Genetic Diversity of Korean Cylindrocarpon destructans Based on Virulence Aassay and RAPD Analysis

Abstract

: Ginseng root rot caused by Cylindrocarpon destructans is one of the most destructive diseases of ginseng (Panax ginseng). We analyzed the features of the species through pathogenicity test and genetic diversity analysis of C. destructans in Korea, for its application as basic data to attempt for effective control. C. destructans isolated from rotted ginseng roots exhibited a variety of colonial colors on media. It was assumed that there may exist genetic diversity in the population by the diversity of pathogenicity among isolates observed when artificially inoculated into ginseng roots. Pathogenicity tests using ex vivo wound inoculation with agar mixture inoculation on ginseng roots were performed similar results as were observed appear to be useful for rapid pathogen inspection. According to RAPD analysis results, Korean C. destructans isolates formed a single genetic group which can be distinguished readily from closely related other fungi. C. destructans group was divided into two small groups. Therefore, we were able to confirm pathogenicity and genetic difference between the isolates in each of the groups of the pathogen.

Genetic Diversity and Population Structure of Cylindrocarpon-like Fungi Infecting Ginseng Roots in Northeast China

“…[ 11 ]. Genetic diversity of C. destructans from Asian ginseng in Korean has been associated with hosts based on RAPD finger printing or sequence analysis of nuclear ribosomal gene internal transcribed spacer (ITS) and mitochondrial small subunit (mt SSU) rDNA; however, in the study, only less than 20 isolates were investigated [ 12 , 13 ]. Although there are no genetic markers employed specifically in genetic study of CLF, several phylogenetic analyses of CLF showed that histone H3 ( his3 ) gene had the highest resolution among several gene loci [ 1 , 11 ].…”

Genetic Diversity and Pathogenicity of Cylindrocarpon destructans Isolates Obtained from Korean Panax ginseng

“…Recently, several attempts have been made to establish a new type of classification system for the Nectria / Neonectria complex and to analyze the genetic diversity using various morphological, pathogenic, and genetic analytical approaches [ 9 , 17 , 18 , 19 ]. Although the genetic variation of Korean C. destructans population that causes root rot in P. ginseng has been evaluated by random amplified polymorphic DNA analysis [ 20 , 21 ], the pathogenic and taxonomical characteristics of P. ginseng in Korea have been rarely studied. As the genetically distinct populations may show difference in host range, aggressiveness, and susceptibility to disease control treatment, a better understanding of the genetic and pathogenic variations in C. destructans will be important for developing suitable root rot disease management strategies [ 16 ].…”