Genetic Diversity of Plain Medium Resistant of Chrysanthemum Planlet Produced of Gamma Ray Irradiation

Abstract

Genetic diversity can be improved using the gamma ray irradiation to obtain the resistant plants to the disease. The Changes occur can be analyzed at the beginning of plant growth using molecular analysis; RAPD technique (Random Amplified Polymorfism DNA). This study aims to determine the genetic diversity produced by gamma rays irradiated and given the poly ethilene glycol given in plantlets to increase chrysanthemum resistance to Medium plain. The study used four primers tested on four treatments that were not irradiated plantlets and induced the poly ethilene glycol, gamma rays irradiated 25 gray un induced poly ethilene glycol, irradiated and induced the poly ethilene glycol 15 and 30%.The results showed a decrease of control diversity of genetic 0.20 to 0.30 in 25 gray irradiation treatment with given the poly ethilene glycol 15%.The use of the RAPD technique to determine genetic diversity resulting from gamma ray irradiation on the level of plantlets. Gamma ray irradiation treatment causing mutations in all treatments tested. Plantlets of 25 gray irradiation induced with 15%poly ethylene glycol / l showed changes in DNA structure.

Effect of Charcoal Type and Saccharose Concentration on The Growth of Abaca Banana Root (Musa Textilis Nee.)

“…In terms of root length, the treatment using activated charcoal norit showed significantly longer roots than other kinds of activated charcoal because the activated charcoal in norit could reduce the light coming into the growing medium. Notably, pro-analysis activated charcoal and norit contained a higher percentage of activated charcoal, approximately 99 percent, while plant-derived activated charcoal contained 95-99 percent [1,8]. On the other hand, activated charcoal derived from coconut shells showed better overall performance than plant-derived activated charcoal, although it was still lower than norit.…”

“…Sugar exists in large amounts in sweet fruits, stems, roots, tubers, and the sap of some higher plants, where saccharose is soluble in water. Saccharose functions in the thickening of cell walls, while auxin is a catalyst in the metabolism of carbohydrates and in regulating mitosis activity [1,18]. Wijayani and Muafi [11] conducted studies on Chrysanthemum plant tissue culture using ½ MS medium, and 40 g/l saccharose showed faster growth.…”

“…The Abaca plant is a species of banana with high product value and general uses, such as being used as a textile material and a material for papers for important documents. Abaca is an emerging commodity with limited availability to meet the market demand, necessitating extensive cultivation [1,2,3]. The economic value of the Abaca plant lies in its stem, which contains fibers as the raw materials of textiles and papers.…”

Acclimatization of Chrysanthemum Plantlet after Gamma Ray Irradiation

“…This mutation was closely related to the dosage of gamma rays applied [11]. In another study conducted by Wijayani and Amiaji [9] that used colchicine, there was a change in the color into yellowish green, which is assumed to be caused by the application of too little concentration of colchicine. According to Chandler and Brugliera [12], colchicine will be effective in the concentration of 0.1 -0.8 percent, with soaking time of 24-96 hours.…”