensis was also performed with pCAMBIA3301 which contains an herbicide resistance gene (BAR), and we obtained transgenic M. sinensis plants whose herbicide resistance was confirmed by spraying with BASTA Ò . Therefore, we have established a stable Agrobacterium-mediated transformation system for M. sinensis, and also successfully produced herbicide-resistant Miscanthus plants by introducing BAR gene via the established method.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Agrobacterium-mediated genetic transformation of Miscanthus sinensis

Hwang

Cho

Han

et al. 2013

Plant Cell Tiss Organ Cult

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“…Thus for most of these grasses, the exception being maize, major progress is required in the development and optimization of transformation protocols. Reviews on the status of transformation of sorghum (Howe et al, 2006; Girijashankar and Swathisree, 2009), switchgrass (Somleva et al, 2002; Conger, 2003; Bouton, 2007; Burris et al, 2009; Xi et al, 2009; Saathoff et al, 2011), miscanthus (Wang et al, 2011; Engler and Jakob, 2013) and sugarcane (Santosa et al, 2004; Hotta et al, 2010) provide further information. However, transgenic approaches are regarded with great caution in dedicated bioenergy crops as well, as they are mostly outcrossing perennial grasses (Wang and Brummer, 2012).…”

Section: Genetic Improvementmentioning

confidence: 99%

The potential of C4 grasses for cellulosic biofuel production

Weijde¹,

Kamei²,

Torres³

et al. 2013

Front. Plant Sci.

166

138

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† These authors have contributed equally to this work.With the advent of biorefinery technologies enabling plant biomass to be processed into biofuel, many researchers set out to study and improve candidate biomass crops. Many of these candidates are C4 grasses, characterized by a high productivity and resource use efficiency. In this review the potential of five C4 grasses as lignocellulosic feedstock for biofuel production is discussed. These include three important field crops-maize, sugarcane and sorghum-and two undomesticated perennial energy grasses-miscanthus and switchgrass. Although all these grasses are high yielding, they produce different products. While miscanthus and switchgrass are exploited exclusively for lignocellulosic biomass, maize, sorghum, and sugarcane are dual-purpose crops. It is unlikely that all the prerequisites for the sustainable and economic production of biomass for a global cellulosic biofuel industry will be fulfilled by a single crop. High and stable yields of lignocellulose are required in diverse environments worldwide, to sustain a year-round production of biofuel. A high resource use efficiency is indispensable to allow cultivation with minimal inputs of nutrients and water and the exploitation of marginal soils for biomass production. Finally, the lignocellulose composition of the feedstock should be optimized to allow its efficient conversion into biofuel and other by-products. Breeding for these objectives should encompass diverse crops, to meet the demands of local biorefineries and provide adaptability to different environments. Collectively, these C4 grasses are likely to play a central role in the supply of lignocellulose for the cellulosic ethanol industry. Moreover, as these species are evolutionary closely related, advances in each of these crops will expedite improvements in the other crops. This review aims to provide an overview of their potential, prospects and research needs as lignocellulose feedstocks for the commercial production of biofuel.

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“…Although genetic transformation of Miscanthus using particle bombardment (Wang et al, 2011) and Agrobacterium -mediated transformation (Engler and Jakob, 2013) has been reported there is, to our knowledge, no report on the functional analysis of transgenes expressed in Miscanthus . An efficient Agrobacterium -mediated transformation protocol, preferably for high yielding Miscanthus genotypes, needs to be developed to enable the effective improvement of key traits through genetic engineering approaches.…”

Section: Molecular Biology Of Cell Wall Biosynthesismentioning

confidence: 99%

Advances in the genetic dissection of plant cell walls: tools and resources available in Miscanthus

2013

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Tropical C4 grasses from the genus Miscanthus are believed to have great potential as biomass crops. However, Miscanthus species are essentially undomesticated, and genetic, molecular and bioinformatics tools are in very early stages of development. Furthermore, similar to other crops targeted as lignocellulosic feedstocks, the efficient utilization of biomass is hampered by our limited knowledge of the structural organization of the plant cell wall and the underlying genetic components that control this organization. The Institute of Biological, Environmental and Rural Sciences (IBERS) has assembled an extensive collection of germplasm for several species of Miscanthus. In addition, an integrated, multidisciplinary research programme at IBERS aims to inform accelerated breeding for biomass productivity and composition, while also generating fundamental knowledge. Here we review recent advances with respect to the genetic characterization of the cell wall in Miscanthus. First, we present a summary of recent and on-going biochemical studies, including prospects and limitations for the development of powerful phenotyping approaches. Second, we review current knowledge about genetic variation for cell wall characteristics of Miscanthus and illustrate how phenotypic data, combined with high-density arrays of single-nucleotide polymorphisms, are being used in genome-wide association studies to generate testable hypotheses and guide biological discovery. Finally, we provide an overview of the current knowledge about the molecular biology of cell wall biosynthesis in Miscanthus and closely related grasses, discuss the key conceptual and technological bottlenecks, and outline the short-term prospects for progress in this field.

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Genetic Engineering of Miscanthus

Cited by 6 publications

References 313 publications

Agrobacterium-mediated genetic transformation of Miscanthus sinensis

Agrobacterium-mediated genetic transformation of Miscanthus sinensis

The potential of C4 grasses for cellulosic biofuel production

Advances in the genetic dissection of plant cell walls: tools and resources available in Miscanthus

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