Characterising the genetic diversity of Pseudomonas syringae pv. syringae isolated from rice and wheat in Iran

Dariush, Somayeh; Ebadi, Ali; Khoshkdaman, Maryam; Rabiei, Babak; Elahinia, Ali

doi:10.17221/64/2011-pps

Cited by 4 publications

(4 citation statements)

References 29 publications

(26 reference statements)

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“…The genetic heterogeneity of P. syringae pv. syringae strains was examined by many authors (Vicente, Roberts, 2007;Abbasi et al, 2011;Dariush et al, 2012). Various molecular techniques have been used to characterize P. syringae pv.…”

Section: Molecular Characterization Of Pseudomonas Syringae Pvs Frommentioning

confidence: 99%

Molecular characterization of Pseudomonas syringae pvs. from different host plants by repetitive sequence-based PCR and multiplex-PCR

Iličić

Balaž

Stojšin

et al. 2016

Zemdirbyste-Agriculture

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Pseudomonas syringae pvs., isolated from different cultivars of sweet cherry grown in several locations in Serbia, were characterized and compared with strains collected earlier from sour and sweet cherry and oil pumpkin growing in this region, as well as with reference strains P. syringae pv. morsprunorum race 1 CFBP2119, P. s. pv. lachrymans 765R and P. s. pv. syringae H-1. By employing LOPAT and GATTa tests, isolates were identified as P. syringae pv. syringae and P. s. pv. morsprunorum race 1. Simultaneous detection of syringomycin synthesis (syrB) and syringomycin secretion (syrD) genes in multiplex-polymerase chain reaction (m-PCR) was used for P. syringae pv. syringae confirmation. All isolates detected as P. syringae pv. morsprunorum race 1 after biochemical characterization were positive for cfl gene amplification. Using a repetitive sequence-based PCR (rep-PCR) both syringae and morsprunorum race 1 pathovars were clustered separately, with 42% similarity. No significant differences between isolates in each pathovar were noted, although they were collected from different sweet cherry cultivars and varying localities. The most similar to the P. syringae pv. syringae isolates was strain T6 with 19% similarity, followed by strain Tk21 from oil pumpkin -25%. The isolates of both pathovars were detected on the same location (Selenca, Serbia) and the same cultivar ('Merchant'), albeit in two different years.

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Section: Molecular Characterization Of Pseudomonas Syringae Pvs Frommentioning

confidence: 99%

Molecular characterization of Pseudomonas syringae pvs. from different host plants by repetitive sequence-based PCR and multiplex-PCR

Iličić

Balaž

Stojšin

et al. 2016

Zemdirbyste-Agriculture

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“…The genome size of Pseudomonas syringae is 6 093 698 bp with 59.2% GC content . It causes halo blight disease, forming small water‐soaked spots on the lower and upper leaf blades, leading to dryness of leaves. Xanthomonas oryzae species has a genome size of 5 238 555 bp with 63.6% GC content.…”

Section: Introductionmentioning

confidence: 99%

“…14 It causes halo blight disease, forming small water-soaked spots on the lower and upper leaf blades, leading to dryness of leaves. 15 Xanthomonas oryzae species has a genome size of 5 238 555 bp with 63.6% GC content. 16,17 This causes blight and leaf streak with yellowing and drying of rice plant leaves.…”

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confidence: 99%

Comparative genome‐wide analysis of codon usage of different bacterial species infecting Oryza sativa

Vasanthi

Dass

2018

J of Cellular Biochemistry

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Oryza sativa is vastly affected by microbial pathogen, causing blight-related diseases, which in turn deplete the growth and productivity of rice. In this study, we analyzed four bacterial rice pathogen genomes and reported on their codon usage that might have greater implication in mutation-related research. Differential codon usage indices, such as codon adaptation index (CAI), codon bias index (CBI), effective number of codons (ENc), relative synonymous codon usage (RSCU), correspondence analysis (COA), and parity plots, were applied on coding sequences of Pseudomonas fuscovaginae, Pseudomonas syringae, Xanthomonas oryzae, and Pseudomonas avenae speices. The RSCU results proposed a high-frequency usage of CUG and CGC that codes for leucine and arginine in all of the species. The CBI and CAI values between the genomes range from 0.17 to 0.3 and from 0.26 to 0.35, respectively, indicating a direct proportionality between these indexes. The mean ENc value of P. avenae coding sequence showed high codon bias compared with other genomes. The axis I variation from COA analysis shows a mean value of 42.28% codon variations in these bacterial species. Correlation studies between axis I and ENc-GC3, along with CAI and CBI, suggested the presence of nucleotide bias and mutational pressure as major forces for codon bias within these species. Hence, certain genes with high CAI-CBI have been correlated for better gene expression. Our study highlights the importance of nucleotide biasness, mutation pressure, and natural selection in shaping protein-coding genes in these four rice-affecting bacteria. This would further help in investigating the evolution of pathogenic gene families, which may direct research toward synthetic genes that could be suppressed or overrepresented based on their codon usage pattern toward pathogenicity.

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“…syringae van Hall 1902 (Pss) is considered to be one of the most important pathovars of this species with a wide host range infecting more than 180 plant species belonging to various genera (Bultreys and Kaluzna 2010). Strains of Pss cause important diseases such as bacterial canker of stone fruits, citrus blast, leaf blight of wheat and barley, sheath rot of rice, red streak of sugarcane and brown spot of bean (Kaluzna et al 2010;Dariush et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Journal of Plant Protection Research

Khezri

Mohammadi

2018

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Pseudomonas syringae pv. syringae (Pss) constitutes a diverse group of bacterial strains that cause canker of stone fruits, blight of cereals and red streak of sugarcane. The purpose of this study was to determine how diverse Iranian strains of Pss are when they come from different hosts. We compared a total of 32 Pss strains isolated from stone fruits, barley, wheat and sugarcane from different geographical regions of Iran based on their phenotypic and molecular properties. Strains showed some variation regarding carbon and nitrogen utilization. Pss strains were similar in their protein banding patterns. Additional bands were found in sugarcane strains. Most strains showed one indigenous plasmid DNA and a few had two and some none. The genes of syrB and syrD encoding syringomycin synthesis and secretion, respectively, were amplified using specific primers in polymerase chain reaction. Syringomycin, producing strains amplified two DNA fragments of 752 and 446 bp representing syrB and syrD genes, respectively. Primer specificity was shown for Pss using various genera. Based on the results of this study, it is suggested that Pss strains from different hosts and geographical regions show diversity in phenotypic and molecular characters. It is thought that phenotypic variation is due to adaptation to specific hosts and niches for survival and pathogenicity.

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Characterising the genetic diversity of Pseudomonas syringae pv. syringae isolated from rice and wheat in Iran

Cited by 4 publications

References 29 publications

Molecular characterization of Pseudomonas syringae pvs. from different host plants by repetitive sequence-based PCR and multiplex-PCR

Molecular characterization of Pseudomonas syringae pvs. from different host plants by repetitive sequence-based PCR and multiplex-PCR

Comparative genome‐wide analysis of codon usage of different bacterial species infecting Oryza sativa

Journal of Plant Protection Research

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