Genetic effects of injection of Rous sarcoma virus DNA into polar plasm of early Drosophila melanogaster embryos

Gazaryan, K. G.; Nabirochkin, S D; Tatosyan, A. G.; Shakhbazyan, A. K.; Shibanova, E. N.

doi:10.1038/311392a0

Cited by 12 publications

(5 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These protocols exploit the capacity of insertion of mobile elements, particularly the P elements of Drosophila, used as tools to introduce new genetic information into the genomes. Fahmy & Fahmy, 1966Parkash, 1967Chopra, 1970Gershenson, Alexandrov & Maliuta, 1975Shandala & Gershenson, 1988Alexandrov & Golubovsky, 1983Burdette & Yoon, 1967Gazaryan et al, 1982Gazaryan et al, 1985 Unpublished data Tatosyan et al, Gazaryan et al, 1984Gazaryan et al, 1987 Unpublished data Baumiller, 1967 Unpublished data Golubovsky & Plus, 1982Rubin & Spradling, 1982Gazaryan et al, 1985Gray et al, 1992Bucala et al, 1993Sprague & McKinney, 1966Mottinger, Dellaporta & Keller, 1984Mottinger, Johns & Freeling, 1984Dellaporta et al, 1984Johns, Mottinger & Freefing, 1985Peterson, 1985 Unaffected genome structure ltaya, 1993…”

Section: Experiments With Environmental Factorsmentioning

confidence: 95%

Genome and stresses: Reactions against aggressions, behavior of transposable elements

Arnault

Dufournel

1994

Genetica

View full text Add to dashboard Cite

The action of stresses on the genome can be considered as responses of cells or organisms to external aggressions. Stress factors are of environmental origin (climatic or trophic) or of genomic nature (introduction of foreign genetic material, for example). In both cases, important perturbations can occur and modify hereditary potentialities, creating new combinations compatible with survival; such a situation may increase the variability of the genome, and allow evolutive processes to take place. The behavior of transposable elements under stress conditions is thus of particular interest, since these sequences are sources of mutations and therefore of genetic variability; they may play an important role in population adaptation. The survey of the available experimental results suggest that, although some examples of mutations and transposable elements movements induced by external factors are clearly described, environmental injuries or introduction of foreign material into a genome are not systematically followed by drastic genomic changes.

show abstract

Section: Experiments With Environmental Factorsmentioning

confidence: 95%

Genome and stresses: Reactions against aggressions, behavior of transposable elements

Arnault

Dufournel

1994

Genetica

View full text Add to dashboard Cite

show abstract

“…In Drosophila, visible mutations have been observed by Gazaryan et al (1982Gazaryan et al ( , 1984Gazaryan et al ( , 1985 after injection of the avian Rous Sarcoma Virus (RSV) and the Simian Virus SA7 into Drosophila embryos as particles or DNA. Because different loci were preferentially affected as a function of the origin of the invading virus, the authors hypothesized that endogenous transposable elements were implicated as intermediates of mutations.…”

Section: Introductionmentioning

confidence: 99%

Transposable elements behavior following viral genomic stress inDrosophila melanogaster inbred line

Jouan-Dufournel

Cosset²,

Contamine³

et al. 1996

J Mol Evol

View full text Add to dashboard Cite

To analyze the behavior of endogenous transposable elements under genomic stress, a Drosophila melanogaster inbred line was submitted to three kinds of viral perturbations. First, a retroviral plasmid containing the avian Rous Associated Virus type 2 (RAV-2) previously deleted for the viral envelope coding gene (env) was introduced by P element transformation into the Drosophila genome. An insertion of this avian retroviral sequence was detected by in situ hybridization in site 53C on polytene chromosome arm 2R. Second, Drosophila embryos were injected with RAV-2 particles produced by cell culture after transfection with the retroviral plasmid. Third, the Drosophila melanogaster inbred line was stably infected by the sigma native virus. It appears that neither the offspring of the flies in which the viral DNA was found integrated nor those from the infected sigma flies showed copia or mdg1 element mobilization. Injection of the avian RAV-2 particles led, however, to the observation of somatic transpositions of mdg1 element on the 2L chromosome, the copia element insertion pattern remaining stable. Thus, endogenous transposable elements show more instability in sublines injected with exogenous viral particles than in a transgenic subline containing a foreign viral insert, all transposable elements not being equally sensitive to such genomic stress.

show abstract

“…Higher rates in both excision and insertion in the natural population may be caused by fluctuations of the environment. The transposition of retrotransposons has been reported to be enhanced by environmental stress such as heat shock (Strand and McDonald 1985;Ziarczyk and Best-Belpomme 199 1;Ratner et al 1992) and virus infection (Gazaryan et al 1984). Nonrandom mutation rates have been observed in bacteria and yeast under certain nonlethal selections (see reviews of Foster 1993; Lenski and Mittler 1993).…”

Section: Resultsmentioning

confidence: 99%

Studies on the transposition rates of mobile genetic elements in a natural population of Drosophila melanogaster.

Suh

Choi²,

Yamazaki

et al. 1995

Molecular Biology and Evolution

View full text Add to dashboard Cite

In an isolated population of Drosophila melanogaster on Ishigaki Island the chromosomal distribution of several retrotransposons, including copia, 412, 297, 17.6, I, and jockey elements, was examined by in situ hybridization. In this population the cosmopolitan inversion, In(2L)t, is known to exist in high frequency. One major haplotype concerning the occupied sites of the transposable elements was identified in the In(2L)t-carrying chromosomes. This haplotype is suggested to be the ancestral one. The age of the inversion in this local population was estimated to be 1,400 generations. The transposition rates of these elements were estimated based on the age of the inversion and the number of the elements lost and gained. The excision rates were in the range from 9.13 x 10(-5) to 2.25 x 10(-4) per site per generation. They were similar each other in the copia-like elements as well as in the LINE-like elements. The rate was higher in the copia-like elements than in the LINE-like elements. Insertions occurred in the range from 6.79 x 10(-4) to 9.05 x 10(-4) per element per generation. It is herein shown that both insertions and excisions occurred at a significantly higher rate in this population than in the laboratory.

show abstract

Genetic effects of injection of Rous sarcoma virus DNA into polar plasm of early Drosophila melanogaster embryos

Cited by 12 publications

References 13 publications

Genome and stresses: Reactions against aggressions, behavior of transposable elements

Genome and stresses: Reactions against aggressions, behavior of transposable elements

Transposable elements behavior following viral genomic stress inDrosophila melanogaster inbred line

Studies on the transposition rates of mobile genetic elements in a natural population of Drosophila melanogaster.

Contact Info

Product

Resources

About