Genetic diversity of Ralstonia solanacearum species complex strains obtained from Guangxi, China and their pathogenicity on plants in the Cucurbitaceae family and other botanical families

Abstract

Bacterial wilt, caused by the Ralstonia solanacearum species complex (RSSC), is one of the most destructive bacterial plant diseases. It is distributed worldwide but is more intense in tropical and subtropical regions (Mansfield et al., 2012). The RSSC infects the roots of plants through the soil and water and multiplies through the xylem, where it clogs the vascular tissues and ultimately collapses the host (Mansfield et al., 2012). This group of bacterial pathogens has an extensive host range and can infect over 450 plant species representing 54 botanical families (Hayward, 1991;Wicker et al., 2007). Members of the RSSC are highly diverse pathogens with multiple taxonomic

Whole genome sequencing suggests that “non-pathogenicity on banana (NPB)” is the ancestral state of the Ralstonia solanacearum IIB-4 lineage

“…Interestingly, UW70 was highly virulent on melons, but UA-1591 is reported to be avirulent on cucumber 8 [12]. Cucurbit pathogenicity has also been documented in phylotype I strains isolated in Taiwan, Thailand, China, and Japan [27][28][29][30].…”

“…In addition to host range variation within sequevar IIB-4, banana-virulent strains in IIA-24 and IIA-41 clades vary in their pathogenicity on tomato [25]. A study of phylotype I Ralstonia from Guanxi, China showed that closely related strains shared virulence on tomato, eggplant, tobacco, and potato but varied in their pathogenicity on pepper, mulberry, ginger, and cucurbits [27]. Host range patterns in the Ralstonia solanacearum species complex are complicated because they are generalists with limits to their host range.…”

“…Interestingly, UW70 was highly virulent on melons, but UA-1591 is reported to be avirulent on cucumber (Ramírez et al 2020). Cucurbit virulence has also been documented in phylotype I strains isolated in Taiwan, Thailand, China, and Japan (He et al 2021;Yahiaoui et al 2017;Horita et al 2014;Lin et al 2014).…”

“…In addition to host range variation within sequevar IIB-4, banana-virulent strains in IIA-24 and IIA-41 clades vary in their virulence on tomato (Albuquerque et al 2014). A study of phylotype I Ralstonia from Guanxi, China showed that closely related strains shared virulence on tomato, eggplant, tobacco, and potato but varied in their virulence on pepper, mulberry, ginger, and cucurbits (He et al 2021).…”

Weighted Gene Co-Expression Analysis Network-Based Analysis on the Candidate Pathways and Hub Genes in Eggplant Bacterial Wilt-Resistance: A Plant Research Study

“…R. solanacearum (EP01) [48] was provided by the plant pathology laboratory of Guangxi University. Its specificity and phylotypes were identified by phylotype-specific multiplex PCR using a set of phylotype-specific primers and species-specific primers (Additional file S1: Table S10) and 2 × A8 FastHiFi PCR Mastermix amplification (Aidlab, Beijing, China).…”