Genetic diversity of Mycobacterium tuberculosis isolates at the Military Medical Academy in Ankara, Turkey

Kısa, Özgül; Albay, Ali; Baylan, Orhan; Tozkoparan, Ergün; Açıkel, Cengiz Han; Doğancı, Levent

doi:10.1016/j.resmic.2007.01.004

Cited by 7 publications

(13 citation statements)

References 26 publications

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“…In this study, spoligotyping of the 95 drug-resistant M. tuberculosis clinical isolates resulted in a very high clustering rate (79%). This clustering rate was similar to the results of the previous studies used spoligotyping (77%, 76%, 85%) in Turkey [8], [13], [14]. These rates are closer to the rates reported in Harare, Zimbabwe (84.1%) and in Finland (90%), but much higher than the rates of clustered drug-resistance cases observed in studies performed in Sweden (23.6%) and India (43.5%) [9], [17], [18], [29].…”

Section: Discussionsupporting

confidence: 91%

“…Two of these studies showed that LAM7-TUR lineage corresponded to the most frequent spoligotype pattern SIT41 (21% and 22.5%, respectively) in the respective study populations [8], [16]; which allowed the description of a new phylogeographically-specific clone of M. tuberculosis , designated LAM7-TUR [16]. In another study from Ankara performed on 114 isolates, the frequency of this spoligotype was 7.9% and it was the 2 nd most prevalent spoligotype pattern [14]. A retrospective analysis of a paper on 374 isolates from Turkey published in 2005, i.e., a year before the designation LAM7-TUR was officially published, showed that as high as 14.4% of M. tuberculosis isolates belonged to LAM7-TUR which stood as the 2 nd most common spoligotype [13].…”

Section: Discussionmentioning

confidence: 97%

“…Major spoligotyping-based lineages of M. tuberculosis clinical isolates from different geographical areas of Turkey were assessed in previous studies [8], [14], [16]. Two of these studies showed that LAM7-TUR lineage corresponded to the most frequent spoligotype pattern SIT41 (21% and 22.5%, respectively) in the respective study populations [8], [16]; which allowed the description of a new phylogeographically-specific clone of M. tuberculosis , designated LAM7-TUR [16].…”

Section: Discussionmentioning

confidence: 99%

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Distribution of Spoligotyping Defined Genotypic Lineages among Drug-Resistant Mycobacterium tuberculosis Complex Clinical Isolates in Ankara, Turkey

Kısa

Tarhan²,

Günal

et al. 2012

PLoS ONE

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BackgroundInvestigation of genetic heterogeneity and spoligotype-defined lineages of drug-resistant Mycobacterium tuberculosis clinical isolates collected during a three-year period in two university hospitals and National Tuberculosis Reference and Research Laboratory in Ankara, Turkey.Methods and FindingsA total of 95 drug-resistant M. tuberculosis isolates collected from three different centers were included in this study. Susceptibility testing of the isolates to four major antituberculous drugs was performed using proportion method on Löwenstein–Jensen medium and BACTEC 460-TB system. All clinical isolates were typed by using spoligotyping and IS6110-restriction fragment length polymorphism (RFLP) methods. Seventy-three of the 95 (76.8%) drug resistant M. tuberculosis isolates were isoniazid-resistant, 45 (47.4%) were rifampicin-resistant, 32 (33.7%) were streptomycin-resistant and 31 (32.6%) were ethambutol-resistant. The proportion of multidrug-resistant isolates (MDR) was 42.1%. By using spoligotyping, 35 distinct patterns were observed; 75 clinical isolates were grouped in 15 clusters (clustering rate of 79%) and 20 isolates displayed unique patterns. Five of these 20 unique patterns corresponded to orphan patterns in the SITVIT2 database, while 4 shared types containing 8 isolates were newly created. The most prevalent M. tuberculosis lineages were: Haarlem (23/95, 24.2%), ill-defined T superfamily (22/95, 23.2%), the Turkey family (19/95, 20%; previously designated as LAM7-TUR), Beijing (6/95, 6.3%), and Latin-America & Mediterranean (LAM, 5/95 or 5.3%), followed by Manu (3/95, 3.2%) and S (1/95, 1%) lineages. Four of the six Beijing family isolates (66.7%) were MDR. A combination of IS6110-RFLP and spoligotyping reduced the clustering rate from 79% to 11.5% among the drug resistant isolates.ConclusionsThe results obtained showed that ill-defined T, Haarlem, the Turkey family (previously designated as LAM7-TUR family with high phylogeographical specifity for Turkey), Beijing and LAM were predominant lineages observed in almost 80% of the drug-Resistant M. tuberculosis complex clinical isolates in Ankara, Turkey.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

