Genetic Effects of Scots Pine (Pinus Sylvestris L.) Domestication

Szmidt, Alfred E.; Muona, Outi

doi:10.1007/978-3-642-48125-3_16

Cited by 34 publications

(21 citation statements)

References 8 publications

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“…This result can be explained by more random and efficient genome sampling by RAPDs than by allozymes which are restricted to genes coding for proteins. The RAPD distance and GST coefficients for the investigated populations were low and comparable to similar measures for P sylvestris populations from Scandinavia based on allozymes (Szmidt & Muona, 1985;Szmidt & Wang, 1993; this study). Low levels of population differentiation for both types of markers found in this study can be explained by unrestricted gene flow among populations and the relatively recent history of P sylvestris in Scandinavia.…”

Section: Discussionsupporting

confidence: 82%

“…The material analysed in this study came from two mature stands of P sylvestris. Selection against the more homozygous, relatively inbred individuals was the likely cause of the negative fixation indices observed in this and other studies (e.g Szmidt & Muona, 1985;Plessas & Strauss, 1986;Kärkkäinen & Savolainen, 1993). It thus appears that when complete genotype information is available, analysis of RAPD variation yields population genetic parameters that are comparable to those based on allozymes.…”

Section: Discussionsupporting

confidence: 66%

“…When data for codominant markers are available, the estimated RAPD allele frequency can be corrected for potential deviations from HWE (Chong et a!., 1994). Studies employing allozymes suggest that wind-pollinated conifers, including Pinus sylvestris (L.), generally show only modest departures from HWE for this type of marker (Szmidt & Muona, 1985;Plessas & Strauss, 1986). This suggests that the same may be true for RAPD markers (Wu et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

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Empirical assessment of allozyme and RAPD variation in Pinus sylvestris (L.) using haploid tissue analysis

Szmidt

Wang

1996

Heredity

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We analysed 20 allozyme and 22 putative random amplified polymorphic DNA (RAPD) loci in two populations of Pinus sylvestris (L.) from northern Sweden. Genotypes for individual allozyme and RAPD loci were inferred from segregation patterns in haploid macrogametophytes. Therefore, it was possible to distinguish between homo-and heterozygotes carrying a RAPD fragment and to estimate directly the frequencies of RAPD fragments. The percentage of polymorphic loci and the expected and observed heterozygosity were lower for allozymes than for RAPDs. Average fixation indices for both types of markers were negative indicating a heterozygote excess over panmictic expectations. The apportionment of genetic variation within and among the investigated populations was similar for allozymes and RAPDs and showed that most of the variation resided within populations. RAPD genotypes inferred from haploid material were subsequently converted to diploid phenotypes and used to estimate indirectly the frequencies of RAPD fragments. Gene diversity measurements derived from indirectly estimated RAPD frequencies were distinctly lower than those based on directly estimated RAPD frequencies. This result was caused by the absence of the null homozygote at many loci which appeared as monomorphic in the diploid data set. Population differentiation coefficients based on the indirectly estimated RAPD frequencies were not concordant with those based on directly estimated RAPD frequencies. Our present results indicate that when complete genotype information can be obtained, RAPD analysis provides genetic information similar to that revealed by analysis of allozyme variation. On the other hand, our results are concordant with theoretical results suggesting that analysis of RAPD variation in diploid material can produce unreliable estimates of population-genetic parameters.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionsupporting

confidence: 66%

Section: Introductionmentioning

confidence: 99%

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Empirical assessment of allozyme and RAPD variation in Pinus sylvestris (L.) using haploid tissue analysis

Szmidt

Wang

1996

Heredity

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“…Szmidt 1984, Guldberg et al 198, Muona and Harju 1989, Wang et al 1991, Goncharenko et al 1994, Lewandowski et al 2000. Except one, all analyzed populations were in Hardy-Weinberg equilibrium (statistically significant excess of homozygotes was detected in planted progeny (K2) from Krotoszyn).…”

Section: Discussionmentioning

confidence: 96%

Genetic variability of Scots pine maternal populations and their progenies

Kosińska¹,

Lewandowski²,

Chalupka³

2007

Silva Fenn.

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The genetic variability of Scots pine was investigated in six populations from Poland representing two maternal populations and their natural and artificial progenies. Thirteen enzyme systems controlled by 2 allozyme loci were analyzed using starch gel electrophoresis. Progeny populations maintained a high and similar level of genetic variation to that observed in the maternal populations. As expected, much closer genetic relationships were observed between maternal populations and their respective progeny than between the two maternal populations themselves. Progeny populations had the same major alleles, but differed in the number of rare alleles. Therefore, probably not all rare alleles were transferred from the maternal stands to the progenies. In addition, new rare alleles appeared in the progeny populations, possibly as a result of external pollen flow into the maternal populations.

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“…Finnish Scots pine populations have high levels of genetic diversity both in natural stands and seed orchards, the average expected heterozygosities at enzyme loci ranging from 0.243 to 0.286 (Muona & Harju, 1989). No latitudinal differentiation in isozyme allele frequencies has been observed in Northern Europe, as the GST values are less than 0.02 (Gullberg et a!., 1985;Szmidt & Muona, 1985). In contrast, quantitative genetic traits related to climatic constraints, e.g.…”

Section: Discussion Rdna Variability Within Scots Pine Individualsmentioning

confidence: 99%

Variation and inheritance of ribosomal DNA in Pinus sylvestris L. (Scots pine)

Karvonen

Savolainen

1993

Heredity

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Variability in the ribosomal DNA (rDNA) gene family was surveyed in Scots pine (Pinus sylvestris L.) populations from southern and northern Finland. A total of 97 trees were studied with three restriction enzymes, revealing 13 variable rDNA phenotypes. Each rDNA phenotype represents the combined genotype of the eight rDNA loci (NORs, nucleolus organizer regions) that are present in the Scots pine genome. The studied Scots pine populations were equally variable and there was no evidence of geographic differentiation. Of the total rDNA diversity of the species, 86 per cent was found within populations (including within-individual variability) and 14 per cent was found between populations. Within individual trees, one to four rDNA repeat types were distinguished. No variation was found in the coding regions but variable restriction sites were identified in the IGS and transcribed spacer regions. The inheritance pattern of an rDNA variant carrying a 0.4 kb deletion in the transcribed spacer region was studied. The deletion-carrying rDNA variants were distributed non-randomly across the NOR loci and showed regular Mendelian segregation in the progeny. The observed distribution pattern of rDNA variability suggests that the rate of intrachromosomal homogenization is greater than that of interchromosomal homogenization in Scots pine.

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Genetic Effects of Scots Pine (Pinus Sylvestris L.) Domestication

Cited by 34 publications

References 8 publications

Empirical assessment of allozyme and RAPD variation in Pinus sylvestris (L.) using haploid tissue analysis

Empirical assessment of allozyme and RAPD variation in Pinus sylvestris (L.) using haploid tissue analysis

Genetic variability of Scots pine maternal populations and their progenies

Variation and inheritance of ribosomal DNA in Pinus sylvestris L. (Scots pine)

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