Genetic diversity of viruses associated with sugarcane mosaic disease of sugarcane inter-specific hybrids in China

Abstract

In this study, we investigated 78 sugarcane samples with severe mosaic symptoms collected from four provinces of southern China: Guangxi, Yunnan, Hainan, and Guangdong, which cover nearly 85 % of commercial sugarcane planting zones in China. Using RT-PCR, sequencing and phylogenetic analysis, we identified 72 hybrid sugarcane samples containing causal agents of sugarcane mosaic disease. Among these, 66 virus isolates were identified as Sorghum mosaic virus (SrMV) (84.6 %), four were identified as Sugarcane streak mosaic virus (SCSMV) (5.1 %), and two were identified as Sugarcane mosaic virus (SCMV) (2.6 %). The isolates of SrMV were classified into three subgroups: I, II, and III with a 95 % similarity level. The two largest subgroups, SrMV I containing 36 sugarcane mosaic diseases isolates (46.2 %), and SrMV III containing 20 isolates (25.6 %), were prevalent in Guangdong and Guangxi provinces; however, SrMV II containing five isolates (6.4 %) did not exhibit any close association with geographical distribution. The popular sugarcane cultivar, ROC22, was found Eur J Plant Pathol

Genetic diversity and population structure of Sorghum mosaic virus infecting Saccharum spp. hybrids

“…hybrids with mosaic symptoms collected from Chinese sugarcane‐growing locations. Similar results have been reported by Zhang et al (). However, previous studies documented that SCMV was equally important to SrMV causing mosaic disease in Saccharum spp.…”

“…However, the three isolates, together with five isolates from Argentina and the USA, were clustered into a small subgroup in SrMV‐G4 in this study, underscoring the importance of obtaining enough sequenced samples for SrMV phylogenetic grouping. Clustering for Chinese SrMV isolates has been investigated by Wang et al () and Zhang et al (), which led to the proposal of three SrMV groups (I, II and III). When considering the previously reported isolates together with those from this study, all Chinese SrMV isolates cluster into six distinct phylogroups, with the SrMV‐G5 group being the first reported here.…”

“…Differentiation of SCMV and SrMV strains was possible using reverse transcriptase‐polymerase chain reaction (RT‐PCR)‐based restriction fragment length polymorphism (Yang & Mirkov, ). Since then, the diversity of many isolates/strains of SCMV or SrMV that infect sugarcane has been investigated on the basis of the NIb or CP sequences in Australia (Handley et al , ), USA and Southern Africa (Handley et al , ), Mexico (Espejel et al , ), Brazil (Gonçalves et al , ), Argentina (Perera et al , ), Cameroon and Congo (Alegria et al , ), Thailand (Gemechu et al , ), India (Viswanathan et al , ), China (Xu et al , ; Wang et al , ; Zhang et al , ), Vietnam (Ha et al , ) and Pakistan (Haider et al , ).…”

“…hybrids and S. officinarum cultivars in Yunnan (YN) province, China, revealed that most of the SCMV isolates formed a distinctive cluster together with three Vietnamese isolates, whereas the SrMV isolates grouped into three major clusters (Wang et al , ). Zhang et al () recently reported 66 SrMV isolates obtained from four provinces in China that were classified into subgroups I, II and III with a 95% similarity level.…”

“…The viruses and their associated strains that cause mosaic in commercial sugarcane cultivars ( Saccharum spp. hybrid) in China have not been clearly identified, although some preliminary results have been reported in limited sugarcane‐growing areas (Xu et al , ; Wang et al , ; Zhang et al , ). Therefore, in this study, we conducted extensive surveys and obtained 104 leaf samples with mosaic symptoms in sugarcane plants ( Saccharum spp.…”