Genetic diversity of Histoplasma and Sporothrix complexes based on sequences of their ITS1-5.8S-ITS2 regions from the BOLD System

Estrada-Bárcenas, Daniel Alfonso; Vite-Garín, Tania; Navarro-Barranco, Hortensia; Torre-Arciniega, Raúl de la; Pérez-Mejía, Amelia; Rodríguez-Arellanes, Gabriela; Ramírez, José Antonio; Sahaza, Jorge H.; Taylor, Mária Lucía; Toriello, Conchita

doi:10.1016/j.riam.2013.10.003

Cited by 13 publications

(5 citation statements)

References 16 publications

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“…Internal transcribed spacer (ITS) is a universal barcode marker used for fungal identification [ 152 , 153 , 154 , 155 ]. DNA barcoding was first applied in 2003 by Hebert et al [ 156 ], and since then several studies have demonstrated that the polymorphisms in the regions flanking the 5.8S rDNA can be successfully used to distinguish fungal species [ 152 , 153 , 154 ].…”

Section: Molecular Diagnosismentioning

confidence: 99%

Trends in Molecular Diagnostics and Genotyping Tools Applied for Emerging Sporothrix Species

Carvalho

Monteiro

Hagen

et al. 2022

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Sporotrichosis is the most important subcutaneous mycosis that affects humans and animals worldwide. The mycosis is caused after a traumatic inoculation of fungal propagules into the host and may follow an animal or environmental transmission route. The main culprits of sporotrichosis are thermodimorphic Sporothrix species embedded in a clinical clade, including S. brasiliensis, S. schenckii, S. globosa, and S. luriei. Although sporotrichosis occurs worldwide, the etiological agents are not evenly distributed, as exemplified by ongoing outbreaks in Brazil and China, caused by S. brasiliensis and S. globosa, respectively. The gold standard for diagnosing sporotrichosis has been the isolation of the fungus in vitro. However, with the advance in molecular techniques, molecular assays have complemented and gradually replaced the classical mycological tests to quickly and accurately detect and/or differentiate molecular siblings in Sporothrix. Nearly all techniques available for molecular diagnosis of sporotrichosis involve PCR amplification, which is currently moving towards detecting Sporothrix DNA directly from clinical samples in multiplex qPCR assays. From an epidemiological perspective, genotyping is key to tracing back sources of Sporothrix infections, detecting diversity in outbreak areas, and thus uncovering finer-scale epidemiological patterns. Over the past decades, molecular epidemiological studies have provided essential information to policymakers regarding outbreak management. From high-to-low throughput genotyping methods, MLSA, AFLP, SSR, RAPD, PCR-RFLP, and WGS are available to assess the transmission dynamics and sporotrichosis expansion. This review discusses the trends in the molecular diagnosis of sporotrichosis, genotyping techniques applied in molecular epidemiological studies, and perspectives for the near future.

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Section: Molecular Diagnosismentioning

confidence: 99%

Trends in Molecular Diagnostics and Genotyping Tools Applied for Emerging Sporothrix Species

Carvalho

Monteiro

Hagen

et al. 2022

JoF

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“…In recent years (since 2014), several researchers have used molecular methods to identify Sporothrix species [53,[71][72][73]. The most informative loci used for species recognition are located in regions encoding proteins such as calmodulin [74,75], beta-tubulin [74,76,77], the Translation Elongation Factor [4,64,77], and the "fungal barcoding" regions (the ribosomal internal transcribed spacers) [78,79].…”

Section: Discussionmentioning

confidence: 99%

Sporotrichosis in Mexico

et al. 2020

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Sporotrichosis is an endemic mycosis caused by the species of the Sporothrix genus, and it is considered one of the most frequent subcutaneous mycoses in Mexico. This mycosis has become a relevant fungal infection in the last two decades. Today, much is known of its epidemiology and distribution, and its taxonomy has undergone revisions. New clinical species have been identified and classified through molecular tools, and they now include Sporothrix schenckii sensu stricto, Sporothrix brasiliensis, Sporothrix globosa, and Sporothrix luriei. In this article, we present a systematic review of sporotrichosis in Mexico that analyzes its epidemiology, geographic distribution, and diagnosis. The results show that the most common clinical presentation of sporotrichosis in Mexico is the lymphocutaneous form, with a higher incidence in the 0–15 age range, mainly in males, and for which trauma with plants is the most frequent source of infection. In Mexico, the laboratory diagnosis of sporotrichosis is mainly carried out using conventional methods, but in recent years, several researchers have used molecular methods to identify the Sporothrix species. The treatment of choice depends mainly on the clinical form of the disease, the host’s immunological status, and the species of Sporothrix involved. Despite the significance of this mycosis in Mexico, public information about sporotrichosis is scarce, and it is not considered reportable according to Mexico’s epidemiological national system, the “Sistema Nacional de Vigilancia Epidemiológica.” Due to the lack of data in Mexico regarding the epidemiology of this disease, we present a systematic review of sporotrichosis in Mexico, between 1914 and 2019, that analyzes its epidemiology, geographic distribution, and diagnosis.

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“…Thanks to the PCR techniques, sporotrichosis has been related with the theory of evolution, because types 3 and 4 (mtDNA) shows a worldwide distribution. Japanese researchers think that ancestral organisms derived in the course of continental separation and this divergence in South Africa occurred before than in Australia [ 32 ].…”

Section: Molecular Biology Diagnosismentioning

confidence: 99%