Genetic diversity of Mycoplasma arginini isolates based on multilocus sequence typing

Olaogun, Olusola Martins; Kanci, Anna; Barber, Stuart; Tivendale, Kelly A.; Markham, Philip F.; Marenda, Marc S.; Browning, Glenn F.

doi:10.1016/j.vetmic.2015.07.028

Cited by 15 publications

(20 citation statements)

References 20 publications

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“…Since previous studies reported phylogenetic differences between isolates coming from the same farms [16, 17], our study included the study of three farms from which more than one isolate were retrieved. Phylogenetic differences appeared between different isolates coming from one of these farms, but only when isolates were retrieved at different years.…”

Section: Resultsmentioning

confidence: 99%

“…In connection with previous studies performed on other mycoplasma species which MLST schemes showed genetic diversity between isolates coming from the same flocks [16, 17], in the present work, different strains isolated from the same herds were selected in order to compare them. In this context, when isolates were taken at different years, phylogenetic differences were shown, highlighting the genetic variability of this pathogen.…”

Section: Discussionmentioning

confidence: 99%

“…This result is contrary to the outcomes reported for M . arginini [16] and Mycoplasma mycoides subsp. capri [17], which showed significant differences between the MLST analyses of different strains isolated in the same conditions.…”

Section: Discussionmentioning

confidence: 99%

“…For this purpose, several typing techniques have been developed so as to compare isolates, including multilocus sequence typing (MLST). This technique utilizes sequences of internal fragments from housekeeping genes in order to characterize bacterial isolates, and it seems to be the most appropriate technique to analyze evolutionary relationships amongst mycoplasma isolates [13–16]. Hence, in previous studies with other mycoplasmas, a great genetic diversity between isolates from the same area (country) [13, 17] or even from the same farm [16] was observed.…”

Section: Introductionmentioning

confidence: 99%

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Antimicrobial susceptibility and multilocus sequence typing of Mycoplasma capricolum subsp. capricolum

Tatay-Dualde

Ham

et al. 2017

PLoS ONE

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Mycoplasma capricolum subsp. capricolum is one of the causative agents of contagious agalactia (CA). Nevertheless, there is still a lack of information about its antimicrobial susceptibility and genetic characteristics. Therefore, the aim of this work was to study the antimicrobial and genetic variability of different Mycoplasma capricolum subsp. capricolum field isolates. For this purpose, the growth inhibition effect of 18 antimicrobials and a multilocus sequence typing (MLST) scheme based on five housekeeping genes (fusA, glpQ, gyrB, lepA and rpoB) were performed on 32 selected field isolates from Italy and Spain.The results showed a wide range of growth inhibitory effects for almost all the antimicrobials studied. Macrolides presented lower efficacy inhibiting Mcc growth than in previous works performed on other CA-causative mycoplasmas. Erythromycin was not able to inhibit the growth of any of the studied strains, contrary to doxycycline, which inhibited the growth of all of them from low concentrations. On the other hand, the study of the concatenated genes revealed a high genetic variability among the different Mcc isolates. Hence, these genetic variations were greater than the ones reported in prior works on other mycoplasma species.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antimicrobial susceptibility and multilocus sequence typing of Mycoplasma capricolum subsp. capricolum

Tatay-Dualde

Ham

et al. 2017

PLoS ONE

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“…Phylogenetic analysis of rpoB sequences demonstrated higher sequence identity between Indian isolates irrespective of geographical origin in contrast to remarkable genetic variability among Victorian isolates by MLST (14). Based on biochemical and molecular tests, 5.3% prevalence of M. arginini was identified on screening 244 caprine pneumonia lung samples, which is in fact slightly less but surely gives an insight into its seriousness.…”

Section: Isolatesmentioning

confidence: 90%

Mycoplasma arginini: high frequency involvement in goat pneumonia

Valsala¹,

Rana²,

Remesh³

et al. 2017

Turk J Vet Anim Sci

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Mycoplasmas are an important class of bacteria associated with caprine pneumonia. Some of the main species involved are M. mycoides subsp. capri, M. capricolum subsp. capricolum, and M. capricolum subsp. capripneumoniae. In addition, there are frequent reports of M. arginini involvement. In the present study an attempt was made to see the association of various mollicutes in caprine pneumonia. In total 244 pneumonic goat lung samples collected over a 10-year period (2003)(2004)(2005)(2006)(2007)(2008)(2009)(2010)(2011)(2012) were screened for the isolation and identification of associated mollicutes. The identification was done on the basis of biochemical tests, growth inhibition test, PCR, and sequencing. The most frequent isolates were Acholeplasma (20/244, i.e. 8.19%) and Mycoplasma arginini (13/244, i.e. 5.3%), followed by M. mycoides subsp. capri (1/244) and M. agalactiae (1/244). Seven representative isolates of M. arginini were subjected to partial sequencing of the rpoB gene. On phylogenic analysis, M. arginini isolates were found to be highly identical, exhibiting >98% identity with that of the standard M. arginini (ATCC 23243) strain. The results encourage exploration of the role of M. arginini in goat pneumonia in much detail, due to its high frequency involvement.

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Multilocus sequence typing of pathogenic Mycoplasma mycoides subsp. capri reveals the predominance of a novel clonal complex among isolates from goats in India

et al. 2020

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Genetic diversity of Mycoplasma arginini isolates based on multilocus sequence typing

Cited by 15 publications

References 20 publications

Antimicrobial susceptibility and multilocus sequence typing of Mycoplasma capricolum subsp. capricolum

Antimicrobial susceptibility and multilocus sequence typing of Mycoplasma capricolum subsp. capricolum

Mycoplasma arginini: high frequency involvement in goat pneumonia

Multilocus sequence typing of pathogenic Mycoplasma mycoides subsp. capri reveals the predominance of a novel clonal complex among isolates from goats in India

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