Sporotrichosis: From KOH to Molecular Biology

Arenas, Roberto; Sánchez-Cárdenas, Carlos Daniel; Ramírez-Hobak, Lourdes; Arriaga, Leon Felipe Ruíz; Memije, Ma Elisa Vega

doi:10.3390/jof4020062

Cited by 36 publications

(63 citation statements)

References 39 publications

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“… 10 The gold standard for diagnosis of Sporothrix was culture positive after incubation on Sabouraud dextrose agar for 5–8 days. 11 However, the infection lodge deep in host tissue, reside in cyst form, and the culture was frequently negative. Then tissue biopsy was usually required.…”

Section: Discussionmentioning

confidence: 99%

Conjunctival sporotrichosis from cat to human: Case report

Reinprayoon

Jermjutitham

Tirakunwichcha

et al. 2020

American Journal of Ophthalmology Case Reports

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Purpose To report a case of conjunctival plaque infected from Sporothrix schenckii , which was possible transmitted from a cat. Observations A case of Thai woman presented with chronic painless ulcerated conjunctiva with white plaque. The lesion was not healed for one month after excision. She became concerned of getting infection from her cat because it developed chronic and multiple skin lesions on its legs. Identification of Sporothrix schenckii by PCR method was confirmed in the tissues from her conjunctiva and cat's skin. Conclusion and Importance The diagnosis of Sporothrix infection should be suspected in atypical conjunctival lesion in patient who had closed contact with infected cat. This brought awareness to doctors and cat owners, even though it was uncommon.

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Section: Discussionmentioning

confidence: 99%

Conjunctival sporotrichosis from cat to human: Case report

Reinprayoon

Jermjutitham

Tirakunwichcha

et al. 2020

American Journal of Ophthalmology Case Reports

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“…The laboratory diagnosis of the disease in felines begins with the cytology by imprint of the lesions, followed by isolation of the agent in culture. The latter is considered the gold standard [9]. Other methods, such as those based on investigation of serum antigens and antibodies, have been studied since the 1970s [10,11], although none involved a laboratory routine or proved to be clinically validated.…”

Section: Introductionmentioning

confidence: 99%

Promising application of the SsCBF ELISA test to monitor the therapeutic response of feline sporotrichosis caused by Sporothrix brasiliensis from Brazilian epidemics

et al. 2020

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Sporotrichosis zoonotic transmission by cats has obtained hyperendemic magnitude in Rio de Janeiro, Brazil. Atypical cases, relapses, and reinfections as well as reduced diagnostic sensitivity of conventional methods have been reported. Previously, the anti-SsCBF enzyme-linked immunosorbent assay (ELISA) test was shown to be useful as a diagnostic tool for human sporotrichosis. Effective diagnosis and treatment are critical to interrupt the chain of transmission of this major pathogen in Brazilian Public Health. To evaluate its applicability for feline sporotrichosis diagnosis and/or therapeutic follow-up, 15 domestic cats from Rio de Janeiro were clinically and laboratory monitored by cytopathology, culture, Sporothrix genotyping, and anti-SsCBF IgG levels. Subsequently, animals were divided into satisfactory and non-satisfactory therapeutic responders. Averages of antibody serum levels obtained for diagnosis (first consultation) compared with the levels found after follow-up (last consultation) were significantly different in both groups (p = 0.0002 and p = 0.038, respectively). We conclude that the SsCBF ELISA test can predict feline sporotrichosis therapeutic responses even for animals with distinct clinical evolutions.

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“…There are cases in which this disease is not suspected and culture tests are therefore not performed at the time of skin biopsy. In addition, microscopic examination after applying potassium hydroxide to the purulent exudate from the lesion or histopathological studies is not informative enough to establish a diagnosis of sporotrichosis, as the classical cigar‐shaped fungal cells or asteroid bodies characteristic of sporotrichosis are detected in only a small number of positive samples …”

Section: Introductionmentioning

confidence: 99%

“…In addition, microscopic examination after applying potassium hydroxide to the purulent exudate from the lesion or histopathological studies is not informative enough to establish a diagnosis of sporotrichosis, as the classical cigar-shaped fungal cells or asteroid bodies characteristic of sporotrichosis are detected in only a small number of positive samples. 5 In 2003, Hu et al 6 reported PCR diagnosis of Sporothrix infection from skin tissue. According to their method, a skin tissue sample taken at biopsy was frozen, then DNA was extracted from the cryopreserved tissue, and nested PCR was performed using primers specific for Sporothrix.…”

Section: Introductionmentioning

confidence: 99%

Diagnostic value of a nested polymerase chain reaction for diagnosing cutaneous sporotrichosis from paraffin‐embedded skin tissue

Hayashi

Kaminaga

Baba

et al. 2019

Mycoses

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Summary Background The gold standard for diagnosis of cutaneous sporotrichosis involves the isolation of the fungus, Sporothrix, by a culture test. Generally, the sampling for the culture test is performed at the same time as skin biopsy under local anaesthesia. However, the culture test may occasionally return a false negative result. Objective The aim of our study was to investigate the diagnostic value of a molecular method for diagnosing cutaneous sporotrichosis from formalin‐fixed and paraffin‐embedded (FFPE) tissues. Methods Over a 30‐year period, we collected 52 cases of cutaneous sporotrichosis from biopsied specimens that had been positively diagnosed by a culture test. A nested PCR specific for Sporothrix detection was applied using FFPE tissue as template. The results were compared with control samples from 79 patients diagnosed with other cutaneous diseases according to histopathological, clinical findings and a cutler test. Results Of the 52 patients who were tested positive on the culture test, all cutaneous diseases were detected by PCR. Of the 59 patients in the control group, 58 tested negative by PCR. Under our conditions, the calculated sensitivity of this method was 100%, the specificity was 98.7% and the kappa coefficient was 0.984 (95% CI: 0.953‐1.000). Conclusions The specific PCR assay used appears to be a useful tool for the prompt and accurate diagnosis of sporotrichosis. Using this method, it would be possible to diagnose cutaneous sporotrichosis for patients who were suspected of cutaneous sporotrichosis but tested negative on culturing, and for pathologically suspected cutaneous sporotrichosis patients for whom the culture test was not undertaken.

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Sporotrichosis: From KOH to Molecular Biology

Cited by 36 publications

References 39 publications

Conjunctival sporotrichosis from cat to human: Case report

Conjunctival sporotrichosis from cat to human: Case report

Promising application of the SsCBF ELISA test to monitor the therapeutic response of feline sporotrichosis caused by Sporothrix brasiliensis from Brazilian epidemics

Diagnostic value of a nested polymerase chain reaction for diagnosing cutaneous sporotrichosis from paraffin‐embedded skin tissue

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