Genetic Diversity of G3 Rotavirus Strains Circulating in Argentina during 1998–2012 Assessed by Full Genome Analyses

Degiuseppe, Juan Ignacio; Parra, Gabriel I.; Stupka, Juan Andrés

doi:10.1371/journal.pone.0110341

Cited by 18 publications

(14 citation statements)

References 52 publications

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“…The RV vaccine have been shown to improve the health and well-being of children in countries where it has been introduced [ 47 , 48 , 49 , 50 ], leading to rapid and substantial drops in hospitalization and deaths due to RV infections [ 51 , 52 , 53 ]. Although the immunity induced by the vaccine protects against severe RV disease, re-infections by different genotypes of RV can occur throughout life as these viruses have a high genetic diversity [ 54 , 55 , 56 ]. This is a concern as several studies have reported the emergence of new RV strains that are not covered by the vaccines.…”

Section: Introductionmentioning

confidence: 99%

Global Occurrence and Emission of Rotaviruses to Surface Waters

et al. 2015

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Group A rotaviruses (RV) are the major cause of acute gastroenteritis in infants and young children globally. Waterborne transmission of RV and the presence of RV in water sources are of major public health importance. In this paper, we present the Global Waterborne Pathogen model for RV (GloWPa-Rota model) to estimate the global distribution of RV emissions to surface water. To our knowledge, this is the first model to do so. We review the literature to estimate three RV specific variables for the model: incidence, excretion rate and removal during wastewater treatment. We estimate total global RV emissions to be 2 × 1018 viral particles/grid/year, of which 87% is produced by the urban population. Hotspot regions with high RV emissions are urban areas in densely populated parts of the world, such as Bangladesh and Nigeria, while low emissions are found in rural areas in North Russia and the Australian desert. Even for industrialized regions with high population density and without tertiary treatment, such as the UK, substantial emissions are estimated. Modeling exercises like the one presented in this paper provide unique opportunities to further study these emissions to surface water, their sources and scenarios for improved management.

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Section: Introductionmentioning

confidence: 99%

Global Occurrence and Emission of Rotaviruses to Surface Waters

et al. 2015

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“…Residues of our G3P[9] differed most from the vaccine, particularly on the 7-1b antigenic epitope. Interestingly, the K238N substitution, which is also present in various G3 strains from Argentina, Belgium, Pakistan, and Lebanon [ 16 , 17 , 61 – 63 ], can potentially introduce an N-linked glycosylation in the 7-1b epitope and reduce antibody neutralization [ 64 ].…”

Section: Discussionmentioning

confidence: 99%

“…The broad host range for G3 including feline, canine, equine, porcine, lapine, bovine, rats, rhesus, and bats has resulted in the occasional detection of animal-like RVA infection in patients with diarrhea [ 14 , 19 – 24 ]. Although G3 is often found in combination with P[8], P[4], and P[6], pairings with P[9] and P[10] are less frequently reported [ 17 , 21 , 25 – 29 ]. Previously, we conducted surveillances of RVA infection in Thailand from 2015 to 2019 and found that G3 strains were most predominantly detected in adults and children with viral diarrhea [ 30 – 32 ].…”

Section: Introductionmentioning

confidence: 99%

Diverse human and bat-like rotavirus G3 strains circulating in suburban Bangkok

2022

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Although rotavirus vaccines are available in many parts of the world and are effective in reducing the overall incidence of rotavirus infection, it remains a major cause of diarrhea in less-developed countries. Among various rotavirus group A (RVA) strains, the increasingly common genotype G3 (defined by the VP7 gene) has been identified in both humans and animals. Our previous epidemiological surveillance in Bangkok found several unusual non-vaccine-like G3 strains in patients with diarrhea. In this study, we sequenced and characterized the genomes of seven of these G3 strains, which formed combinations with genotypes P[4], P[6], P[9], and P[10] (defined by the VP4 gene). Interestingly, we identified a bat-like RVA strain with the genome constellation G3-P[10]-I3-R3-C3-M3-A9-N3-T3-E3-H6, which has not been previously reported in the literature. The amino acid residues deduced from the nucleotide sequences of our G3 strains differed at the antigenic epitopes to those of the VP7 capsid protein of the G3 strain in RotaTeq vaccine. Although it is not unusual for the segmented genomes of RVA to reassort and give rise to emerging novel strains, the atypical G3 strains identified in this study suggest possible animal-to-human RVA zoonotic spillover even in urban areas.

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“…Phylogenetic tree was constructed using neighbor joining method (with 1000 bootstrap replicates) and distances were calculated with Kimura 2-parameter method using MEGA 5 [ 38 ]. Lineage designation for phylogenetic trees of G3 and P[8] were based on criteria reported earlier [ 19 , 39 ]. Structural analysis of VP7 (PBD 3FMG) and VP8* (PDB 1KQR) were performed using the UCSF Chimera-Molecular Modeling System [ 40 ].…”

Section: Methodsmentioning

confidence: 99%

High prevalence of G3 rotavirus in hospitalized children in Rawalpindi, Pakistan during 2014

et al. 2018

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Rotavirus A species (RVA) is the leading cause of severe diarrhea among children in both developed and developing countries. Among different RVA G types, humans are most commonly infected with G1, G2, G3, G4 and G9. During 2003–2004, G3 rotavirus termed as “new variant G3” emerged in Japan that later disseminated to multiple countries across the world. Although G3 rotaviruses are now commonly detected globally, they have been rarely reported from Pakistan. We investigated the genetic diversity of G3 strains responsible RVA gastroenteritis in children hospitalized in Rawalpindi, Pakistan during 2014. G3P[8] (18.3%; n = 24) was detected as the most common genotype causing majority of infections in children less than 06 months. Phylogenetic analysis of Pakistani G3 strains showed high amino acid similarity to “new variant G3” and G3 strains reported from China, Russia, USA, Japan, Belgium and Hungary during 2007–2012. Pakistani G3 strains belonged to lineage 3 within sub-lineage 3d, containing an extra N-linked glycosylation site compared to the G3 strain of RotaTeqTM. To our knowledge, this is the first report on the molecular epidemiology of G3 rotavirus strains from Pakistan and calls for immediate response measures to introduce RV vaccine in the routine immunization program of the country on priority.

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Genetic Diversity of G3 Rotavirus Strains Circulating in Argentina during 1998–2012 Assessed by Full Genome Analyses

Cited by 18 publications

References 52 publications

Global Occurrence and Emission of Rotaviruses to Surface Waters

Global Occurrence and Emission of Rotaviruses to Surface Waters

Diverse human and bat-like rotavirus G3 strains circulating in suburban Bangkok

High prevalence of G3 rotavirus in hospitalized children in Rawalpindi, Pakistan during 2014

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