Genetic Diversity of Rhubarb Cultivars

Abstract

<jats:p>The genus <jats:italic>Rheum</jats:italic> L., commonly known as rhubarb, is composed of ≈60 species, primarily distributed throughout northern and central Asia. Rhubarb species have been used for medicinal purposes for thousands of years; however, it was not until the 18th century that the culinary use of petioles was first reported. Although the origin(s) of culinary rhubarb is not clear, it is thought that they originated from hybridization of rhubarb species originally brought to Europe for medicinal purposes. Most rhubarb cultivars lack pedigree information, and the genetic relationship among cultivars is largely unknown. Amplified fragment length polymorphism (AFLP) markers were generated for fingerprint analysis of 37 cultivars and four putative <jats:italic>Rheum</jats:italic> species accessions. Ten <jats:italic>Eco</jats:italic>RI and <jats:italic>Mse</jats:italic>I primer combinations were analyzed for a total of 1400 scored polymorphisms, with an average of 140 polymorphisms per primer combination. Results show at least two clusters of related cultivars, as well as distantly related accessions. This study provides an estimate of rhubarb cultivar genetic diversity using AFLP analysis.</jats:p>

Short-read DNA Sequencing Yields Microsatellite Markers for Rheum

“…Contamination from seedlings could explain the genetic variation we observed among these similarly named accessions. These results agree with Kuhl and DeBoer's (2008) suggestion that the two 'German Wine' or 'Prince Albert' or 'Crimson Red' accessions could have arisen from seedlings. Mislabeling could also have occurred during exchange of these rhubarb clones.…”

“…The majority of the well-supported groups (72% or greater bootstrap support) in this study was also well-supported based on AFLP analysis of 37 culinary rhubarb accessions and four species representatives (greater than 90% bootstrap support; Kuhl and DeBoer, 2008), suggesting close genetic relationships. These five well-supported groups were named following Kuhl and DeBoer (2008) and include: 1) 'Cherry Red'; 2) 'Canada Red'; 3) 'Parson's Crimson'; 4) 'Valentine'; and 5) 'Sutton'. Three additional groups with strong bootstrap support were also observed: 'Ben Vanderweel' (72%), 'MacDonald' (74%), and 'Coulter MacDonald' (82%) (Fig.…”

“…Bootstrap support based on 1000 iterations is indicated above each branch if it exceeded 50%. Five groups ('Cherry Red', 'Canada Red', 'Parson's Crimson', 'Valentine', and 'Sutton') with bootstrap support 72% or greater were named following Kuhl and DeBoer (2008) in the amplified fragment length polymorphism (AFLP)-based unweighted pair group method with arithmetic mean (UPGMA) dendrogram. Three additional groups with strong bootstrap support were also observed: 'Ben Vanderweel' (72%), 'MacDonald' (74%), and 'Coulter MacDonald' (82%).…”

“…A few molecular marker studies have been reported in rhubarb. Kuhl and DeBoer (2008) used AFLP markers to investigate the genetic diversity of 41 accessions from the Palmer Rheum collection. Hu et al (2010Hu et al ( , 2011 used intersimple sequence repeat (ISSR) markers to assay genetic diversity in Chinese populations of R. tanguticum, whereas Wang (2011) optimized ISSR-polymerase chain reaction in R. officinale, R. palmatum, and R. tanguticum.…”

Keragaan Pertumbuhan Kelembak Dari Berbagai Daerah Di Jawa Tengah

“…berasal dari daratan Tengah China kemudian menyebar ke Eropa dan daerah sub tropik lainnya (Kuhl & DeBoer, 2008 Akar kelembak mengandung aloe-imodin, rhein, emodin dan physcion (Zhao N, et al, 2014), anthrones dan glikosida, stilbenes, sakarida, tanin dan sebagainya (Sheng, et al, 2011). Dalam beberapa tahun terakhir ditemukan komponen baru seperti sulfemodin8-O-b-D-glukosida, revandchinone-1, revandchinone-2, revandchinone-3, revandchinone-4, 6-methylrhein dan 6-methyl aloe-emodin (Ahmad, et al, 2013).…”