Genetic diversity of Gram-negative, proteolytic, psychrotrophic bacteria isolated from refrigerated raw milk

Martins, Maurílio Lopes; Pinto, Carina Aparecida; Rocha, Rodrigo Barros; Araújo, Elza Fernandes de; Vanetti, Maria Cristina Dantas

doi:10.1016/j.ijfoodmicro.2006.06.020

Cited by 68 publications

(50 citation statements)

References 20 publications

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“…Milk is a complex substrate that can support the growth of a wide variety of both Grampositive and Gram-negative bacteria, as well as that of yeast and moulds (Lafarge et al 2004;Martins et al 2006). Milk pasteurization is a common practice for safeguarding consumers from food-borne pathogenic bacteria (Ranieri et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Diversity and biofilm-forming capability of bacteria recovered from stainless steel pipes of a milk-processing dairy plant

Cherif-Antar

Moussa–Boudjemâa

Didouh

et al. 2015

Dairy Sci. & Technol.

104

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Bacteria may adhere to and develop biofilm structures onto dairy surfaces trying to protect themselves from adverse conditions such as pasteurization and CIP processes. Thus, biofilms are considered common sources of food contamination with undesirable bacteria. The purpose of this study was to evaluate the diversity of the microbiota attached to stainless steel surfaces in pre-and post-pasteurization pipe lines of a milk-processing plant. Seventy Gram-positive isolates were identified as Enterococcus faecalis (33), Bacillus cereus (26), Staphylococcus hominis (8), Staphylococcus saprophyticus (2), and Staphylococcus epidermidis-Staphylococcus aureus (1) species. Fifty-five Gram-negative isolates were identified to the species Escherichia coli (18), Klebsiella pneumoniae (13), Acinetobacter calcoaceticus (6), Serratia marcescens (6), Enterobacter spp. (5), Pseudomonas aeruginosa (4), Escherichia vulneris (2), and Proteus mirabilis (1). Fifty-five different strains were detected by the RAPD technique. These were subjected to an in vitro assay to evaluate their biofilm-forming capability. E. faecalis (7), A. calcoaceticus (4), K. pneumoniae (3), S. hominis (3), and P. aeruginosa (2) were the species in which more biofilm producer strains were encountered. The adhered microbiota was also assessed by the PCR-DGGE culture-independent technique. This analysis revealed a greater bacterial diversity than that revealed by culturing methods. In this way, in addition to the bacteria detected by culturing, DNA bands belonging to the genera Chrysobacterium and Streptomyces were also identified. This study emphasizes that knowledge of attached Dairy Sci. & Technol. (2016) 96:27-38 DOI 10.1007

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Section: Introductionmentioning

confidence: 99%

Diversity and biofilm-forming capability of bacteria recovered from stainless steel pipes of a milk-processing dairy plant

Cherif-Antar

Moussa–Boudjemâa

Didouh

et al. 2015

Dairy Sci. & Technol.

104

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“…Pseudomonas, Achromobacter, Aeromonas, Serratia, Alcaligenes, Chromobacterium, Flavobacterium and Enterobacter etc. (Ryser, 1999;Martins et al, 2006;Torkar and Teger, 2008).…”

Section: Discussionmentioning

confidence: 99%

Phosphatase Activity of Microbial Populations in Different Milk Samples in Relation to Protein and Carbohydrate Content

et al. 2014

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Cattle milk is a rich source of protein, carbohydrate, vitamins, minerals and all other major and micronutrients. At a moderate pH, milk is an excellent media for the growth of microbes and thus, intake of raw milk is precarious. In this study, attempt was made for a qualitative study of eight raw milk samples of different varieties of cow and goat milk, collected from Jorhat district of Assam, India, on the basis of nutritional value and microbial population. The highest microbial population was found in the milk collected from cross hybrid variety of cow, whereas microbial contamination was the least in Jersey cow milk. Samples of C1 (Jersey cow) variety showed presence of the highest amount of protein and carbohydrate content as compared to the others. Almost all the milk samples showed positive acid and alkaline phosphatase activity. Maximum acid phosphatase activity was observed in cross hybrid cow milk, whereas local cow milk exhibited the highest alkaline phosphatase activity. Phosphatase activity did not show any co-relationship with microbial population of the milk samples. Similarly, the protein and carbohydrate content of the samples did not have any significant impact on both acid and alkaline phosphatase activity.

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“…Seventeen primers were tested for efficacy in the RAPD method, and the primers listed in Table 2 were selected because they gave reproducible results. The RAPD was performed as modified according to Martins et al (2006). The amplification reactions were accomplished in a final volume of 25 ml containing 50mM Tris-HCl (pH 8.3), 3mM MgCl 2 , 0.4mM deoxyribonucleotide triphosphates (dNTP), 0.4mM of each primer, 1 U Taq DNA polymerase (Promega, Madison, USA), and 50 ng/ml of DNA.…”

Section: Methodsmentioning

confidence: 99%

Characterising the genetic diversity of Pseudomonas syringae pv. syringae isolated from rice and wheat in Iran

Dariush¹,

Ebadi²,

Khoshkdaman³

et al. 2012

Plant Prot. Sci.

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Dariush S., Ebadi A.A., Khoshkdaman M., Rabiei B., Elahinia A. (2012): Characterising the genetic diversity of Pseudomonas syringae pv. syringae isolated from rice and wheat in Iran. Plant Protect. Sci., 48: 162-169.Sheath rot of rice and leaf blight of wheat caused by Pseudomonas syringae pv. syringae are the important bacterial pathogens of rice and wheat in Iran. The randomly amplified polymorphic DNA (RAPD) method was used to investigate the genetic diversity of 60 strains of P. s. pv. syringae obtained from rice and wheat in different growth stages. Cluster analysis by UPGMA method showed that strains were grouped into two clusters. The AMOVA analysis indicated that about 18% of the total genetic variation existed between two populations of rice and wheat, which showed the lack of host specialization in P. s. pv. syringae strains among rice and wheat. We confirmed that high genetic heterogeneity existed in the P. s. pv. syringae strains which are detectable by RAPD analysis, and that molecular and statistical analysis of RAPD fragments can be used both to distinguish between strains and to determine relatedness between them.

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Genetic diversity of Gram-negative, proteolytic, psychrotrophic bacteria isolated from refrigerated raw milk

Cited by 68 publications

References 20 publications

Diversity and biofilm-forming capability of bacteria recovered from stainless steel pipes of a milk-processing dairy plant

Diversity and biofilm-forming capability of bacteria recovered from stainless steel pipes of a milk-processing dairy plant

Phosphatase Activity of Microbial Populations in Different Milk Samples in Relation to Protein and Carbohydrate Content

Characterising the genetic diversity of Pseudomonas syringae pv. syringae isolated from rice and wheat in Iran

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