Genetic Diversity of Pyrenophora teres Isolates as Detected by AFLP Analysis

Abstract

Amplified fragment length polymorphism (AFLP) analysis has been used to analyse mainly 83 Czech isolates of Pyrenophora teres, P. graminea, P. tritici-repentis and Helminthosporium sativum. Each species had distinct AFLP profiles. Using 19 primer combinations 948 polymorphic bands were detected. All main clusters in dendrogram correspond to the studied species. Even the two forms of P. teres -P. teres f. teres (PTT) and P. teres f. maculata (PTM) -formed different clusters. Genetic diversity, with regard to the locality and the year of the sample's collection, was analysed separately within the AFLP-based dendrogram cluster of PTT and PTM. Unweighted pairgroup method (UPGMA) analysis of the 37 isolates of PTT and 30 isolates of PTM, using 469 polymorphic bands, showed that the variability seemed to have been influenced more by the year of sampling than by the geographic origin of the isolate. The presence of intermediate haplotypes with a relatively high number of shared markers between the two groups indicated that hybridization between the forms of P. teres could happen, but it is probably often overlapped by selection pressure or genetic drift.www.blackwell-synergy.com

Pyrenophora tritici-repentis, Causal Agent of Tan Spot: A Review of Intraspecific Genetic Diversity

“…However, at present exists different studies where the aim consists in the characterization of genetic variability of Pyrenophora tritici-repentis population in different localities, areas and countries. (Ali et al, 2010;Andrie et al, 2007;Friesen et al, 2005;Leisová et al, 2008;Lepoint et al, 2010;Litcher et al, 2002;Mehta et al, 2004;Moreno et al, 2009; Pujol Vieira dos Santos et al 2002; Singh & Hughes, 2006).…”

“…Several studies included tools for detection variability www.intechopen.com using of morphocultural, pathogenic, bioquemical and molecular variability. Several studies to detect variability include different tools, the most of them used pathogenic and genetic characterization (PCR techniques) (Ali et al, 2010;Andrie et al, 2007;Friesen et al, 2005;Leisová et al, 2008;Lepoint et al, 2010;Litcher et al, 2002;Mehta et al, 2004;Moreno et al, 2009;Pujol Vieira dos Santos et al 2002;Singh & Hughes 2006). A lesser extent of research focuses on characterization based on morphological and biochemical aspects (Diaz de Ackermann 1987; Gilchrist et al, 1984;Hosford, 1971;Hunger & Brown 1987, Moreno et al, 2008aPujol Vieira dos Santos et al, 2002).…”

“…It is a robust and reliable technique, based on the high reproducibility, and on the homology between bands of the same weight. In the case of causal agent of tan spot, only two researches worked with this technique (Bentata et al, 2011;Friesen et al, 2005;Leisová et al, 2008). Friesen et al, (2005), presented a study of genetic diversity of global collection of P. tritici-repentis.…”

“…AFLP, have been used in different works for characterize the population of different Pyrenophora species (Friesen et al, 2005;Leisová et al, 2005Leisová et al, , 2008Serenius et al, 2007). This procedure is based on the selective amplification of genomic restriction fragments.…”

“…Within them Amplified Fragment Length Polymorphism (AFLP) (Friesen et al, 2005;Leisová et al, 2008), Enterobacterial Repetitive Intergenic Consensus (ERIC) and Repetitive Extragenic Palindromic (REP)-PCR technique (Mehta et al, 2004), Internal Transcribed Spacer Regions (ITS) (Lepoint et al, 2010), Mating Type locus (MAT) (Lepoint et al, 2010), Inter Simple Sequence Repeat (ISSR) (Moreno et al, 2009) and Random Amplified Polymorphisms DNA (RAPD) (Pujol Vieira dos Santos et al, 2002;Singh & Hughes, 2006), Restriction Fragment Length Polymorphism (RFLP), Toxin production genes (Tox A, Tox B and tox b genes) (Ali et al, 2010;Andrie et al, 2007;Lepoint et al, 2010).…”