Genetic diversity of human parvovirus B19: sequence analysis of the VP1/VP2 gene from multiple isolates

Abstract

To evaluate the genetic variability of human parvovirus B19, the complete coding region of the VP1/VP2 structural proteins of 29 B19 isolates obtained from 25 infected patients were sequenced and compared with each other and with two previously published B19 isolates. The VP1/VP2 gene was amplified by PCR using B19-specific oligonucleotide primers and the amplification products were sequenced directly. Overall, the average nucleotide and predicted amino acid identity among B19 isolates was high. Sequential virus isolates from the same cases and isolates obtained from two cases linked by transmission in the same household were essentially identical. Sequence variation was minimal among isolates obtained from a single community-wide B19 outbreak, ranging between 0 and 10 (0,4%) base substitutions, although there appeared to be more than one genetic lineage circulating in the outbreak. A comparison with 18 additional isolates from distinct epidemiological settings found greater variability. These isolates differed from each other by between 11 (0.5%) and 112 (4.8%) base substitutions. B19 isolates from Xi'an, China, were significantly different from other isolates at both the nucleoticle and amino acid levels, and were more closely related to a single isolate from Japan, obtained 10 years earlier, than to isolates from other countries. Isolates examined in this study included distinct genotypes from patients with similar clinical presentations and similar genotypes from patients with diverse clinical presentations. These data suggest that geographically defined genetic lineages of B19 may exist and that no particular B19 genotype was associated with a particular clinical outcome.

Genetic Diversity within Human Erythroviruses: Identification of Three Genotypes

“…Partial sequence data from different coding regions of the viral genome have confirmed this high degree of similarity with a larger number of isolates (12,15,23,25,48). For instance, sequence variation of the VP1/VP2 gene has been reported to be very low among B19 virus isolates obtained from a single community-wide outbreak (0 to 0.6% base substitutions) and only slightly greater among B19 virus isolates obtained from distinct epidemiological settings and geographical area, ranging between 0.5 and 4.8% for the most distant isolates (12). B19 virus genomes recovered from synovial tissue during persistent infection have also been reported to be very similar to those recovered from the same tissue during acute infection and to those recovered from blood or bone marrow (25).…”

Genetic variability of porcine parvovirus isolates revealed by analysis of partial sequences of the structural coding gene VP2

“…This result suggests that different genotypes may co-exist in a given region. In a similar work, Erdman et al (1996), while evaluating the genetic diversity of human parvovirus B19, suggest that more than one genetic lineage appears to circulate in an outbreak.…”

Endothelium-Dependent Flow-Mediated Vasodilation of Systemic Arteries Is Impaired in Patients With Myocardial Virus Persistence

“…Sequence analysis of PVB19 PCR fragments was performed by an automatic ABI Prism 310 Genetic Analyzer and BigDye Cycle Sequencing Kit according to the manufacturer instructions (Applied Biosystems). 27 …”