Molecular Diversity of Drug Resistant Mycobacterium Tuberculosis Strains in Western Turkey

Abstract

Objective: The aim of this study was to investigate the molecular diversity and clonal relationship of drug resistant Mycobacterium tuberculosis strains isolated in Western Turkey. Materials and Methods:A total of 87 strains isolated between 2006 and 2009, eight of which were rifampicin monoresistant and 79 were multidrug resistant, were analyzed with IS6110 RFLP and spoligotyping methods. Results:The results of spoligotyping showed that 7% of the strains were orphans, and 8% were undefined for family in the SpolDB4 database. Major families of the strains were LAM (38%), T (35%), Haarlem (7%), Beijing (2%), S (2%) and U (1%) families. The clustering rate by spoligotyping was calculated as 75%. The most predominant SIT cluster was SIT41 (29%). According to the results of IS6110 RFLP, 71 different patterns of IS6110 were observed. Low copy number was found in 26% of the strains. When the results of two methods were combined, the final clustering rate was calculated as 26%. Conclusions:The genotypical distribution of drug resistant tuberculosis isolates in our region indicates genetic diversity and the clustering rate was found low in our region. However, more comprehensive and long-term molecular epidemiological studies are needed to control the drug resistant strains.

Genotype distribution of Mycobacterium tuberculosis in theAegean Region and associated demographic factors

“…In three different studies conducted on drug-resistant and multidrug-resistant TB isolates in Turkey, the most common type was ST41 with rates of 26%, 22.5%, and 16.8%, and ST53 was the second most common with rates of 14%, 19.5%, and 15.8% (15,16,18). In our study, the rate of MDR was 3%, six of the 12 multidrug-resistant…”

“…Although different studies on multidrug-resistant TB isolates in Turkey have found variations in the rate of the Beijing strains between 1.3% and 50% due to differences in their patient populations, all the studies have found very high MDR ratios, varying from 41.3% to 66.7% among the Beijing strains (15)(16)(17). In a study on 4069 M. tuberculosis strains isolated in İstanbul in 2002-2005, the prevalence of the Beijing genotype was 1.1% (46/4069), the MDR rate was 12.5% (510/4069), the Beijing genotype's prevalence among multidrug-resistant TB isolates was 3.7% (19/510), MDR was found in 41.3% (19/49) of the Beijing strains, and 10 out of the 19 Beijing strains were isolated from patients who were citizens of former Soviet Union countries (17).…”

Potential bacterial pathogens associated in wound infections at University Health Centre of a Southwest in Nigeria

“…ln this study, Staphylococcus aureus was the most predominant bacteria this is contrary to the finding of 23 but in consonance with reports of similar studies conducted from different parts of Nigeria 24,25,26, and other country , Turkey. 27 .…”

Genotyping of Mycobacterium tuberculosis Complex Isolates from Patients with Tuberculosis by MIRU-VNTR and Spoligotyping Methods

“…Yine ülkemizde yapılan çalışmalara baktığımızda SIT41 kümesi %23.9-7.9 (23)(24)(25)(26)(27)(28) arasında değişen oranlarda saptanmıştır. SIT41 kümesinin üç çalışmada en yaygın, iki çalışmada ise ikinci sıklıkla tespit edilen küme olduğu vurgulanmıştır.…”

“…Yaptığımız çalışmada ise SIT41 kümesi %11.6 oranında tespit edilmiş ve bu oran ile üçüncü en sık küme olarak bulunmuştur. Yapılan bu çalışmalarda Haarlem ailesi üçüncü ve dördüncü en sık tespit edilen aile olarak bildirilirken (23)(24)(25)(26)(27)(28) , bizim çalışmamızda ise bu aile ikinci en sık tespit edilen aile olmuştur (Tablo 2). Köksalan ve ark.…”