Genetic diversity of Trypanosoma evansi in beef cattle based on internal transcribed spacer region

Areekit, Supatra; Singhaphan, Pirom; Kanjanavas, Pornpimon; Khuchareontaworn, Sintawee; Sriyapai, Thayat; Pakpitcharoen, Arda; Chansiri, Kosum

doi:10.1016/j.meegid.2007.10.008

Cited by 24 publications

(20 citation statements)

References 14 publications

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“…The present study has employed sequencing and phylogenetic tree construction based on the rDNA complete ITS region to evaluate intraspecific diversity of T. evansi from water buffaloes in the Philippines. This region has been generally used in assessing genetic variation in a number of protozoans including T. evansi (Khuchareontaworn et al 2007, Tian et al 2011, Areekit et al 2008, Amer et al 2011. Other DNA targets that were used to distinguish T. evansi interspecifically and intraspecifically to date include 18S rDNA, kinetoplast DNA, microsatellite sequences, and the expression-site-associated genes (ESAGs) (Biteau et al 2000, Witola et al 2005, Nijru et al 2007).…”

Section: Resultsmentioning

confidence: 99%

“…Other DNA targets that were used to distinguish T. evansi interspecifically and intraspecifically to date include 18S rDNA, kinetoplast DNA, microsatellite sequences, and the expression-site-associated genes (ESAGs) (Biteau et al 2000, Witola et al 2005, Nijru et al 2007). Nevertheless, only ESAG6 and rDNA complete ITS region were proven to be useful genetic markers for classification within this species (Witola et al 2005, Khuchareontaworn et al 2007, Areekit et al 2008). In the study by Mekata et al 2009, phylogenetic analysis of T. evansi ESAG6 yielded 10 clades, 6 of which contain Philippine isolates (not the same isolates used in the present study).…”

Section: Resultsmentioning

confidence: 99%

“…Final extension was done at 72ºC for 7 min ). Amplification of T. evansi rDNA complete ITS region was done using the primers ITS/F and ITS/R, which generate amplicons of ~1,300 bp (Khuchareontaworn et al 2007, Areekit et al 2008). The amplification conditions followed were as described by Khuchareontaworn et al 2007, which were set to give an initial denaturation at 94ºC for 2 min, followed by 30 cycles each of denaturation at 94ºC for 1 min, annealing at 60ºC for 1 min, and extension at 72ºC for 1 min.…”

Section: Pcr Amplification Was Carried Out Using I-taqmentioning

confidence: 99%

“…In fact, this marker has been very useful in molecular analysis to establish relationships among and within trypanosome species such as T. b. brucei, T. b. gambiense, T. cruzi, T. rangeli, and T. evansi (Agbo et al 2001, Santos et al 2002, Beltrame-Botelho et al 2005, Khuchareontaworn et al 2007, Areekit et al 2008 *Corresponding author: wlrivera@science.upd.edu.ph Trypanosoma evansi isolates from the Philippines 7 2011). Previous study based on ITS regions of T. brucei has established genotypic discrimination between its two subspecies (Agbo et al 2001).…”

Section: Introductionmentioning

confidence: 99%

“…Previous study based on ITS regions of T. brucei has established genotypic discrimination between its two subspecies (Agbo et al 2001). In T. evansi species, recent investigations on ITS sequences provided evidence on the intraspecific differentiation of T. evansi within a single host and from several hosts (Khuchareontaworn et al 2007, Areekit et al 2008, Amer et al 2011. This information on the genetic diversity of T. evansi can be used for epidemiological studies specifically in assessing geographical distribution, transmission dynamics, and virulence pattern of the parasite for the prevention and/or management of the disease that it causes particularly during outbreaks.…”

Section: Introductionmentioning

confidence: 99%

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Molecular characterization of Trypanosoma evansi isolates from water buffaloes (Bubalus bubalis) in the Philippines

Villareal

Mingala

Rivera³

2013

Acta Parasitologica

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Trypanosoma evansi infection in the Philippines is frequently reported to affect the country's livestock, particularly, the buffaloes. To assess the prevalence and intraspecific diversity of T. evansi in the country, blood samples from water buffaloes in different geographical regions were collected during an outbreak. T. evansi was detected in all 79 animals tested using PCR targeting the RoTat 1.2 VSG gene. Sequencing of the rDNA complete internal transcribed spacer (ITS) region including the 5.8S subunit showed high similarity (99-100%) between Philippine isolates and known T. evansi isolates in Genbank. Tree construction based on the same region confirmed the close relationship between Philippine and reported Thai isolates as compared to Egyptian isolates separated by relatively small genetic distances, 47 polymorphisms, despite the clustering in four branches. Overall, the results of this study prove genetic diversity within T. evansi species despite previous reports on limited heterogeneity among isolates worldwide.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Pcr Amplification Was Carried Out Using I-taqmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Molecular characterization of Trypanosoma evansi isolates from water buffaloes (Bubalus bubalis) in the Philippines

Villareal

Mingala

Rivera³

2013

Acta Parasitologica

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Genetic characterization and phylogenetic analysis of Trypanosoma evansi in Iranian dromedary camels

et al. 2012

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Whole blood samples were collected from 117 male clinically healthy Camelus dromedarius aged between 6 months to 18 years from several farms in Yazd Province of Iran. Trypanosoma evansi-affected camels were detected by Giemsa-stained blood smears, and the positive blood samples (4 out of 117) were submitted to PCR examination and phylogenetic analysis. Basic Local Alignment Search Tool data of the obtained complete internal transcribed spacer (ITS) sequences revealed that they corresponded to those of T. evansi, Thailand cattle isolate (AY912276) with the homology of 99 %. Both phylogenetic trees generated by ITS1 and complete ITS were unable to clearly show inter- and intraspecific genetic diversity of Trypanosoma spp. isolates. The phylogenetic tree inferred from the ITS2 nucleotide sequences (569 bp) clearly showed the genetic diversity of the parasites. Phylogenetic and molecular analyses of this region showed that two distinct genotypes of T. evansi in Iranian dromedary camels are present. In contrast to the ITS1 and ITS2 regions, multiple alignment of the nucleotide sequence of the 5.8S rRNA showed a high degree of sequence conservation during evolution in various Trypanosoma spp.

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Assessment of genetic diversity of Trypanosoma evansi in the domestic animal populations through ITS-1 gene sequence analysis

Verma,

Punia,

Das

et al. 2023

Parasitol Res

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Genetic diversity of Trypanosoma evansi in beef cattle based on internal transcribed spacer region

Cited by 24 publications

References 14 publications

Molecular characterization of Trypanosoma evansi isolates from water buffaloes (Bubalus bubalis) in the Philippines

Molecular characterization of Trypanosoma evansi isolates from water buffaloes (Bubalus bubalis) in the Philippines

Genetic characterization and phylogenetic analysis of Trypanosoma evansi in Iranian dromedary camels

Assessment of genetic diversity of Trypanosoma evansi in the domestic animal populations through ITS-1 gene sequence analysis

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