Genetic diversity of<i>Babesia</i>in<i>Ixodes persulcatus</i>and small mammals from North Ural and West Siberia, Russia

Рар, В. А.; Epikhina, Tamara; Ливанова, Н. Н.; Panov, Victor V.

doi:10.1017/s0031182010001162

Cited by 47 publications

(36 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, unlike I. persulcatus, in which PCR detected the B. microti U.S. lineage, all sympatric I. ovatus ticks tested were negative. This specific I. persulcatus-U.S. lineage relationship was observed in previous studies in Japan (33) and Russia (31). In the Novosibirsk region in Russia, where the B. microti U.S. lineage is distributed in voles, mice, and shrews, I. persulcatus ticks are found to carry U.S. lineages.…”

Section: Discussionsupporting

confidence: 84%

“…In the Eurasian region where I. scapularis is absent, PCR surveillance and DNA sequence analyses of field-collected ticks have revealed that Ixodes persulcatus (31)(32)(33) and Ixodes ricinus (1,34,35) in Asia and Europe, respectively, carry the B. microti U.S. lineage. Although experimental transmission of B. microti U.S. lineage to hamsters or gerbils has been shown (33,36), there is still a lack of biological evidence demonstrating the live pathogen in field-derived specimens.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Ixodes persulcatus Ticks as Vectors for the Babesia microti U.S. Lineage in Japan

Zamoto-Niikura

Morikawa

Hanaki

et al. 2016

Appl Environ Microbiol

View full text Add to dashboard Cite

The U.S. lineage, one of the major clades in the Babesia microti group, is known as a causal agent of human babesiosis mostly in the northeastern and upper midwestern United States. This lineage, however, also is distributed throughout the temperate zone of Eurasia with several reported human cases, although convincing evidence of the identity of the specific vector(s) in this area is lacking. Here, the goal was to demonstrate the presence of infectious parasites directly in salivary glands of Ixodes persulcatus, from which U.S. lineage genetic sequences have been detected in Asia, and to molecularly characterize the isolates. Five PCRpositive specimens were individually inoculated into hamsters, resulting in infections in four; consequently, four strains were newly established. Molecular characterization, including 18S rRNA, ␤-tubulin, and CCT7 gene sequences, as well as Western blot analysis and indirect fluorescent antibody assay, revealed that all four strains were identical to each other and to the U.S. lineage strains isolated from rodents captured in Japan. The 18S rRNA gene sequence from the isolates was identical to those from I. persulcatus in Russia and China, but the genetic and antigenic profiles of the Japanese parasites differ from those in the United States and Europe. Together with previous epidemiological and transmission studies, we conclude that I. persulcatus is likely the principal vector for the B. microti U.S. lineage in Japan and presumably in northeastern Eurasia. IMPORTANCEThe major cause of human babesiosis, the tick-borne blood parasite Babesia microti, U.S. lineage, is widely distributed in the temperate Northern Hemisphere. However, the specific tick vector(s) remains unidentified in Eurasia, where there are people with antibodies to the B. microti U.S. lineage and cases of human babesiosis. In this study, the first isolation of B. microti U.S. lineage from Ixodes persulcatus ticks, a principal vector for many tick-borne diseases, is described in Japan. Limited antigenic cross-reaction was found between the Japan and United States isolates. Thus, current serological tests based on U.S. isolates may underestimate B. microti occurrence outside the United States. This study and previous studies indicate that I. persulcatus is part of the B. microti U.S. lineage life cycle in Japan and, presumably, northeastern Eurasia. This report will be important for public health, especially since infection may occur through transfusion, and also to researchers in the field of parasitology.

show abstract

Section: Discussionsupporting

confidence: 84%

mentioning

confidence: 99%

Ixodes persulcatus Ticks as Vectors for the Babesia microti U.S. Lineage in Japan

Zamoto-Niikura

Morikawa

Hanaki

et al. 2016

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…(rodents), and Sorex spp. (shrew) in Eurasia, including Japan (2,4,9,21,37,38). The two regional taxa of the Hobetsu and Munich, biologically inseparable from U.S. parasites but distinctive phylogenetically by the ␤-tubulin and CCT7 gene analyses, are common in Japan and Europe, respectively.…”

mentioning

confidence: 99%

Detection of Two Zoonotic Babesia microti Lineages, the Hobetsu and U.S. Lineages, in Two Sympatric Tick Species, Ixodes ovatus and Ixodes persulcatus, Respectively, in Japan

