Genetic engineering of indica rice with AtDREB1A gene for enhanced abiotic stress tolerance

Latha, G Manju; Raman, K. Venkat; Lima, John Milton; Pattanayak, Debasis; Singh, Ashok Kumar; Chinnusamy, Viswanathan; Bansal, Kailash C.; Rao, K. R. S. Sambasiva; Mohapatra, Trilochan

doi:10.1007/s11240-018-1505-7

Cited by 13 publications

(9 citation statements)

References 42 publications

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“…Furthermore, after water recovery time the non-transgenic plants showed a higher percentage of chlorotic and necrotic leaves (Figure 2b-d), indicating that DREB1a confers tolerance to more severe drought in rice. These observations are consistent with previous studies that showed drought tolerance in vegetative stages for indica (Datta et al 2012;Latha et al 2019) and japonica (Oh et al 2005) rice cultivars.…”

Section: Discussionsupporting

confidence: 93%

“…More recently, Latha et al (2019) performed microarray on flag leaf of transgenic (RD29a:DREB1a) indica rice subjected to drought at the booting stage for 14 days. They found upregulation of a large set of genes associated with chloroplast structure and function and suggested enhanced photosynthesis during stress as part of the mechanism for transgenic rice to withstand stress.…”

Section: Discussionmentioning

confidence: 99%

“…They also identified a few upregulated genes in the DREB1a transgenic rice. Finally, Latha et al (2019) using microarray showed induction of DRE-containing genes encoding chloroplast structure and function in RD29a:DREB1a indica rice exposed to drought at reproductive stage. Similarly, RNA-seq analysis of Salvia sp.…”

Section: Introductionmentioning

confidence: 95%

“…Further, since RD29a is a stress-induced gene, it could promote DREB1a expression sustainably during stress conditions. RD29a promoter induced DREB1a transcripts to 2 -10-fold higher under salinity, cold, and drought conditions in Arabidopsis, rice, and peanut (Latha et al, 2019;Oh et al, 2005;Sarkar et al, 2016), and transgenic expression of RD29a:DREB1a conferred tolerance to salinity, drought, and cold stress in diverse plant species including rice (Datta et al, 2012;Ganguly et al, 2020;Geda et al, 2019;Latha et al, 2019;Muthurajan et al, 2021;Oh et al, 2005;Ravikumar et al, 2014;), peanuts (Bhalani et al, 2019;Bhatnagar-Mathur et al, 2007;Devi et al, 2011;Sarkar et al, 2016) chickpea (Anbazhagan et al, 2015), soybean (de Paiva et al, 2014), chrysanthemum (Hong et al, 2006), potato (Behnam et al, 2007;Shimazaki et al, 2016) and Salvia sp. (Wei et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Phenotypic and transcriptomic analysis reveals early stress responses in transgenic rice expressing Arabidopsis DREB1a

Berchembrock

Pathak

Maurya

et al. 2022

Preprint

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Overexpression of Arabidopsis Dehydration Response Element Binding 1a (DREB1a) is a well-known approach for developing salinity, cold and/or drought stress tolerance. However, understanding of the genetic mechanisms associated with DREB1a expression in rice is generally limited. In this study, DREB1a associated early responses were investigated in a transgenic rice line harboring cold-inducible DREB1a at a gene stacked locus. While the function of other genes in the stacked locus was not relevant to stress tolerance, this study demonstrates DREB1a can be colocalized with other genes for multigenic trait enhancement. As expected, the transgenic lines displayed improved tolerance to salinity stress and water withholding when compared to non-transgenic controls. RNA sequencing and transcriptome analysis showed upregulation of complex transcriptional networks and metabolic reprogramming as DREB1a expression led to the upregulation of multiple transcription factor gene families, suppression of photosynthesis and induction of secondary metabolism. In addition to the detection of previously described mechanisms such as production of protective molecules, potentially novel pathways were also revealed. These include jasmonate, auxin, and ethylene signaling, induction of JAZ and WRKY regulons, trehalose synthesis and polyamine catabolism. These genes regulate various stress responses and ensure timely attenuation of the stress signal. Furthermore, genes associated with heat stress response were downregulated in DREB1a overexpressing lines, suggesting antagonism between heat and dehydration stress pathways. In summary, through a complex transcriptional network, multiple stress signaling pathways are induced by DREB1a that presumably lead to early perception and rapid response towards stress tolerance as well as attenuation of the signal to prevent deleterious effects of the runoff response.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Phenotypic and transcriptomic analysis reveals early stress responses in transgenic rice expressing Arabidopsis DREB1a

