Genetic diversity of mango accessions (Mangifera indica) using new microsatellite markers and morphological descriptors

Abstract

Genetic diversity estimates based on morphological and molecular data can provide different information on the relationship between cultivars of a species. This study aimed to develop new microsatellite markers as additional tools in genetic studies on mangoes (Mangifera indica L.), and to analyze the genetic variability of 20 mango cultivars based on morphological descriptors and microsatellite markers. We aimed to better understand the cultivars enhanced breeding histories and to support crossbreeding planning. Positive clones were selected from a DNA library enriched for microsatellite regions for sequencing and primer design. Four plants of each of the 20 accessions were used for observations, based on 48 morphological descriptors. Twenty accessions were analyzed using 27 microsatellite markers, of which 16 were developed during this study. The clusters, based on the morphological descriptors by Ward-MLM strategy and the microsatellite markers, suggested that Brazilian mango cultivars have extensive genetic diversity and are related to cultivars with different provenances, demonstrating their different enhanced breeding histories.

Situación actual y perspectivas del cultivo de mango (Mangifera indica l.) en el Sur de Manabi, Ecuador

“…Está claro que la determinación basada exclusivamente en caracteres morfológicos no siempre es exacta debido a la influencia de los factores ambientales y al número limitado de caracteres distinguibles. De esta manera, la identificación molecular de las variedades de mango se llevaron a cabo con diferentes técnicas moleculares como el uso de isoenzimas (Degani et al, 1990, Aron et al, 1997, repeticiones en tándem del número variable (VNTR) (Adato et al, 1995, Krishna y Singh, 2007, Begum et al, 2012Sennhenn et al, 2014;Wang et al, 2016), polimorfismos de longitud en fragmentos amplificados (AFLPs) (Eiadthong et al, 2000;Kashkush et al, 2001;Yamanaka et al, 2006;Santos et al, 2008;Gálvez-López et al, 2009), ADN polimórficos amplificados al azar (RAPDs) (Souza et al, 2011;Samal et al, 2012;Hossain et al, 2016;Pruthvish y Chikkaswamy, 2016;Galal et al, 2017) y microsatélites (SSRs) (Surapaneni et al, 2013, Ravishankar et al, 2015, Azmat et al, 2016, Bajpai et al, 2016, dos Santos Alves et al, 2016, Nazish et al, 2017, Guerra et al, 2018. Los resultados revelan diferencias entre las accesiones de mango sin importar el sistema de marcador utilizado, su origen geográfico o su situación genética (cultivares, razas autóctonas o variedades).…”

In vitro conservation of mango (Mangifera indica L.) Ubá and Carlota cvs. through culturing immature embryos

“…For the purpose of supporting genetic improvement programs and effi cient germplasm conservation, various studies have been carried out to investigate the genetic diversity of mango, including the development of a genetic map (Samal et al 2012, Alves et al 2016, Kuhn et al 2017. Due to the importance of the crop and the large number of improvement programs, germplasm banks under field conditions are maintained by various institutions, in countries such as India, Brazil and Australia (Souza et al 2011, Dillon e al.…”