Zamoto-Niikura

Tsuji

Wang

et al. 2012

Appl Environ Microbiol

View full text Add to dashboard Cite

ABSTRACTThe speciesBabesia microti, commonly found in rodents, demonstrates a high degree of genetic diversity. Three lineages, U.S., Kobe, and Hobetsu, are known to have zoonotic potential, but their tick vector(s) in Japan remains to be elucidated. We conducted a field investigation at Nemuro on Hokkaido Island and at Sumoto on Awaji Island, where up to two of the three lineages occur with similar frequencies in reservoirs. By flagging vegetation at these spots and surrounding areas, 4,010 ticks, comprising six species, were collected. A nested PCR that detects the 18S rRNA gene ofBabesiaspecies revealed thatIxodes ovatusandI. persulcatusalone were positive. Lineage-specific PCR for rRNA-positive samples demonstrated thatI. ovatusandI. persulcatuscarried, respectively, the Hobetsu and U.S. parasites. No Kobe-specific DNA was detected. InfectedI. ovatusticks were found at multiple sites, including Nemuro and Sumoto, with minimum infection rates (MIR) of ∼12.3%. However, allI. persulcatusticks collected within the same regions, a total of 535, were negative for the Hobetsu lineage, indicating thatI. ovatus, but notI. persulcatus, was the vector for the lineage. At Nemuro, U.S. lineage was detected in 2 of 139 adultI. persulcatusticks (MIR, 1.4%), for the first time, while 48 ofI. ovatusticks were negative for that lineage. Laboratory experiments confirmed the transmission of Hobetsu and U.S. parasites to hamsters viaI. ovatusandI. persulcatus, respectively. Differences in vector capacity shown by MIRs at Nemuro, where the two species were equally likely to acquire either lineage of parasite, may explain the difference in distribution of Hobetsu throughout Japan and U.S. taxa in Nemuro. These findings are of importance in the assessment of the regional risk for babesiosis in humans.

show abstract

“…Babesia DNA was detected by nested PCR based on the 18S ribosomal RNA (rRNA) gene as previously described (Rar et al 2011). Primary reactions were carried out using the forward primer BS1 (5′-gacggtagggtattggcct-3′) and reverse primer BS2 (5′-attcaccggatcactcgatc-3′).…”

Section: Dna Extraction Pcr and Sequencingmentioning

confidence: 99%

“…B. microti 'US' type was detected in I. persulcatus ticks, small rodents, and shrews from I. persulcatus distributive area in Siberia and the Far East (Zamoto et al 2004;Rar et al 2010Rar et al , 2011Rar et al , 2014a. In contrast, small mammals from I. persulcatus/ Ixodes trianguliceps sympatric areas located in Ural and West Siberia were shown to be infected with B. microti 'Munich' type or with both B. microti 'US' type and 'Munich' type (Rar et al 2011(Rar et al , 2014bNefedova et al 2012).…”

Section: Introductionmentioning

confidence: 99%

High prevalence of Babesia microti ‘Munich’ type in small mammals from an Ixodes persulcatus/Ixodes trianguliceps sympatric area in the Omsk region, Russia

et al. 2016

View full text Add to dashboard Cite

Babesia microti is a genetically diverse group of protozoan parasites whose life cycle is associated with both small mammals and Ixodes spp. ticks. In this study, the prevalence of different B. microti genetic groups in ticks and small rodents in an area with Ixodes persulcatus and Ixodes trianguliceps occurring in sympatry was examined. A total of 541 small mammals were captured during eight sampling periods between 2013 and 2015 at a site in the Omsk region of Russia and tested for the presence of B. microti using nested PCR with subsequent sequencing of positive samples. B. microti DNA was found in 31.6 % of examined samples, and prevalence rates ranged from 5.3 to 61.6 % in different sampling periods. The sequenced B. microti samples belonged to two genetic groups: enzootic B. microti 'Munich' type and zoonotic B. microti 'US' type. B. microti 'Munich' type was more common across all sampling periods, with greater than 80 % prevalence in infected animals. Despite the high B. microti 'Munich'-type prevalence in voles, B. microti was not found in any of 394 adult I. persulcatus ticks collected by flagging or in the 84 I. persulcatus or 20 I. trianguliceps ticks taken from voles and molted under laboratory conditions. It was demonstrated that B. microti 'Munich'-type DNA can be detected in the blood of naturally infected voles for at least 20 weeks after capture. Thus, the high prevalence of B. microti 'Munich' type in small mammals may be explained by the prolonged persistence of B. microti in the blood of wild voles.

show abstract

Genetic diversity ofBabesiainIxodes persulcatusand small mammals from North Ural and West Siberia, Russia

Abstract: To our knowledge, this is the first detection of Babesia sensu stricto in I. persulcatus ticks and of B. microti in I. persulcatus in the Asian part of Russia.

Cited by 47 publications

References 32 publications

Ixodes persulcatus Ticks as Vectors for the Babesia microti U.S. Lineage in Japan

Ixodes persulcatus Ticks as Vectors for the Babesia microti U.S. Lineage in Japan

Detection of Two Zoonotic Babesia microti Lineages, the Hobetsu and U.S. Lineages, in Two Sympatric Tick Species, Ixodes ovatus and Ixodes persulcatus, Respectively, in Japan

High prevalence of Babesia microti ‘Munich’ type in small mammals from an Ixodes persulcatus/Ixodes trianguliceps sympatric area in the Omsk region, Russia

Contact Info

Product

Resources

About