Genetic diversity of Indian native cattle breeds as analysed with 20 microsatellite loci

Abstract

The aim of the present study was to assess the genetic variation and establish the relationship amongst the three Indian zebu cattle breeds using 20 bovine-specific microsatellite markers. A total of 136 unrelated DNA samples from Sahiwal (SC), Hariana (HC) and Deoni (DC) breeds of cattle were genotyped to estimate within and between breed genetic diversity indices. The estimated mean allelic diversity was 5.2, 6.5 and 5.9 in SC, HC and DC, respectively, with a total of 167 alleles. The average observed and expected heterozygosity for the population varied from 0.42 (SC) to 0.59 (DC), and from 0.61 (SC) to 0.70 (DC), respectively. Low values of genetic variability estimates were observed in SC when compared with DC and HC, indicating some loss of variability because of its relatively small population size. From global F-statistics a significant deficit of heterozygotes of 24.2% (p < 0.05) was observed for each one of the analysed breeds whereas the total population had a 32.8% (p < 0.05) deficit of heterozygotes. The F ST estimates demonstrated that approximately 88.7% of the total genetic variation was because of the genetic differentiation within each breed. Pair-wise breed differentiation, Nei's standard and D A genetic distance estimates revealed relatively close genetic similarity between HC and DC in comparison with SC. In the UPGMA-based phylogenetic tree constructed from the genetic distances, HC and DC were grouped together in one cluster and SC in the other. The estimated time of divergence suggested a separation time of approximately 776 years between DC and HC, and a comparatively longer period (1296 years) between DC and SC.

Characterization of rare migratory cattle and evaluation of its phylogeny using short-tandem-repeat-based markers

“…Microsatellites are best suited to evaluate genetic diversity (Metta et al 2004;Li et al 2005;Yoon et al 2005;Sodhi et al 2005Sodhi et al , 2006Pandey, Rekha, Singh, et al 2006, Pandey, Rekha, Yatender, et al 2006Sharma et al 2013) and relationships among cattle populations from Africa, Europe and Asia (Moazami-Goudarzi et al 1997;Machugh et al 1998;Martín-Burriel et al 1999;Mommens et al 1999;Beja-Pereira et al 2003;Jordana et al 2003). Using a set of bovine-specific microsatellite markers, Mukesh et al (2004) studied the genetic diversity (both within and between) and relationship among the three important Indian cattle breeds, namely Sahiwal, Hariana and Deoni. Moreover, microsatellite markers had also been used as suitable tool for linkage mapping, identification of quantitative trait loci, parentage determination (Bruford & Wayne 1993).…”

Relaciones entre los bovinos criollos panameños y algunas razas criollas de Latinoamérica

“…La correlación negativa entre los valores F ST y Nm permiten establecer de manera más precisa las relaciones entre poblaciones, particularmente en aquellas estrechamente relacionadas en términos evolutivos y donde la deriva genética ha sido un factor importante (Mukesh et al, 2004 (0,14).…”

Genetic Diversity of 76 STR Loci in the Dahe Pig

“…微卫星 DNA(microsatellite DNA)，也称为短串 联重复序列(short tandem repeats, STR)、 简单序列重 复 (simple sequence repeat, SSR) ， 因具有分布广泛、 多态性丰富、共显性遗传、选择中性、易于检测、 重复性好、可提供高分辨率的遗传信息等特点，在 度量品种群体遗传结构、遗传多样性等研究方面广 为应用 (Zhang & Zhang, 2001;Li et al, 2004;Mukesh et al, 2004;Kim et al, 2005) …”