Variation and inheritance of ribosomal DNA in Pinus sylvestris L. (Scots pine)

Karvonen, P.; Savolainen, Outi

doi:10.1038/hdy.1993.186

Cited by 63 publications

(31 citation statements)

References 38 publications

(35 reference statements)

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“…PCR-RFLP is more advantageous than Southern blot since it is less time consuming, and also provides the analysis of a higher number of samples simultaneously (Halward et al 1992;Karvonen and Savolainen 1993).…”

Section: Introductionmentioning

confidence: 99%

Intergenic spacer length variants in Old Portuguese bread wheat cultivars

Carvalho

Guedes-Pinto

Lima-Brito

2011

J Genet

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The intergenic spacer of the ribosomal DNA is highly variable, but is location specific in the nucleolar organizer region of the chromosomes. This study provides an event of high level of polymorphism / size variation and occurrence of 14 unique phenotypes in 48 landraces of Portuguese bread wheat cultivars for IGS-amplified products obtained by PCR-RFLP technique performed with TaqI. The attendant IGS polymorphism has been used to deduce affinities between landraces. Some of the high molecular weight IGS allelic variants were also probed for their chromosomal localization by sequential silver nitrate staining and fluorescence in situ hybridization. However, only the intergenic spacer allelic variant of 3.1 kb could be successfully hybridized, and was observed to be physically located on the chromosome pair 1B in the NOR loci of the cultivar 'Magueija'.

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Section: Introductionmentioning

confidence: 99%

Intergenic spacer length variants in Old Portuguese bread wheat cultivars

Carvalho

Guedes-Pinto

Lima-Brito

2011

J Genet

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“…Ribosomal DNA differentiation was several times higher but still lower than among-population differentiation in bud-set of the same populations. No geographic trends could be revealed for the ribosomal DNA variation (Karvonen and Savolainen 1993). Szmidt et al (1996) reported no RAPD variation between two Swedish Pinus sylvestris populations from latitudes 64° and 67°, respectively.…”

Section: Isozymesmentioning

confidence: 99%

Evolutionary forces influencing variation among populations of Pinus sylvestris

Eriksson¹

1998

Silva Fenn.

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Eriksson, G. 1998. Evolutionary forces influencing variation among populations of Pinus sylvestris. Silva Fennica 32(2): 173-184.The evolutionary forces influencing genetic differentiation among populations are identified. Natural selection, random genetic drift, and mutations promote differentiation while phenotypic plasticity and gene flow delay or prevent differentiation. Evolution is a dynamic force which leads to instability and absence of any perfection in the adaptive process. Natural selection acts mainly on phenotypes and only indirectly on the components of important breeding traits. In the northern part of the distribution of Scots pine there is a large among-and within-population variation in survival. The high amongpopulation variation occurs in spite of an assumed high gene flow. Biomass is a product of many components and it also shows a high population variation. Many markers are neutral and such markers will not reveal adaptive variation.

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“…This could be the case in L. delicatulum. A similar explanation has been suggested for ITS polymorphism in other plant species (Suh et al, 1993;Karvonen and Savolainen, 1993;Campbell et al, 1997).…”

Section: Intraspecific Variationmentioning

confidence: 78%

Study of the Evolutionary Relationships among Limonium Species (Plumbaginaceae) Using Nuclear and Cytoplasmic Molecular Markers

Palacios

Rosselló

Gónzález-Candelas

2000

Molecular Phylogenetics and Evolution

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The genus Limonium, due to the patchiness of the natural habitats of its species as well as the high frequency of hybridization and polyploidy and the possibility of reproduction by apomixis, provides an example of all the principal mechanisms of rapid speciation of plants. As an initial study of evolution in this genus, we have analyzed intra-and interspecific variability in 17 species from section Limonium, the largest in the genus, based on RFLPs of cpDNA and nuclear rDNA ITS sequences. In the cpDNA analysis, 21 restriction enzymes were used, resulting in 779 fragments, 490 of which were variable and 339 parsimony informative. L. furfuraceum exhibited two relatively divergent cpDNA haplotypes. The relationships found among the species based on cpDNA restriction fragments were coincident using different methods of phylogenetic analysis. Due to the presumed reticulate evolution in the genus Limonium, the comparison of these results with data from the nuclear DNA was necessary; ITS sequences were analyzed. The final alignment contained 488 characters, of which 198 were variable and 156 parsimony informative. Two relatively divergent ITS types were present at the intraindividual level in L. delicatulum, a triploid species. Each type was related to ITS from different groups of diploid Limonium species, one with a base haploid chromosome number n ‫؍‬ 8 (represented by L. cossonianum) and the other with n ‫؍‬ 9 (represented by L. minutum). The different phylogenetic inference methods used for the analysis of ITS sequences rendered very similar topologies. In general, the relationships among the species studied were coincident with those obtained with the chloroplast genome. Both nuclear and cytoplasmic markers support the polyphyly of section Limonium, with at least two species, L. narbonense and L. vulgare, clearly divergent from the rest. Moreover, the remaining subsections into which section Limonium is currently divided seem to be artificial.

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Variation and inheritance of ribosomal DNA in Pinus sylvestris L. (Scots pine)

Cited by 63 publications

References 38 publications

Intergenic spacer length variants in Old Portuguese bread wheat cultivars

Intergenic spacer length variants in Old Portuguese bread wheat cultivars

Evolutionary forces influencing variation among populations of Pinus sylvestris

Study of the Evolutionary Relationships among Limonium Species (Plumbaginaceae) Using Nuclear and Cytoplasmic Molecular Markers

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