In vitro conservation of mango (Mangifera indica L.) Ubá and Carlota cvs. through culturing immature embryos

Souza, Fernanda Vidigal Duarte; RebouÇas, Celma C; Souza, Everton Hilo de; Peixoto, Clovis Pereira

doi:10.1590/0001-3765202020190400

Cited by 5 publications

(2 citation statements)

References 19 publications

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“…Studies for in vitro conservation pursuit protocols which may establish the reduction of plant metabolism, promoting lower growth rates, sub-cultivation in higher intervals of time, lower values of senescent leaves and higher number of green leaves (Mancilla-Álvarez et al 2019;Souza et al 2020). Therefore, the association between those variables is fundamental, as a way to identify minimum growth, preserving the viability of multiplication, recovery and rescue of plants (Lacerda et al 2020).…”

Section: Discussionmentioning

confidence: 99%

Effect of different temperatures for in vitro conservation of cassava (Manihot esculenta Crantz) germplasm

Santos¹,

Freitas²,

Souza³

et al. 2022

CLIUM

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The objective of the present work was to evaluate in vitro growth of cassava accessions under different temperatures to improve protocols for germplasm conservation. Acclimatized chambers were tested at 18 °C, 20 °C and 22 °C, and again at 22 °C in conservation room (control) in five accessions: Mandioca João Grande (BGM1325), Fécula Branca (BGM1632), BRS Kiriris (BGM1728), S/N (121) (BGM2004) and BRS Prata (BGM2019). After 90 days of distance, the following were evaluated: shoot height (cm), number of sprouts and microcuttings, number of green and senescent leaves, fresh and dry weight of shoots and roots (g). The experiment was performed under a completely randomized design in factorial scheme 5(accessions) x 4(temperatures), with 20 replicates. All data were submitted to the F test ANOVA and evaluated by the Tukey and Scott-Knott tests at 5% probability. The control temperature promoted lower mean values for plant shoot height, with emphasis for accessions BGM1325, BGM1632 and BGM2019. In a general manner accessions BGM1325, BGM1632 and BGM2019 showed lower SH, with the first and BGM2004 outstanding with the best results for lower NSL and higher NGL.

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Section: Discussionmentioning

confidence: 99%

Effect of different temperatures for in vitro conservation of cassava (Manihot esculenta Crantz) germplasm

Santos¹,

Freitas²,

Souza³

et al. 2022

CLIUM

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“…Furthermore, it is necessary to highlight the interaction between the cultural system and the media composition used. More recently, Souza et al [455] established an efficient protocol for in vitro rescue of immature embryos to preserve mango germplasm. The immature fruits were harvested at 13-20 DAP.…”

Section: Analysis Of Embryogenesis With Embryo Rescue Technical Holdermentioning

confidence: 99%

Embryo Rescue in Plant Breeding

Rogo,

Fambrini,

Pugliesi

2023

Plants

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Embryo rescue (ER) techniques are among the oldest and most successful in vitro tissue culture protocols used with plant species. ER refers to a series of methods that promote the development of an immature or lethal embryo into a viable plant. Intraspecific, interspecific, or intergeneric crosses allow the introgression of important alleles of agricultural interest from wild species, such as resistance or tolerance to abiotic and biotic stresses or morphological traits in crops. However, pre-zygotic and post-zygotic reproductive barriers often present challenges in achieving successful hybridization. Pre-zygotic barriers manifest as incompatibility reactions that hinder pollen germination, pollen tube growth, or penetration into the ovule occurring in various tissues, such as the stigma, style, or ovary. To overcome these barriers, several strategies are employed, including cut-style or graft-on-style techniques, the utilization of mixed pollen from distinct species, placenta pollination, and in vitro ovule pollination. On the other hand, post-zygotic barriers act at different tissues and stages ranging from early embryo development to the subsequent growth and reproduction of the offspring. Many crosses among different genera result in embryo abortion due to the failure of endosperm development. In such cases, ER techniques are needed to rescue these hybrids. ER holds great promise for not only facilitating successful crosses but also for obtaining haploids, doubled haploids, and manipulating the ploidy levels for chromosome engineering by monosomic and disomic addition as well substitution lines. Furthermore, ER can be used to shorten the reproductive cycle and for the propagation of rare plants. Additionally, it has been repeatedly used to study the stages of embryonic development, especially in embryo-lethal mutants. The most widely used ER procedure is the culture of immature embryos taken and placed directly on culture media. In certain cases, the in vitro culture of ovule, ovaries or placentas enables the successful development of young embryos from the zygote stage to maturity.

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Mango

Vincent,

Anushma

2023

Fruit and Nut Crops

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In vitro conservation of mango (Mangifera indica L.) Ubá and Carlota cvs. through culturing immature embryos

Cited by 5 publications

References 19 publications

Effect of different temperatures for in vitro conservation of cassava (Manihot esculenta Crantz) germplasm

Effect of different temperatures for in vitro conservation of cassava (Manihot esculenta Crantz) germplasm

Embryo Rescue in Plant Breeding

Mango

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