Genetic Engineered Ideonella Sakaiensis Bacteria: A Solution of the Legendary Plastic Waste Problem

Widyastuti, Galuh

doi:10.2139/ssrn.3194556

Cited by 3 publications

(4 citation statements)

References 4 publications

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“…The bacteria are positive for catalase and cytochrome oxidation tests. It tests positive for uptake of NAG, maltose, adipic acid and potassium gluconate and negative for denitrification, NO 3reduction, indole formation and sugar (glucose) fermentation in the API 20 NE test, â-galactosidase and D-glucose assimilation (Table 1) 14,15,11 .…”

Section: Discovery and Properties Of Ideonella Sakaiensismentioning

confidence: 99%

“…The bacterium has a DNA G+C content of about 70.4mol%. Phylogenetic, biological, biochemical, and physiological studies reveal the bacteria as a new representative of the genus Ideonella, named I. sakaiensis 14,15,11 . Fig.…”

Section: Discovery and Properties Of Ideonella Sakaiensismentioning

confidence: 99%

“…These compounds, once formed, can be further biodegraded for the cell's metabolic needs, purified and then can be recycled. The monomers formed are safe for the environment, unlike PET which can be highly inert and pose a serious threat to the environment if Figure 2 not recycled 15,18 .…”

Section: Pet Degrading Ability Of Ideonella Sakaiensismentioning

confidence: 99%

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Recent Advancements and Mechanism of Plastics Biodegradation Promoted by Bacteria: A Key for Sustainable Remediation for Plastic Wastes

Kaur

Sharma

Shree

et al. 2023

Biosci., Biotech. Res. Asia

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Plastic has become an indispensable part of our lives and cutting down plastic consumption entirely is difficult to achieve. The recalcitrant and non-biodegradable nature of plastic leads to accumulation of tons of plastic in landfills and water bodies which further risks marine life and human life too causing serious health issues. In recent years, several microbial enzymes have been discovered that have the ability to degrade plastic. The present review highlights the recent discovery and properties of the plastic-eating bacteria, Ideonella sakaiensis, that has potential to be used for plastic degradation and recycling. The bacteria possess unique enzymes that allow it to utilise Polyethylene terephthalate (PET) plastic, thereby degrading it to relatively safer monomeric forms that can be further degraded and purified to manufacture recycled plastics. The review focuses on the mechanism of PET hydrolysis, recent advances in the field to escalate enzymatic efficiency and development of new bacterial and enzymatic strains through genetic engineering which can enhance its catalytic competence and make the process time and cost-effective. The plastic metabolising bacteria can thus be a potential and efficient bio-alternative to degrade plastic in a biological and sustainable manner thereby helping scale the otherwise insurmountable plastic pollution crisis.

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Section: Discovery and Properties Of Ideonella Sakaiensismentioning

confidence: 99%

Section: Discovery and Properties Of Ideonella Sakaiensismentioning

confidence: 99%

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Recent Advancements and Mechanism of Plastics Biodegradation Promoted by Bacteria: A Key for Sustainable Remediation for Plastic Wastes

Kaur

Sharma

Shree

et al. 2023

Biosci., Biotech. Res. Asia

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show abstract

“…To help dampen this astounding issue, Moog et al, utilized Phaeodactylum tricornutum microalga to produce and secrete the PETase enzyme for the degradation of both PET and polyethylene terephthalate glycol (PETG) in saltwater analysis showed that such PETase possessed degradation activity against both PET and PETG, including under moderate conditions and in saltwater [ 43 ] ( Figure 2 a,b respectively). While Ideonella sakaiensis bacteria was considered for its ability to produce the PETase, this specie showed limited performance in ocean-like environments presumably due to its instability and inability to survive in such aqueous habitats [ 43 , 55 ].…”

Section: Brief Description Of Applications Of Enzymes In Consumer Industriesmentioning

confidence: 99%

Hyaluronic Acid Allows Enzyme Immobilization for Applications in Biomedicine

et al. 2022

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Enzymes are proteins that control the efficiency and effectiveness of biological reactions and systems, as well as of engineered biomimetic processes. This review highlights current applications of a diverse range of enzymes for biofuel production, plastics, and chemical waste management, as well as for detergent, textile, and food production and preservation industries respectively. Challenges regarding the transposition of enzymes from their natural purpose and environment into synthetic practice are discussed. For example, temperature and pH-induced enzyme fragilities, short shelf life, low-cost efficiency, poor user-controllability, and subsequently insufficient catalytic activity were shown to decrease pertinence and profitability in large-scale production considerations. Enzyme immobilization was shown to improve and expand upon enzyme usage within a profit and impact-oriented commercial world and through enzyme-material and interfaces integration. With particular focus on the growing biomedical market, examples of enzyme immobilization within or onto hyaluronic acid (HA)-based complexes are discussed as a definable way to improve upon and/or make possible the next generation of medical undertakings. As a polysaccharide formed in every living organism, HA has proven beneficial in biomedicine for its high biocompatibility and controllable biodegradability, viscoelasticity, and hydrophilicity. Complexes developed with this molecule have been utilized to selectively deliver drugs to a desired location and at a desired rate, improve the efficiency of tissue regeneration, and serve as a viable platform for biologically accepted sensors. In similar realms of enzyme immobilization, HA’s ease in crosslinking allows the molecule to user-controllably enhance the design of a given platform in terms of both chemical and physical characteristics to thus best support successful and sustained enzyme usage. Such examples do not only demonstrate the potential of enzyme-based applications but further, emphasize future market trends and accountability.

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Biodegradation of different PET variants from food containers by Ideonella sakaiensis

et al. 2022

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The accumulation of macro-, micro- and nano-plastic wastes in the environment is a major global concern, as these materials are resilient to degradation processes. However, microorganisms have evolved their own biological means to metabolize these petroleum-derived polymers, e.g., Ideonella sakaiensis has recently been found to be capable of utilizing polyethylene terephthalate (PET) as its sole carbon source. This study aims to prove its potential capacity to biodegrade two commercial PET materials, obtained from food packaging containers. Plastic pieces of different crystallinity were simultaneously introduced to Ideonella sakaiensis during a seven-week lasting investigation. Loss in weight, appearance of plastics, as well as growth of Ideonella sakaiensis—through quantitative real-time PCR—were determined. Both plastics were found enzymatically attacked in a two-stage degradation process, reaching biodegradation capacities of up to 96%. Interestingly, the transparent, high crystallinity PET was almost fully degraded first, followed by the colored low-crystallinity PET. Results of quantitative real-time PCR-based gene copy numbers were found in line with experimental results, thus underlining its potential of this method to be applied in future studies with Ideonella sakaiensis.

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Genetic Engineered Ideonella Sakaiensis Bacteria: A Solution of the Legendary Plastic Waste Problem

Cited by 3 publications

References 4 publications

Recent Advancements and Mechanism of Plastics Biodegradation Promoted by Bacteria: A Key for Sustainable Remediation for Plastic Wastes

Recent Advancements and Mechanism of Plastics Biodegradation Promoted by Bacteria: A Key for Sustainable Remediation for Plastic Wastes

Hyaluronic Acid Allows Enzyme Immobilization for Applications in Biomedicine

Biodegradation of different PET variants from food containers by Ideonella sakaiensis

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