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Distribution of Spoligotyping Defined Genotypic Lineages among Drug-Resistant Mycobacterium tuberculosis Complex Clinical Isolates in Ankara, Turkey

Kısa

Tarhan²,

Günal

et al. 2012

PLoS ONE

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“…The results of spoligotyping performed by Durmaz et al (8) for drug-resistant strains obtained from different areas of our country showed that SIT41, with a rate of 23%, is the most frequent genotype. In the spoligotyping studies carried by Kisa et al (15) in Ankara (Capital city, Central Anatolia) and by Aktaş et al (13) in Zonguldak (Northern Turkey) SIT53 was reported to be the most frequent genotype (32% and 22%, respectively), followed by SIT41. However, in both studies, besides strains resistant to drugs, sensitive strains were also included.…”

Section: Discussionmentioning

confidence: 94%

“…In spoligotyping studies carried out in our country, the clustering rate ranges between 67-85% (6,8,(13)(14)(15)(16). This rate is unrealistically high.…”

Section: Discussionmentioning

confidence: 99%

Molecular Diversity of Drug Resistant Mycobacterium Tuberculosis Strains in Western Turkey

Sürücüoğlu¹,

Günal²,

Özkütük³

et al. 2012

Balkan Med J

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Objective: The aim of this study was to investigate the molecular diversity and clonal relationship of drug resistant Mycobacterium tuberculosis strains isolated in Western Turkey. Materials and Methods:A total of 87 strains isolated between 2006 and 2009, eight of which were rifampicin monoresistant and 79 were multidrug resistant, were analyzed with IS6110 RFLP and spoligotyping methods. Results:The results of spoligotyping showed that 7% of the strains were orphans, and 8% were undefined for family in the SpolDB4 database. Major families of the strains were LAM (38%), T (35%), Haarlem (7%), Beijing (2%), S (2%) and U (1%) families. The clustering rate by spoligotyping was calculated as 75%. The most predominant SIT cluster was SIT41 (29%). According to the results of IS6110 RFLP, 71 different patterns of IS6110 were observed. Low copy number was found in 26% of the strains. When the results of two methods were combined, the final clustering rate was calculated as 26%. Conclusions:The genotypical distribution of drug resistant tuberculosis isolates in our region indicates genetic diversity and the clustering rate was found low in our region. However, more comprehensive and long-term molecular epidemiological studies are needed to control the drug resistant strains.

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Spoligotyping and drug resistance patterns of Mycobacterium tuberculosis isolates from five provinces of Iran

et al. 2013

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Tuberculosis (TB) persists as a public health problem in Iran. Characterization of Mycobacterium tuberculosis isolates circulating in this area will contribute to understand and control the spread of the strains. The aims of this study were to understand the genetic diversity and drug susceptibility of M. tuberculosis isolates circulating in Iran and to analyze the relationship between genotype and drug resistance. A total of 291 M. tuberculosis isolates collected from TB patients were genotyped by spoligotyping. Drug susceptibility testing was performed using proportion method. Spoligotyping resulted in 75 distinct patterns. 86.2% of isolates were grouped in 35 clusters while the remaining isolates were unique. Ural was found to be the most predominant lineage (34.3%) followed by Central Asian strain (CAS) (24%), T (18.2%), Manu2 (7.5%) and Latin American-Mediterranean (LAM) (6.1%). The five largest clusters were Ural/Spoligotype International Type (SIT)127 (15.8%), CAS1/SIT26 (9.2%), T1/SIT53 (6.1%), T1/SIT284 (5.4%), and CAS1/SIT25 (4.4%). About 5% of isolates had multidrug resistance (MDR) and 10% had other resistance. MDR was significantly associated with Beijing strains, but not with Ural family. This study highlights dominance of Ural, CAS, and T families in Iran. Biogeographic specificity of CAS and T families to border provinces of Iran including Sistan-Baluchestan and Kermanshah, respectively, suggested that this family strains might be transmitted from these regions to other provinces of the country.

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Genetic diversity of Mycobacterium tuberculosis isolates at the Military Medical Academy in Ankara, Turkey

Cited by 7 publications

References 26 publications

Distribution of Spoligotyping Defined Genotypic Lineages among Drug-Resistant Mycobacterium tuberculosis Complex Clinical Isolates in Ankara, Turkey

Distribution of Spoligotyping Defined Genotypic Lineages among Drug-Resistant Mycobacterium tuberculosis Complex Clinical Isolates in Ankara, Turkey

Molecular Diversity of Drug Resistant Mycobacterium Tuberculosis Strains in Western Turkey

Spoligotyping and drug resistance patterns of Mycobacterium tuberculosis isolates from five provinces of Iran

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