The genetic diversity of 50 sesame genotypes was studied by Mahalanobis D 2 statistics. By using Tocher's method, genotypes were grouped into nine clusters based on 10 economically important traits viz., days to flowering, days to 50% flowering, days to maturity, plant height, the number of primary branches, the number of secondary branches, the number of capsules per plant, the number of seeds per capsules, thousand seed weight and single plant yield. The largest intercluster distance among the nine clusters was found between clusters I and VIII, indicated that they were significantly more diverse and clusters IV and VI showed the lowest intercluster distance indicating that the genotypes under these clusters are closely related. The cluster with the highest cluster mean was found in cluster VIII for the number of capsules per plant, the number of primary and secondary branches, the number of seeds per capsule and single plant yield and cluster V for plant height and cluster VII for thousand seed weight. Taking into account the highest cluster mean for a trait, genotypes could be selected as parents in the hybridization program to create superior cross combinations.