Genetic effect of anatase on osteoblast‐like cells

Abstract

Titanium is the gold standard among materials used for prosthetic devices, because of its good mechanical and chemical properties. When exposed to oxygen, titanium becomes an oxide that is biocompatible and able to induce osseointegration. Three allotropic forms of titanium dioxide exist, that is brookite, rutile, and anatase. Anatase can be prepared as a colloidal suspension and then used to coat surfaces. Anatase coating (AC) can potentially have specific biological effects. Here we are testing the effect of AC on osteoblast-like cells (MG63) by using microarray techniques to identify genes that are differently regulated in osteoblasts exposed to AC. By using DNA microarrays containing 20,000 genes, we identified in osteoblast-like cell lines (MG-63) cultured on AC, several genes whose expression was significantly up- or downregulated. They cover a broad range of functional activities: signaling transduction, immunity, cell cycle regulation, lysosomes composition and vesicular transport, cell adhesion, cytoskeleton and extracellular matrix components, proliferation, and apoptosis. The data reported constitute, to our knowledge, the first genetic portrait of AC effects. They can be relevant to a better understanding of the molecular mechanism of bone regeneration and as a model for comparing other materials with similar clinical effects.

Application-wise nanostructuring of anodic films on titanium: a review

“…[59À61] This effect is due to increased bone growth and decreased bacteria proliferation compared to the bare metal: as reported by Sollazzo et al, [62] anatase specifically regulates some gene expressions related to immunity which suppress foreign body recognition, which is critical in the grafting process. Anatase was also observed to act in vesicular transport, apoptosis and cell cycle regulation, producing modifications in the turnover of extracellular matrix components and in osteoblast proliferation.…”

Alveolar ridge augmentation with calvaria, iliac crest and mandibular autologous bone grafts: a retrospective study on 261 implants

“…The minidesign is the dimension of the threads or the shape of the neck (the dimension ranged from 1 to 0.1 mm); the microdesign is the shape of implant's surface (for example the "grooves and holes" due to surface treatments like machination, acid etching and sand-blasting procedures). Finally, the nanodesign is determined by the molecular composition of the surface [21][22][23][24]. Thus it is not surprising that different types of fixtures have different impact on bone resorption ( Table 2).…”

“…From a general point of view it is possible to distinguish the macro-, mini-, micro-and nano-design [21][22][23][24] for each implant type. The macrodesign is the shape of the implant, for example cylindrical or root form.…”

Trabecular Titanium Induces Osteoblastic Bone Marrow Stem Cells Differentiation

“…The minidesign is related to the featured dimensions of the prosthesis, like indentations, threads and fins. The microdesign is the shape of the implant surface, due to surface treatments like machining, acid etching and sand-blasting procedures that determine the surface roughness, characterized by a cellular dimension [19]. Finally, the nanodesign is determined by the molecular composition of the surface.…”