Genetic diversity of four protected indigenous chicken breeds in China using microsatellite markers

Abstract

The genetic diversity of four protected indigenous chicken breeds was evaluated with 25 microsatellite markers. Polymorphism information content (PIC), heterozygosity with the estimator of genetic differentiation F ST and Nei's genetic distance were evaluated. The results showed that these four protected local chicken populations showed high levels of diversity. The proportion of inter-population subdivision among the four protected local chicken populations was 16.0%. The average heterozygosity was 0.514, 0.581, 0.567 and 0.589 in Dongan, Xuefeng black-bone, Xianghuang and Taoyuan chickens, respectively, while the average PIC estimates were 0.455, 0.581, 0.557 and 0.576. A phylogenetic tree was constructed using genetic distance and the neighbour-joining method. Its topology reflects the general pattern of genetic differentiation among the four chicken breeds. The results also showed high genetic diversity and genetic variation among all the breeds. The information about the four local breeds estimated by microsatellite analysis may be useful as an initial guide for the effective conservation of chicken genetic diversity and developing conservation strategies.

Genetic diversity of Saudi native chicken breeds segregating for naked neck and frizzle genes using microsatellite markers

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Genotypic characterization of ten microsatellite loci in two Brazilian free range (Caipira) chicken lines

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Genetic and Productive Studies on Egyptian Local and Exotic Laying Hen Breeds

“…Genomic DNA was extracted from blood using AXYGEN kit (Axygen Scientific, inc. USA). Ten microsatellite markers (HUJ7, MCW222, ADL268, MCW154, LEI0234, MCW216, ADL278, MCW295, LEI094 and LEI166) were selected based on the chicken genome database (Pandey et al, 2005;Honkatukia et al, 2013;Wei et al, 2013;Goto et al, 2014). Four markers were in linkage with production traits; HUJ7 and MCW154 with egg production while LEI0166 and LEI0094 were associated with growth traits.…”