Genetic population differentiation of the blue swimming crab Portunus pelagicus (Portunidae) in Thai waters revealed by RAPD analysis

Abstract

ABSTRACT. Genetic diversity and population differentiation of the blue swimming crab, Portunus pelagicus, in Thailand were analyzed by RAPD analysis. One hundred and twelve RAPD fragments were generated from 109 individuals of P. pelagicus using OPA02, OPA14, OPB10, UBC122, and UBC158 primers. The percentage of polymorphic bands in each geographic sample and that of each primer across overall samples were 72.7-85.0 and 92.0-100%, respectively. Large numbers of polymorphic bands found in the RAPD analysis suggested high genetic diversity of Thai P. pelagicus. The mean genetic distance between samples across all primers was 0.0929-0.2471. Significant geographic heterogeneity was observed across samples overall and between all pairs of geographic samples (P < 0.01 for θ and P < 0.0001 for the exact test), indicating strong genetic differentiation of P. pelagicus in Thai waters, despite its high potential of dispersal. Limited gene flow levels (0.44-1.19 individuals per generation) of Thai P. pelagicus were also observed. A fine scale level of differentiation suggested that P. pelagicus from each geographic sample in Thai waters should be regarded as a separate genetic population and treated as a different exploited stock.

Genetic characterization and evaluation of anthropogenic impacts on genetic patterns in cultured and wild populations of mussels (Mytilus galloprovincialis) from Greece

“…RAPD is a low cost, rapid method that can be performed without any prior nucleotide sequence knowledge of the target DNA. The latter advantage has made the RAPD technique a very useful tool for studying the genetics of non-model species, in which little genome information is available (Klinbunga et al, 2010). Its simplicity and rapidity is mainly due to the use of a single, short and arbitrary oligonucleotide primer for amplification of the template DNA, thus avoiding the need for expensive and sophisticated equipment (Bardakci, 2001).…”

Two genetic stocks of Steindachneridion melanodermatum living in sympatry in nature and genetic variability of wild parents and F1 generation

“…In other groups, RAPD Genetic variability in Steindachneridion melanodermatum markers have been combined with methodologies of a less arbitrary nature in order to generate better defined DNA patterns, i.e., DNA fingerprinting, for the purpose of molecular characterization (Aggarwal et al, 2008;Demir et al, 2010), for analysis of genetic diversity (Brahmane et al, 2008;Rabello et al, 2008) to provide practical information for selection of parentals in planning breeding strategies (Ahmad et al, 2010), and to determine phylogenetic relationships (Marouelli et al, 2010) besides population differentiation (Klinbunga et al, 2010) between wild and cultivated stocks (Sesli and Yegenoglu, 2010). Although the RAPD method does not provide accurate information regarding the specific genotype of the specimens analyzed or the dominant character of the marker, it is a fast, inexpensive tool that allows access to information on general genetic variability within and between populations, even when considering that the observed variation may be underestimated.…”

Population genetic structure of the freshwater anomuran Aegla uruguayana Schmitt, 1942 (Decapoda, Aeglidae) in the central region of Argentina

“…In freshwater decapods, population genetic evidence studies suggest different patterns that indicate structural levels according to distance or population separa tion in time or space, or variations according to habitat area, habitat age, and the immigration potential of the locality (Fuller & Lester, 1980;Daniels et ah, 1999;Daniels, 2003;Schubart & Huber, 2006;Shih et al, 2006;Xu et al, 2009;Klinbunga et al, 2010;Barber et al, 2011Barber et al, , 2012.…”