Genetic diversity of wild and domesticated stocks of Thai abalone, Haliotis asinina (Haliotidae), analyzed by single-strand conformational polymorphism of AFLP-derived markers

Praipue, P.; Klinbunga, Sirawut; Jarayabhand, Padermsak

doi:10.4238/vol9-2gmr808

Cited by 9 publications

(13 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Surprisingly, patterns of genetic differentiation (e.g., between pairs of geographic samples) based on RAPD analysis did not indicate long range migration of P. pelagicus in our study, and the patterns were different from those of other marine species locally found in Thai waters, including P. monodon (Khamnamtong et al, 2009), P. merguiensis (Hualkasin et al, 2003), H. asinina (Praipue et al, 2010) and H. varia (Klinbunga et al, 2003); in these previous studies, significant genetic heterogeneity was found between coastal regions (i.e., between the Andaman Sea and the Gulf of Thailand).…”

Section: Discussionmentioning

confidence: 38%

Genetic population differentiation of the blue swimming crab Portunus pelagicus (Portunidae) in Thai waters revealed by RAPD analysis

Klinbunga¹,

Yuvanatemiya²,

Wongphayak³

et al. 2010

Genet. Mol. Res.

Self Cite

View full text Add to dashboard Cite

ABSTRACT. Genetic diversity and population differentiation of the blue swimming crab, Portunus pelagicus, in Thailand were analyzed by RAPD analysis. One hundred and twelve RAPD fragments were generated from 109 individuals of P. pelagicus using OPA02, OPA14, OPB10, UBC122, and UBC158 primers. The percentage of polymorphic bands in each geographic sample and that of each primer across overall samples were 72.7-85.0 and 92.0-100%, respectively. Large numbers of polymorphic bands found in the RAPD analysis suggested high genetic diversity of Thai P. pelagicus. The mean genetic distance between samples across all primers was 0.0929-0.2471. Significant geographic heterogeneity was observed across samples overall and between all pairs of geographic samples (P < 0.01 for θ and P < 0.0001 for the exact test), indicating strong genetic differentiation of P. pelagicus in Thai waters, despite its high potential of dispersal. Limited gene flow levels (0.44-1.19 individuals per generation) of Thai P. pelagicus were also observed. A fine scale level of differentiation suggested that P. pelagicus from each geographic sample in Thai waters should be regarded as a separate genetic population and treated as a different exploited stock.

show abstract

Section: Discussionmentioning

confidence: 38%

Genetic population differentiation of the blue swimming crab Portunus pelagicus (Portunidae) in Thai waters revealed by RAPD analysis

Klinbunga¹,

Yuvanatemiya²,

Wongphayak³

et al. 2010

Genet. Mol. Res.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Knowledge of the genetic diversity of P. pelagicus is essential for the conservation and aquaculture of this important crab resource, in order to construct an appropriate management scheme (Bryars and Adams, 1999). The genetic population structure of P. pelagicus has been investigated based on allozymes (Bryars and Adams, 1999), cytochrome oxidase subunit I (COI), microsatellite markers (Sezmis, 2004), amplified fragment length polymorphisms (Praipue et al, 2010), and random amplification of polymorphic DNA .…”

Section: Introductionmentioning

confidence: 99%

Characterization of the complete mitochondrial genome of Portunus pelagicus with implications for phylogenomics

Ren

et al. 2016

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. This study determined the mitochondrial genome structure of the blue swimming crab (Portunus pelagicus), and elucidated its phylogenetic relationships among the species within the order Decapoda. The complete mitochondrial genome was 16,155 bp long, and contained 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and 1 DNA control region. The gene order of the genome was the same as that found within the family Portunidae. Twenty-three genes were on the heavy strand and 14 were on the light strand. Almost all of the protein-coding genes were initiated by an ATG codon, except for three genes (ATP6, ND1, and ND3) that started with a rare ATT codon. Of the 13 protein-coding genes, 10 ended with complete TAA or TAG stop codons and three ended with an incomplete T codon. Thirteen non-coding regions were identified that ranged from 1 to 30 bp in length. Nine overlaps were found, which ranged from 1 to 7 bp in length. Phylogenetic analyses based on 12 concatenated protein-coding genes revealed that P. pelagicus formed a monophyletic group with Portunus trituberculatus, which were in a larger group with Callinectes sapidus, while the genera Charybdis and Thalamita formed another group. These two groups clustered together and grouped with the genus Scylla. The phylogenetic analysis supported the inclusion of Charybdis in subfamily Portuninae of the family Portunidae, and revealed a close relationship between Charybdis and Thalamita. We suggest that Thalamita should also be classified into the subfamily Portuninae. The results can be used in the study of phylogenetic, population genetic and conservation genetics of P. pelagicus.

