Genetic Diversity of Indigofera tinctoria L. in Java and Madura Islands as Natural Batik Dye based on Inter-simple Sequence Repeat Markers

Abstract

Abstract. Indigo leaves (Indigofera tinctoria L.) are utilized as a source of batik dye in Java and Madura islands, Indonesia. Batik makers from the islands collect indigo leaves from wild plants. Continuous collection of wild plants from their natural habitat may decrease their diversity. The present research was conducted to analyze the genetic diversity and population structure of I. tinctoria L. from Java and Madura. 55 indigo accessions were collected from 10 locations in Java and Madura. The indigo DNA was extracted and amplified using 15 ISSR primers. A total of 123 bands showed 50-90% polymorphic loci. PCA and UPGMA were used to analyze the data. All indigo accessions were clustered into 4 groups at a 60% similarity coefficient: a group consisting of Madura accessions, a group consisting of East Java accessions, a group consisting of Central Java accessions, and a group consisting of West Java accessions. Accessions collected from the same or adjacent populations tended to show similar ISSR characteristics. The genetic diversity of indigo in Java and Madura islands is relatively high (%P = 68.3), but the diversity residing within each population is lower (37%) than that between populations (63%).

Comparative analysis of Genetic Diversity among Pod Vegetables Genetic Resources Potential in Indonesia Revealed by ISSR Markers

“…Hal ini berarti susunan basa dengan pola (AC) 8 TG memiliki sekuens komplemen lebih sedikit dibandingkan dengan susunan basa yang berpola (AC) 8 T pada genom sayuran polong. Primer PKBT 3 dan PKBT 6 merupakan primer informatif karena sekuen primer memilki banyak kecocokan dengan urutan basa mikrosatelit yang terdapat pada sampel tanaman maupun genom tanaman lainnya, seperti anggrek (Romeida et al, 2012), manggis (Sulassih et al, 2013) dan tarum (Hariri et al, 2017).…”

“…Hasil penelitian menunjukkan persentase pita polimorfik pada populasi interspesifik memiliki persentase polimorfis lebih tinggi (Thul et al, 2012) bila dibandingkan dengan populasi intraspesifik (Rana et al, 2014). Menurut Hariri et al (2017) Hasil analisis hubungan genetik pada tingkat interspesifik lebih menunjukkan keragaman yang lebih tinggi dibandingkan pada tingkat intraspesifik. Pada tingkat kemiripan 44.40-70.00% menunjukkan bahwa antar spesies sayuran polong berada pada kelompok yang berbeda, sedangkan pada tingkat kemiripan 91.25-97.50% menunjukkan bahwa antar aksesi dalam spesies yang sama berada pada kelompok yang berbeda.…”

Characterization of Rambutan Cultivars (Nephelium lappaceum L.) Based on Leaf Morphological and Genetic CharacterCharacterization of Rambutan Cultivars (Nephelium lappaceum) Based on Leaf Morphological and Genetic

“…DNA from dried rambutan leaves were extracted according to Cetyl Trimethyl Ammonium Bromide (CTAB) protocol (Doyle 1991) with several modifications by Hariri (2017), Souza et al (2012) and Riupassa (2016). The modification included the addition of Sorbitol buffer before CTAB buffer extraction to minimize the high mucus content of the leaves, and also the addition of repeated DNA purification process to separate polysaccharide and other secondary metabolite residues from the DNA samples.…”

“…The modification included the addition of Sorbitol buffer before CTAB buffer extraction to minimize the high mucus content of the leaves, and also the addition of repeated DNA purification process to separate polysaccharide and other secondary metabolite residues from the DNA samples. Six out of 31 ISSR primers (Degani et al 2003, Hariri 2017, Riupassa 2016 were selected to produce the clearest and showed the highest polymorphic INTRODUCTION Rambutan (Nephelium lappaceum L.) is one of tropical fruit that has a high potential value. It belongs to Sapindaceae which originated from South East Asia primarily in Indonesia and Malaysia (Leenhouts, 1986).…”

Morphological and Physiological Responses of Indigofera tinctoria L. to Light Intensity

“…tinctoria is from the Fabaceae family, which is efficacious as a natural dye industry. The plant is used as a natural dye agent in the textile industry because it contains indigo pigments that produce indigo colors [3]. I. tinctoria natural dyes were the main product of India and West Africa before the discovery of synthetic indigo.…”