Berchembrock

Pathak

Maurya

et al. 2022

Preprint

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“…Rice is among the signi cantly important cereal crops in the world. Stable gene expression in rice tissues is an important event during transformation [43,44]. The recovery of transgenic plants in the presence of bialaphos has been greatly facilitated by the bar gene [45].…”

Section: Discussionmentioning

confidence: 99%

Genetic Transformation of two Turkish rice (Oryza sativa L.) cutivars through Agrobacterium tumefaciens

Kahriz

Khawar

2022

Preprint

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Background Seed weevils are one of the causes of post harvest rice yield losses in Turkey. There are many cultural and chemical methods to control these insects.PurposeThis study aimed to control these using Agrobacterium tumefaciens mediated genetic transformation in two rice cultivars namely Hamzadere and Osmancı-97.Methods and results Two days old germinated seeds of the two cultivars with 2-3 mm long shoots were used as explants. They were treated with Agrobacterium tumefciens containing a pTF101AoPR1AcBar plant expression vector containing the cry1Ac gene under the control of the wound-induced (AoPR1) promoter. The transformation event was genotype-dependent. Out of 3000 and 2600 explants of cv Hamzadere and cv Osmancık-97, only 92 (3.07%) and 32 (1.230%) plants were selected on MS medium containing 3.0 and 2.5 mg/l bialaphos. These were validated using PCR, bioassay, and ELISA tests. All plants were held in the greenhouse until T0 and T1 seed settings. three (0.10%) plants of cv Hamzadere and 5 (0.19%) plants of cv. Osmancık 97 could be validated through all tests in T₀ and T₁ generations in both cultivars of rice for the first time using a novel and a very simple transformation procedure.ConclusionThese results are very encouraging and will influence future rice transformation techniques and improve rice breeding technologies.

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Phenotypic and transcriptomic analysis reveals early stress responses in transgenic rice expressing Arabidopsis DREB1a

et al. 2022

View full text Add to dashboard Cite

Overexpression of Arabidopsis dehydration response element binding 1a ( DREB1a ) is a well‐known approach for developing salinity, cold and/or drought stress tolerance. However, understanding of the genetic mechanisms associated with DREB1a expression in rice is generally limited. In this study, DREB1a ‐associated early responses were investigated in a transgenic rice line harboring cold‐inducible DREB1a at a gene stacked locus. Although the function of other genes in the stacked locus was not relevant to stress tolerance, this study demonstrates DREB1a can be co‐localized with other genes for multigenic trait enhancement. As expected, the transgenic lines displayed improved tolerance to salinity stress and water withholding as compared with non‐transgenic controls. RNA sequencing and transcriptome analysis showed upregulation of complex transcriptional networks and metabolic reprogramming as DREB1a expression led to the upregulation of multiple transcription factor gene families, suppression of photosynthesis, and induction of secondary metabolism. In addition to the detection of previously described mechanisms such as production of protective molecules, potentially novel pathways were also revealed. These include jasmonate, auxin, and ethylene signaling, induction of JAZ and WRKY regulons, trehalose synthesis, and polyamine catabolism. These genes regulate various stress responses and ensure timely attenuation of the stress signal. Furthermore, genes associated with heat stress response were downregulated in DREB1a expressing lines, suggesting antagonism between heat and dehydration stress response pathways. In summary, through a complex transcriptional network, multiple stress signaling pathways are induced by DREB1a that presumably lead to early perception and prompt response toward stress tolerance as well as attenuation of the stress signal to prevent deleterious effects of the runoff response.

show abstract

Genetic engineering of indica rice with AtDREB1A gene for enhanced abiotic stress tolerance

Cited by 13 publications

References 42 publications

Phenotypic and transcriptomic analysis reveals early stress responses in transgenic rice expressing Arabidopsis DREB1a

Phenotypic and transcriptomic analysis reveals early stress responses in transgenic rice expressing Arabidopsis DREB1a

Genetic Transformation of two Turkish rice (Oryza sativa L.) cutivars through Agrobacterium tumefaciens

Phenotypic and transcriptomic analysis reveals early stress responses in transgenic rice expressing Arabidopsis DREB1a

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