show abstract

“…This implies that within‐population genetic variability can be low, but total genetic variability could remain unaffected (Falconer & Mackay, ), although out of the H‐W equilibrium due to the Wahlund effect and inbreeding. Aquaculture usually induces changes in the populations genetic structure and diversity due to founder effects, low effective number (Ne) of brooders, differences in genetic contribution of brooders in the reproductive process and domestic selection (Hedgecock & Sly, ; Li, Shu, Yu, & Tian, ; Liu, Zeng, Du, & Rao, ; Praipue, Klinbunga, & Jarayabhand, ; Rhode et al, ; Verspoor, , among others). As a consequence, the genetic pool of wild populations might be negatively affected when populations generated through aquaculture are used for restocking wild populations, or gametes admixture occurs due to both wild and aquaculture populations share the same environment (Beaumont, ; Harada, Yokota, & Iizuka, ; Ryman, Jorde, & Laikre, ; Ryman & Laikre, ; Waples, Hindar, Karlsson, & Hard, ).…”

Section: Discussionmentioning

confidence: 99%

Assessing the genetic diversity in Argopecten nucleus (Bivalvia: Pectinidae), a functional hermaphrodite species with extremely low population density and self‐fertilization: Effect of null alleles

Barros

Winkler

Velasco

2020

Ecology and Evolution

View full text Add to dashboard Cite

Argopecten nucleus is a functional hermaphroditic pectinid species that exhibits self‐fertilization, whose natural populations have usually very low densities. In the present study, the genetic diversity of a wild population from Neguanje Bay, Santa Marta (Colombia), was estimated using microsatellite markers, and the effect of the presence of null alleles on this estimation was assessed. A total of 8 microsatellite markers were developed, the first described for this species, and their amplification conditions were standardized. They were used to determine the genotype of 48 wild individuals from Naguanje Bay, and 1,010 individuals derived from the offspring of 38 directed crosses. For each locus, the frequencies of the identified alleles, including null alleles, were estimated using the statistical package Micro‐Checker, and the parental genotypes were confirmed using segregation analysis. Three to 8 alleles per locus with frequencies from 0.001 to 0.632 were detected. The frequencies of null alleles ranged from 0.10 to 0.45, with Ho from 0.0 to 0.79, and He from 0.53 to 0.80. All loci were in H‐W disequilibrium. The null allele frequencies values were high, with lower estimations using segregation analysis than estimated using Micro‐Checker. The present results show high levels of population genetic diversity and indicate that null alleles were not the only cause of deviation from H‐W equilibrium in all loci, suggesting that the wild population under study presents signs of inbreeding and Wahlund effect.

show abstract

Genetic diversity of wild and domesticated stocks of Thai abalone, Haliotis asinina (Haliotidae), analyzed by single-strand conformational polymorphism of AFLP-derived markers

Cited by 9 publications

References 37 publications

Genetic population differentiation of the blue swimming crab Portunus pelagicus (Portunidae) in Thai waters revealed by RAPD analysis

Genetic population differentiation of the blue swimming crab Portunus pelagicus (Portunidae) in Thai waters revealed by RAPD analysis

Characterization of the complete mitochondrial genome of Portunus pelagicus with implications for phylogenomics

Assessing the genetic diversity in Argopecten nucleus (Bivalvia: Pectinidae), a functional hermaphrodite species with extremely low population density and self‐fertilization: Effect of null alleles

Contact Info

Product

Resources

About