Genetic effects of Red Lettuce Leaf genes on red coloration in leaf lettuce under artificial lighting conditions

Abstract

Anthocyanins are commonly found in the plant kingdom and act as scavengers of reactive oxygen species produced by biotic and abiotic stress (Ahmed et al., 2015;Chalker-Scott, 1999;Liu et al., 2018). In the human diet, anthocyanins are functional food components that act as antioxidants and have anti-inflammatory effects (Pojer et al., 2013).Anthocyanin-rich crops are widespread and popular in supermarkets,

Deciphering transcriptomic signatures explaining the phenotypic plasticity of non-heading lettuce genotypes under artificial light conditions

“…Previously described methods that were modified slightly were used for the analysis (Arapitsas et al . 2008; Wada et al . 2022).…”

“…Our group reported that different combinations of RLL alleles result in quantitative differences in the accumulation of phytochemicals, especially anthocyanins, among lettuce cultivars under artificial light conditions (Wada et al 2022). Although the genetic variation has been confirmed, the differences due to multiple artificial light environments have not been demonstrated.…”

“…The relative contents of the following nine phytochemicals in lettuce extracts were determined: methods that were modified slightly were used for the analysis (Arapitsas et al 2008;Wada et al 2022). Powdered lettuce samples were extracted with methanol-water formic acid solution (40:59:1, v/v/v) for 1 h after 3 min sonication, followed by two extractions with 80% (v/v) methanol.…”

“…Our group reported that different combinations of RLL alleles result in quantitative differences in the accumulation of phytochemicals, especially anthocyanins, among lettuce cultivars under artificial light conditions (Wada et al . 2022).…”

“…A previous study identified four genes (RLL1-RLL4) that modulate the anthocyanin (cyanidin-glycoside) content in lettuce(Su et al 2020). Moreover, we previously showed that a mutated RLL4 allele (rll4), which is a RUP gene related to the negative feedback regulation of UV-B signaling(Gruber et al 2010;Su et al 2020), promotes cyanidin-glycoside and quercetin-glucoside accumulation under fluorescent lights(Wada et al 2022). Therefore, we compared these phytochemical contents among lettuce cultivars with different RLL4 alleles under four artificial light and UV-B-supplemented conditions.…”